Kit for detecting total polyphenol content in beverage, preparation method of kit and method for detecting total polyphenol content in beverage using kit

A kit and technology of total polyphenols, which are applied in the direction of analysis by chemical reaction of materials and material analysis by observing the influence of chemical indicators, to achieve easy batch detection, shorten detection time, and simple and easy application. Effect

Inactive Publication Date: 2015-11-04
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0013] To sum up, there are great differences in the sensitivity, accuracy, detection complexity and cost of various detection methods. The green detection method is still an urgent requirement of the society and an important research goal

Method used

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  • Kit for detecting total polyphenol content in beverage, preparation method of kit and method for detecting total polyphenol content in beverage using kit
  • Kit for detecting total polyphenol content in beverage, preparation method of kit and method for detecting total polyphenol content in beverage using kit
  • Kit for detecting total polyphenol content in beverage, preparation method of kit and method for detecting total polyphenol content in beverage using kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1: the establishment of Folin-Ciocalteu detection system

[0077] The combined reagents of this embodiment are standard solution a, standard solution b, chromogenic agent, and chromogenic stabilizer;

[0078] 1. The configuration of the reagents used:

[0079] Configuration of standard solution a: Weigh 5 mg of gallic acid standard product with a molecular balance, dissolve it in 50 mL of ultrapure water, vortex to fully dissolve it, and the mass concentration is 0.1 mg / mL.

[0080] Configuration of standard solution b: Weigh 5 mg of tea polyphenol standard with molecular balance, dissolve in 50 mL of ultrapure water, vortex to fully dissolve, and the mass concentration is 0.1 mg / mL.

[0081]The configuration of the chromogen: take 1 part of Folin's phenol reagent and 9 parts of distilled water, mix them by vortexing, and mix evenly, and the volume concentration is 10%.

[0082] Configuration of color stabilizer: 12 mg of sodium carbonate powder was weighed...

Embodiment 2

[0092] Embodiment 2: the establishment of gallic acid standard curve

[0093] Establish a standard curve according to the optimized detection system in Example 1: pipette 0 μL, 20 μL, 40 μL, 60 μL, 80 μL, 100 μL, 120 μL, 140 μL, 160 μL, 180 μL, 200 μL, 220 μL, 240 μL gallic acid standard solution in 13 tubes In the stoppered test tubes, add ultrapure water and 10% folinol reagent to 13 test tubes, and the sampling volumes of the ultrapure water and 10% folinol reagent are 0.8mL and 0.48mL respectively; vortex, mix After evenly standing still for 3 minutes, add 12% sodium carbonate solution to 13 standard tubes, the volume of the sodium carbonate solution is 0.16mL, and finally use ultrapure water to set the volume to 4mL. The absorbance was measured at a wavelength of 696nm, and the entire experimental process should be protected from light. Draw the standard curve with the absorbance value and gallic acid concentration as the vertical and horizontal coordinates respectively,...

Embodiment 3

[0094] Example 3: Evaluation of the Folin-Ciocalteu analytical method

[0095] 1. Repeatability evaluation

[0096] The repeatability evaluation of the analysis method was verified by inter-group experiments. Take 5 parts of 0.16mL 8-fold diluted brand 1 mixed fruit juice samples (commercially available), 5 parts of 0.16mL 8-fold diluted brand 2 green tea samples (commercially available), 5 parts of 0.16mL and brand 3 fruit vinegar samples (commercially available) ), 5 parts of 0.16mL diluted 8-fold brand 4 peach juice samples (commercially available) in 20 test tubes with stoppers, measure the absorbance by the color development system established in Example 1, and calculate the sample according to the regression equation established in Example 2 Contains polyphenol content. Calculate the relative standard deviation and evaluate the precision of the analytical method. The relative standard deviations of the 5 parallel experiments of the 4 samples were all far less than abo...

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Abstract

The invention provides a kit for detecting the total polyphenol content in beverage, a preparation method of the kit and a method for detecting the total polyphenol content in beverage using the kit, and belongs to the field of food nutrition detection. The kit includes a coloring agent, a color stabilizer and a standard substance. According to the invention, an optimized Folio-Ciocalteu method is adopted; a beverage sample develops color after being subjected to a water bath reaction with a combined reagent at 45 DEG C for 0.5 h, and the color reaction time is reduced; the application of the kit to ultraviolet spectrophotometers and semi-automatic biochemical analyzers is simple, easy to operate and high in sensitivity, and a standard curve is not required, so that the detection time is greatly reduced, especially the application to the semi-automatic biochemical analyzers, the detection result can be read visually, and the detection difficulty is reduced; a plurality of beverage samples can be subjected to coloration and detection at the same time, so that the detection is efficient, accurate and fast, and batch detection is facilitated; therefore, the kit and detection method, provided by the invention, have great application prospects in detecting the total polyphenol content in liquid or solid beverage.

Description

technical field [0001] The invention provides a kit for detecting the content of total polyphenols in beverages, a preparation method and a detection method using the kit, and in particular relates to a kit for determining total polyphenols in beverages and a detection method suitable for semi-automatic biochemical analyzers , can be used for the determination of the total polyphenol content of liquid and solid drinks, and belongs to the field of food nutrition testing. Background technique [0002] Plant polyphenols are the general term for polyhydroxy compounds. In a narrow sense, they are tannins or tannins. In a broad sense, they also include small molecular phenolic compounds, such as anthocyanins, catechins, quercetin, and gallic acid. It is widely found in vegetables, fruits, beans, grains and other plants, especially in seed plants. Apples, grapes, lychees, berries and other fruits contain high levels of plant polyphenols. They also exist in tea, beer, fruit wine, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 陈朝银李杰赵声兰葛锋刘迪秋
Owner KUNMING UNIV OF SCI & TECH
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