Fluorescent microsphere immunity chromatography test paper for detecting food-borne pathogenic microbe
A technology for immunochromatographic test strips and pathogenic microorganisms, which is applied in measurement devices, analytical materials, instruments, etc., can solve the problems of low contrast between color bands and background, difficulty in realizing multiple inspections and joint inspections, and inability to realize quantitative detection, etc. Achieve rapid and accurate qualitative and quantitative detection, rapid detection, and low prices
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Embodiment 1
[0064] Example 1: Preparation of Fluorescent Microsphere Immunochromatographic Test Strips for Detecting Listeria monocytogenes in Beef Stuffing Samples in Farmer's Vegetable Market
[0065] 1. Preparation of chromatography test strips
[0066] 1. Preparation of nitrocellulose membrane;
[0067] Preparation of detection area and quality control area:
[0068] Monocytogenes polyclonal antibody and anti-mouse antibody were coated onto nitrocellulose membrane: with 0.1M PBS pH7.2 (phosphate buffered saline containing 5% sucrose and 0.05% Tween-20 ( Tween-20)) adjusts the concentration of Listeria monocytogenes polyclonal antibody to be 0.5mg / mL, and the resulting solution is sprayed on the membrane as the detection area, and the PBS of 0.01MpH7.2 (which contains 5% sucrose and 0.05 % Tween-20 (Tween-20)) adjusts the concentration of anti-mouse antibody to be 0.5mg / mL, and the solution of gained is sprayed on the film as the quality control area, and the amount of spray film in ...
Embodiment 2
[0079] Embodiment two: the preparation of the fluorescent microsphere immunochromatography test strip of detecting Escherichia coli O157:H7 in the milk sample;
[0080] 1. Preparation of chromatography test strips;
[0081] 1. Preparation of nitrocellulose membrane;
[0082] Preparation of detection area and quality control area: adjust the concentration of Escherichia coli O157:H7 polyclonal antibody to 1.0 mg / mL with 0.05M PBS pH7.2 (containing 5% sucrose and 0.05% Tween-20), and the resulting solution Spray on the membrane as the detection area, adjust the concentration of anti-mouse antibody to 1.0 mg / mL with 0.01M PBS pH7.2 (which contains 5% sucrose and 0.05% Tween-20), and spray the resulting solution on the membrane as the detection area. In the control area, the amount of spray film in the two areas is 0.74μL / cm, the two areas are separated by 5mm, and the quality control area is 2mm away from the end of the nitrocellulose membrane. After drying at 37°C overnight, st...
Embodiment 3
[0093] Example 3: Preparation of fluorescent microsphere immunochromatographic test strips for detecting Staphylococcus aureus and Listeria monocytogenes in pork samples
[0094] 1. Preparation of chromatography test strips
[0095] 1. Preparation of nitrocellulose membrane;
[0096] Detection area preparation: adjust the concentration of Listeria monocytogenes monoclonal antibody to 0.5mg / mL with 0.01M PBS of pH7.2, and adjust the concentration of Staphylococcus aureus monoclonal antibody to 0.8mg / mL, and then the obtained The solution was sprayed on the membrane to form two detection lines, the amount of the sprayed membrane was 0.74 μL / cm, the distance between the two lines was 5mm, and the distance between the absorbent paper was 2mm, after drying at 37°C overnight, it was stored in a dry environment at room temperature for later use.
[0097] 2. Preparation of fluorescent microsphere pads;
[0098] Preparation of the polyclonal antibody against Listeria monocytogenes la...
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