The invention provides a kit for detecting the total
polyphenol content in beverage, a preparation method of the kit and a method for detecting the total
polyphenol content in beverage using the kit, and belongs to the field of food
nutrition detection. The kit includes a coloring agent, a color stabilizer and a standard substance. According to the invention, an optimized Folio-Ciocalteu method is adopted; a beverage sample develops color after being subjected to a water bath reaction with a combined
reagent at 45 DEG C for 0.5 h, and the
color reaction time is reduced; the application of the kit to
ultraviolet spectrophotometers and semi-automatic biochemical analyzers is simple, easy to operate and high in sensitivity, and a
standard curve is not required, so that the detection time is greatly reduced, especially the application to the semi-automatic biochemical analyzers, the detection result can be read visually, and the detection difficulty is reduced; a plurality of beverage samples can be subjected to coloration and detection at the same time, so that the detection is efficient, accurate and fast, and batch detection is facilitated; therefore, the kit and detection method, provided by the invention, have great application prospects in detecting the total
polyphenol content in liquid or
solid beverage.