Molecular identification marker for fertile cytoplasm of clustery tabasco pepper and maintainer line breeding method

A technology of molecular identification and chaotian pepper, which is applied in the field of breeding, can solve the problems of long cycle, no method for rapid selection of maintainer lines, complicated operation, etc., and achieve the effect of short cycle, accelerated female parent breeding process, and simple operation

Active Publication Date: 2018-11-20
QINGDAO AGRI UNIV
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Problems solved by technology

Ji Jiaojiao (2014) and so on through Chili ( Capsicum annuum L.) was subjected to mitochondrial DNA extraction, polyacrylamide gel electrophoresis and Tail-PCR technology to develop a specific marker SCAR 130 , this marker can identify the male sterile type cytoplasm of pepper; however, the appl

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  • Molecular identification marker for fertile cytoplasm of clustery tabasco pepper and maintainer line breeding method
  • Molecular identification marker for fertile cytoplasm of clustery tabasco pepper and maintainer line breeding method
  • Molecular identification marker for fertile cytoplasm of clustery tabasco pepper and maintainer line breeding method

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Embodiment 1

[0039] 1. Preparation of local varieties of clustered Chaotian pepper with excellent traits:

[0040] Excellent local varieties of clustered Chaotian pepper can be selected by plant selection. The selection results in the field are more, the fruit is correct, the growth potential is strong, and there is no disease and pest. The standard single plant with the characteristics of this variety, after selection, insert the logo and use it as a seed plant. After the fruit is red and ripe, it is harvested, and the seeds are numbered, photographed and registered in a net bag, and the important economic characteristics are registered. After that, the seeds are cut open, and then dried in the sun. More than 500 full seeds are taken and stored for later use. Get wherein 150 seeds carry out the cultivation of next step etiolated seedlings.

[0041] The local varieties in the present invention refer to: the local varieties are under the local natural environment conditions, through long-te...

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Abstract

The invention provides a molecular identification marker for fertile cytoplasm of clustery tabasco pepper and a maintainer line breeding method. The method disclosed by the invention comprises the following steps: cultivating etiolated seedlings of fine varieties of clustery tabasco pepper by utilizing seeds, extracting mitochondria DNA, and screening varieties with fertile cytoplasm genotype N according to a molecular marker Mt_InDel10 in the invention; finally, performing flowering period hybridization on 100% of male sterility line materials and listed varieties with the fertile cytoplasm genotype N, rapidly breeding the male sterility line and maintainer line with excellent apparent characters according to fertility segregation conditions of hybrid offspring, and providing parent stockfor the male sterility three-line conveyance crossbreeding breeding. According to the breeding method disclosed by the invention, molecular marker screening and the conventional crossbreeding are combined, the blindness of the conventional breeding is reduced, the breeding age limit is shortened, the directive selection effect is improved, the breeding process is accelerated, and the molecular identification marker has important application value for accelerating improvement and promotion of excellent local varieties of the clustery tabasco pepper.

Description

technical field [0001] The invention belongs to the technical field of breeding, and in particular relates to a molecular identification marker of fertile cytoplasm of tufted pepper and a method for selecting a maintainer line. Background technique [0002] my country has a wide range of pepper cultivation areas and is the second largest exporter of processed peppers in the world after India. Clustered Chaotian pepper is one of the important types of processed pepper, which is a variety of pepper. Clustered Chaotian pepper has been highly valued all over the world because of its wide adaptability, rich nutrient components, and extensive processing and utilization. It has a significant effect on improving people's living standards and increasing farmers' economic income. At present, there are many local varieties of clustered Chaotian peppers, such as 'Sanyingjiao', 'Bullet', 'Cherry Spicy' from Jianshui, Yunnan Province, and 'Jixin Spicy' from Yunnan and Guizhou. These var...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11A01H1/02A01H1/04
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13
Inventor 林多姜童王辉
Owner QINGDAO AGRI UNIV
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