Simple and rapid determination method for alcoholic strength of 40-50DEG wine

A technology for rapid determination of alcohol content, applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of unsatisfactory rapid detection development, long detection process, poor stability, etc., and achieve low price, good accuracy Safety and reliability, the effect of less sample volume

Inactive Publication Date: 2018-02-02
黄种山
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The entire detection process is time-consuming, has many steps, and poo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Simple and rapid determination method for alcoholic strength of 40-50DEG wine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 is the making of alcohol content determination standard curve in the simple and quick determination method of alcohol content of 40 to 50 degree wine of the present invention.

[0015] (1) Manual 40-50% wine sample preparation

[0016] Use corresponding pipettes to take 40.0, 42.0, 44.0, 46.0, 48.0 and 50.0ml of absolute ethanol respectively into a 100ml volumetric flask, continuously add distilled water and repeatedly slightly shake and let stand until the volume is finally set to 100ml, and stand at room temperature for 10min. Promptly obtained the artificial 40 to 50 degree liquor samples of different alcohol degrees.

[0017] (2) Manual 40 to 50 degree wine sample pretreatment

[0018] Use a pipette gun to pipette 400ul of the 40-50°C artificial wine sample (30.0°C) to be tested into an empty ordinary microplate plate, first add 50ul of a mixed aqueous solution of 10% by mass volume fraction of urea and 12% by volume volume fraction of formamide As an...

Embodiment 2

[0026] Embodiment 2 is to utilize new method of the present invention to the actual mensuration of certain 10 to 20 degree wine alcoholic strength.

[0027] (1) Pretreatment of wine samples to be tested

[0028] Choose the commercially available whiskey of Maeser (Shandong Wozu Penglai Winery Co., Ltd.), and take two bottles (Messer 1 and Maser 2) for alcohol determination. Use a pipette gun to pipette 400ul of the wine sample (30.0°C) into an empty common microtiter plate, first add 50ul of a mixed aqueous solution of 10% by mass volume fraction of urea and 12% by volume volume fraction of formamide as an initial denaturant, and then Add 50 ul of the short double-stranded DNA fragment mixture system shown in SEQ ID NO: 1-2 (the concentration of each fragment is 10 μmol / l), the total system is 500 ul, mix well and let stand for 5 minutes.

[0029] At the same time, set the control well as a system of 400ul wine sample to be tested plus 50ul initial denaturant plus 50ul distil...

Embodiment 3

[0036] Embodiment 3 is the determination of the alcohol content of the commercially available Niulanshan Erguotou (produced by Niulanshan Distillery of Beijing Shunxin Agricultural Co., Ltd.) by using the new method of the present invention. Lanshan 1 and Niulanshan 2). Operation with reference to Example 2, the results are shown in Table 3. In addition, the density bottle method is used to measure the alcohol content of this wine.

[0037] Table 3 The alcoholic strength numerical value that method of the present invention and density bottle method measure

[0038] Erguotou

[0039] As can be seen from Table 3, the deviations of the alcohol content of two bottles of Niulanshan Erguotou measured by the method of the present invention and the density bottle method are 0.24% and 0.35% respectively, all of which meet the accuracy of alcohol content determination specified by the national standard within 1%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention utilizes the principle that melting can occur to DNA fragments in an ethanol-containing system and cause absorbance change under 260nm wavelength and establishes a new alcoholic strengthdetermination method. Directed at 40-50DEG wine, the invention screens out an appropriate short double-stranded DNA fragment hybrid system shown as SEQ ID NO:1-2, establishes a standard curve equation of the absorbance x and wine sample alcoholic strength y as y=9.1096x+38.38 (y is less than or equal to 50 and greater than or equal to 40). The alcoholic strength determination method provided by the invention is suitable for determination of 40-50DEG wine, has the advantages of good accuracy and reliability, no need for distillation and safety, simple and rapid operation, small wine sample amount, and low price, and is suitable for 40-50DEG wine alcoholic strength determination practice of wineries.

Description

technical field [0001] The invention relates to an ethanol detection method, in particular to a simple and rapid method for measuring the ethanol content of 40-50% wine, that is, the alcohol content. Background technique [0002] Ethanol is the main component of wine, and its content is one of the important indicators of wine quality. It is called alcohol in the national standard, and it usually refers to the number of milliliters of ethanol contained in every 100 ml of wine at 20 °C. Alcohol level is a must-test indicator for alcoholic products. There are currently many determination methods, common ones include alcohol meter method, density bottle method, gas chromatography and near-infrared spectroscopy. Gas chromatography and near-infrared spectroscopy are two methods based on modern analytical technology, which have the advantages of high detection sensitivity, accurate results, and high-throughput measurement, but they also have disadvantages such as expensive equipmen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/33G01N1/38
CPCG01N21/33G01N1/38
Inventor 黄种山黄海霞
Owner 黄种山
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap