Simple and quick determination method of alcoholic strength of wine with alcoholic strength of 1 to 10 degree
A technology for rapid determination and alcohol content, which is applied in the direction of color/spectral characteristic measurement and analysis through chemical reaction of materials, etc. It can solve the problems of unsatisfactory rapid detection development, long detection process, poor stability, etc., and achieve low price , good accuracy and reliability, the effect of less wine sample
Inactive Publication Date: 2017-12-19
黄海霞
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Abstract
The alcoholic strength is a necessary index for testing an alcohol product, but at present, an accurate, reliable, simple, convenient, fast and economical and practical alcoholic strength determination method is in shortage. A new alcoholic strength determination method is established by utilizing the phenomenon that a DNA (Deoxyribonucleic Acid) fragment is unlinked in an ethanol system so as to cause the change of absorbancy of the DNA fragment under 260nm wave length. According to the method provided by the invention, aiming at wine with the alcoholic strength of 1 to 10 degree, an appropriate short double-chain DNA fragment mixing system is selected as shown in SEQ ID NO:1-2, and a standard curve equation of the absorbancy x and the alcohol strength y of a wine sample, which is that y is equal to 8.8599x-0.5236 (y is larger than or equal to 1 and smaller than or equal to 10) is established. The alcoholic strength determination method provided by the invention is applicable to determining the wine with the alcoholic strength of 1 to 10 degree, has favorable accuracy and reliability, and is safer without the need for distilling, simple, convenient and fast to operate, less in required wine sample amount, low in price, and suitable for determination practice of the alcohol strength of the wine with the alcoholic strength of 1 to 10 degree in a winery.
Application Domain
Analysis by subjecting material to chemical reactionColor/spectral properties measurements
Technology Topic
ChemistryStandard curve +6
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