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Simple and quick determination method of alcoholic strength of wine with alcoholic strength of 1 to 10 degree

A technology for rapid determination and alcohol content, which is applied in the direction of color/spectral characteristic measurement and analysis through chemical reaction of materials, etc. It can solve the problems of unsatisfactory rapid detection development, long detection process, poor stability, etc., and achieve low price , good accuracy and reliability, the effect of less wine sample

Inactive Publication Date: 2017-12-19
黄海霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The entire detection process is time-consuming, has many steps, and poor stability, which cannot meet the needs of rapid detection development

Method used

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  • Simple and quick determination method of alcoholic strength of wine with alcoholic strength of 1 to 10 degree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 is the making of alcohol content determination standard curve in the simple and quick determination method of alcohol content of 1 to 10 degree wine of the present invention.

[0015] (1) Manual preparation of 1 to 10% wine samples

[0016] Use corresponding pipettes to take 0.0, 1.0, 3.0, 5.0, 7.0, 9.0, 10.0ml of absolute ethanol into a 100ml volumetric flask, add distilled water repeatedly, shake slightly and let it stand, and finally set the volume to 100ml, and let it stand at room temperature After 10 minutes, the artificial 1-10% wine samples with different alcohol levels were obtained. 0.00 is the extreme point, representing the ethanol-free system.

[0017] (2) Manual 1 to 10 degree wine sample pretreatment

[0018] Use a pipette gun to pipette 400ul of the artificial wine sample (30.0°C) of 1 to 10 degrees to be tested into an empty ordinary microplate plate, first add 2% mass volume fraction of urea and 3% volume volume fraction of formamide mix...

Embodiment 2

[0026] Embodiment 2 is to utilize the new method of the present invention to the actual measurement of certain 1 to 10 degree alcoholic strength.

[0027] (1) Pretreatment of wine samples to be tested

[0028] Choose RIO Vodka (Ruiao Weixing, produced by Shanghai Bacchus Liquor Co., Ltd.), a commercially available cocktail, and take two bottles (RIO Vodka 1 and RIO Vodka 2) for alcohol determination. Use a pipette gun to pipette 400ul of the wine sample (30.0°C) into an empty common microtiter plate, first add 50ul of a mixed aqueous solution of 2% by mass volume fraction of urea and 3% by volume volume fraction of formamide as an initial denaturant, and then Add 50 ul of the short double-stranded DNA fragment mixture system shown in SEQ ID NO: 1-2 (the concentration of each fragment is 10 μmol / l), the total system is 500 ul, mix well and let stand for 5 minutes.

[0029] At the same time, set the control well as a system of 400ul wine sample to be tested plus 50ul initial de...

Embodiment 3

[0036] Embodiment 3 is the determination of the alcohol content of commercially available Tsingtao high-concentration beer (produced by Tsingtao Brewery Co., Ltd.) from 1 to 10 degrees by using the new method of the present invention. Take two bottles (Tsingtao Brewery 1 and Tsingtao Brewery 2) in total . Operation with reference to Example 2, the results are shown in Table 3. In addition, the current national standard density bottle method for beer is used to measure the alcohol content of this wine.

[0037] Table 3 The alcoholic strength numerical value that method of the present invention and density bottle method measure

[0038] beer

[0039] As can be seen from Table 3, the deviations of the two bottles of Tsingtao beer alcohol content measured by the method of the present invention and the density bottle method are 0.59% and 0.39% respectively, and all meet the accuracy of the alcohol content determination of the national standard within 1%.

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Abstract

The alcoholic strength is a necessary index for testing an alcohol product, but at present, an accurate, reliable, simple, convenient, fast and economical and practical alcoholic strength determination method is in shortage. A new alcoholic strength determination method is established by utilizing the phenomenon that a DNA (Deoxyribonucleic Acid) fragment is unlinked in an ethanol system so as to cause the change of absorbancy of the DNA fragment under 260nm wave length. According to the method provided by the invention, aiming at wine with the alcoholic strength of 1 to 10 degree, an appropriate short double-chain DNA fragment mixing system is selected as shown in SEQ ID NO:1-2, and a standard curve equation of the absorbancy x and the alcohol strength y of a wine sample, which is that y is equal to 8.8599x-0.5236 (y is larger than or equal to 1 and smaller than or equal to 10) is established. The alcoholic strength determination method provided by the invention is applicable to determining the wine with the alcoholic strength of 1 to 10 degree, has favorable accuracy and reliability, and is safer without the need for distilling, simple, convenient and fast to operate, less in required wine sample amount, low in price, and suitable for determination practice of the alcohol strength of the wine with the alcoholic strength of 1 to 10 degree in a winery.

Description

technical field [0001] The invention relates to an ethanol detection method, in particular to a simple and rapid method for measuring the ethanol content of 1 to 10 degree wine, that is, the alcohol content. Background technique [0002] Ethanol is the main component of wine, and its content is one of the important indicators of wine quality. It is called alcohol in the national standard, and it usually refers to the number of milliliters of ethanol contained in every 100 ml of wine at 20 °C. Alcohol level is a must-test indicator for alcoholic products. There are currently many determination methods, common ones include alcohol meter method, density bottle method, gas chromatography and near-infrared spectroscopy. Gas chromatography and near-infrared spectroscopy are two methods based on modern analytical technology, which have the advantages of high detection sensitivity, accurate results, and high-throughput measurement, but they also have disadvantages such as expensive ...

Claims

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Application Information

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IPC IPC(8): G01N21/75G01N21/33
CPCG01N21/75G01N21/33
Inventor 黄海霞黄种山
Owner 黄海霞
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