Molecular identification marker for fertile cytoplasm of clustery tabasco pepper and maintainer line breeding method

Abstract

The invention provides a molecular identification marker for fertile cytoplasm of clustery tabasco pepper and a maintainer line breeding method. The method disclosed by the invention comprises the following steps: cultivating etiolated seedlings of fine varieties of clustery tabasco pepper by utilizing seeds, extracting mitochondria DNA, and screening varieties with fertile cytoplasm genotype N according to a molecular marker Mt_InDel10 in the invention; finally, performing flowering period hybridization on 100% of male sterility line materials and listed varieties with the fertile cytoplasm genotype N, rapidly breeding the male sterility line and maintainer line with excellent apparent characters according to fertility segregation conditions of hybrid offspring, and providing parent stockfor the male sterility three-line conveyance crossbreeding breeding. According to the breeding method disclosed by the invention, molecular marker screening and the conventional crossbreeding are combined, the blindness of the conventional breeding is reduced, the breeding age limit is shortened, the directive selection effect is improved, the breeding process is accelerated, and the molecular identification marker has important application value for accelerating improvement and promotion of excellent local varieties of the clustery tabasco pepper.

Description

technical field [0001] The invention belongs to the technical field of breeding, and in particular relates to a molecular identification marker of fertile cytoplasm of tufted pepper and a method for selecting a maintainer line. Background technique [0002] my country has a wide range of pepper cultivation areas and is the second largest exporter of processed peppers in the world after India. Clustered Chaotian pepper is one of the important types of processed pepper, which is a variety of pepper. Clustered Chaotian pepper has been highly valued all over the world because of its wide adaptability, rich nutrient components, and extensive processing and utilization. It has a significant effect on improving people's living standards and increasing farmers' economic income. At present, there are many local varieties of clustered Chaotian peppers, such as 'Sanyingjiao', 'Bullet', 'Cherry Spicy' from Jianshui, Yunnan Province, and 'Jixin Spicy' from Yunnan and Guizhou. These var...

AI Technical Summary

Problems solved by technology

Ji Jiaojiao (2014) and so on through Chili ( Capsicum annuum L.) was subjected to mitochondrial DNA extraction, polyacrylamide gel electrophoresis and Tail-PCR technology to develop a specific marker SCAR 130 , this marker can identify the male sterile type cytoplasm of pepper; however, the application of this marker needs to wait until the flower bud stage, the cycle is long, and the operation is relatively complicated

However, there is no research report on the identification of the fertile cytoplasm of the tufted pepper, and there is no method for rapid selection of the maintainer line.

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