Immunofluorescence chromatography test paper for CRP (C-reaction protein)/SAA (Serum amyloid A protein) quantitative combined detection and preparation method of immunofluorescence chromatography test paper

Abstract

The invention discloses immunofluorescence chromatography test paper for CRP (C-reaction protein)/SAA (Serum amyloid A protein) quantitative combined detection, aiming at providing a test strip capable of realizing quantitative combined detection on the content of CRP/SAA in human peripheral blood and quantitative combined detection on the content of CRP/SAA in venous blood. The immunofluorescence chromatography test paper is technically characterized by comprising a box with a cover, wherein a detection test strip and a blood diluent bottle are arranged in the box, the detection test strip comprises a bottom lining, the bottom lining is provided with a nitrocellulose membrane, one end of the nitrocellulose membrane is connected with a fluorescent microsphere marked antibody fixation pad, the fluorescent microsphere marked antibody fixation pad is connected with a sample pad, the other end of the nitrocellulose membrane is connected with an absorption pad, the fluorescent microsphere marked antibody fixation pad is coated by a CRP monoclonal antibody and an SAA monoclonal antibody, a CRP detection line coated by a CRP monoclonal antibody, an SAA detection line coated by an SAA monoclonal antibody, and a quality control line coated by a goat-anti-mouse IgG polyclonal antibody are arranged on the nitrocellulose membrane in parallel. The immunofluorescence chromatography test paper belongs to the technical field of biological medicines.

Description

technical field [0001] The invention relates to a fluorescent chromatography test paper and a preparation method thereof, in particular to a CRP / SAA quantitative combined detection immunofluorescence chromatography test paper and a preparation method thereof; belonging to the technical field of biomedicine. Background technique [0002] C-reactive protein (C-reaction, CRP) is an acute phase protein synthesized by the liver. It exists in trace amounts in the serum of healthy people. When the body is infected or tissue damage, the content increases rapidly. The recovery of tissue structure and function restores its content to normal levels. At present, CRP, as a sensitive indicator for early detection of systemic inflammatory response, has been widely used in the diagnosis, treatment and prognosis of many clinical diseases. According to the concentration level of CRP in serum, plasma or whole blood, it can effectively judge whether there is infection, the degree of risk of th...

AI Technical Summary

Problems solved by technology

[0005] However, there is currently no CRP / SAA simultaneous quantitative joint detection product on the market, and the simultaneous detection of CRP / SAA concentration in patients can only be detected item by item.

On the one hand, it is time-consuming and labor-intensive. On the other hand, it causes waste of reagents and consumables, which increases the cost of detection and treatment. However, when the body is in an infected state, both CRP and SAA exist in the body in mg / L. detection conditions

[0006] In addition, most of the current rapid detection reagents require a large sample size, which cannot be detected by fingertip blood. It needs to collect venous blood for detection, and blood sample collection requires professional operation, which makes rapid detection test strips very difficult. Difficult to popularize to communities or individuals in diagnosis, physical examination, etc.

Images