Breeding and application of cytoplasmic male sterility restoring line of brassica napus rapeseed and radish

A Brassica napus, male sterility technology, applied in the directions of application, botanical equipment and methods, plant genetic improvement, etc., can solve the problem of no similar application, no application report of double low radish cytoplasmic male sterility restorer, and no See application in production and other issues, to achieve the effect of broad application prospects and simple breeding procedures

Inactive Publication Date: 2012-10-03
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Li Xufeng from Sichuan Union University, etc. used chromosome engineering to introduce the Ogucms restorer gene in the radish variety Orchid chinensis into rapeseed. Fertility has been well restored. This is the first Ogucms restoration source found from the Asian radish genome, but it has not been us

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: The rapeseed S1628 whose preservation number is CCTCC NO:P201203 was sown on October 2, with a sowing density of 8000 plants / mu, and the flowering stage in April of the following year was selected for selfing of a single plant with excellent economic characteristics, and after 6 years of continuous selfing Through directional selection, an excellent and stable inbred line (S118) was bred; the inbred line S118 was crossed with the cytoplasmic male sterile line of Brassica napus radish, and all F1 were fertile, which showed that S118 has a cytoplasmic male sterile line of Brassica napus radish Restoring genes.

Embodiment 2

[0026] Example 2: Breeding of a Restorer Line of Cytoplasmic Male Sterility in Brassica napus Radish

[0027] (1) The inbred line S118 with radish cytoplasmic male sterility restoration gene was used as the female parent, and the excellent double-low winter rapeseed varieties approved in my country (Zhongshuang 2, Huashuang 3, Huyou 17, Zunyou 1 or Zheyou 758) is a cross for the reincarnated male parent;

[0028] (2) Plant the seeds harvested in step (1) in divisions, investigate the cold resistance in winter and spring, select a frost-resistant individual plant with excellent economic characteristics for selfing at the flowering stage, and harvest the seeds when the rapeseed matures;

[0029] (3) Perform quality analysis on the seeds harvested in step (2), and select individual seeds with an oil content greater than 63%, erucic acid less than 1%, and glucosinolates less than 30 μmol / g;

[0030] (4) Continue planting the selected seeds in step (3) by dividing plants, select a ...

Embodiment 3

[0034] Example 3: Production of Hybrids Using Rapeseed Radish Cytoplasmic Male Sterility Restorer Line

[0035] In this example, the radish cytoplasmic male sterile lines 2006-1A, 2006-2A, 2006-3A, 2006-4A, 2006-5A, 2006-6A, the maintainer lines Zhongshuang 2, C -9, Zhongshuang No. 6, Zhongshuang No. 7, Yuhuang No. 1, and Youyan No. 7 are all materials known in the art (Zhongshuang No. 2, Zhongshuang No. 6, and Zhongshuang No. C-9 was bred by the Oil Plants Research Institute of the Academy, C-9 was bred by the Guizhou Oil Plants Institute, Yuhuang 1 was a new Brassica-type yellow-seeded hybrid rapeseed variety prepared by chemical killing in the Rapeseed Research Laboratory of the Department of Agronomy of Southwest Agricultural University, Youyan 7 The number is the first batch of double-low hybrid rape bred in the "Eighth Five-Year Plan" of the Guizhou Oil Plant Science Research Institute).

[0036] (1) The cytoplasmic male sterile lines 2006-1A (the maintainer line is Zho...

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PUM

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Abstract

The invention relates to a breeding method and application of a cytoplasmic male sterility restoring line of brassica napus rapeseed and radish. The method comprises the following steps of: choosing rapeseed S1628 with the preservation number of CCTCC NO: P201203, performing continuous inbreeding directional selection for six years to breed an excellent, high-oil and stable inbred line; using the cytoplasmic male sterility restoring line of the brassica napus rapeseed and radish as a female parent and using the bred excellent and stable inbred line as a male parent to select out strains comprising radish cytoplasmic male sterility restoring genes from large amounts of filial generations; using the strains comprising the radish cytoplasmic male sterility restoring genes as the female parent and using Zhongshuang No.2, Huashuang No.3, Huyou 17, Zunyou No.1 and Zheyou 758 of double-low winter rapeseed varieties in China as recurrent male parents to hybridize, performing the continuous inbreeding directional selection for six years to breed the cytoplasmic male sterility restoring line of the double-low and high-oil brassica napus rapeseed and radish. The restoring line has been crossed with hybrid varieties. A gap of producing the hybrid varieties by the cytoplasmic male sterility restoring line of the double-low radish in China is filled, and the application prospect is broad.

Description

technical field [0001] The invention relates to the selection and breeding of a restorer line of three-line hybrid rapeseed, in particular to the selection and application of a cytoplasmic male sterile restorer line of Brassica radish. Background technique [0002] At present, the rape CMS with the most practical application value in the world is the Polyma CMS and the Shan 2A CMS. A series of rapeseed hybrids have been successfully selected using the Polyma CMS and the Shan 2A CMS. But these two kinds of sterile lines all have the problem of temperature sensitivity, that is to say, the fertility of these two sterile lines is unstable at low temperature, and both produce a certain amount of fertile pollen, and the sterile lines are easy to self-fertilize. The generated hybrid seeds have more than 10% sterile plants, the purity of the seed production is affected to a certain extent, and the yield of the hybrid cannot fully represent itself. [0003] Nuclear male sterile line...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 刘绚霞董振生刘创社
Owner NORTHWEST A & F UNIV
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