Simple and quick determination method of alcoholic strength of wine with alcoholic strength of 1 to 10 degree

A technology for rapid determination and alcohol content, which is applied in the direction of color/spectral characteristic measurement and analysis through chemical reaction of materials, etc. It can solve the problems of unsatisfactory rapid detection development, long detection process, poor stability, etc., and achieve low price , good accuracy and reliability, the effect of less wine sample

Inactive Publication Date: 2017-12-19
黄海霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The entire detection process is time-consuming, has many steps, and poo

Method used

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  • Simple and quick determination method of alcoholic strength of wine with alcoholic strength of 1 to 10 degree

Examples

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Example Embodiment

[0013] Example 1

[0014] Example 1 is the preparation of a standard curve for the determination of alcohol in the simple and rapid method for determining the alcohol content of 1 to 10 alcoholic liquors of the present invention.

[0015] (1) Manual preparation of wine samples of 1 to 10 degrees

[0016] Use the corresponding pipette to take 0.0, 1.0, 3.0, 5.0, 7.0, 9.0, 10.0ml of absolute ethanol into a 100ml volumetric flask, continuously add distilled water and repeatedly shake and stand, and finally set the volume to 100ml, and let it stand at room temperature In 10 minutes, artificial 1 to 10 alcohol samples with different alcohol levels are obtained. 0.00 is the extreme point, representing the ethanol-free system.

[0017] (2) Manual pre-processing of wine samples from 1 to 10 degrees

[0018] Use a pipette to transfer 400ul of the artificial wine sample (30.0℃) to be tested from 1 to 10 degrees to an empty ordinary ELISA plate, and first add 50ul of a mixed aqueous solution of ...

Example Embodiment

[0025] Example 2

[0026] Example 2 is the actual measurement of the alcohol content of a certain 1 to 10 alcohol by using the new method of the present invention.

[0027] (1) Pre-treatment of wine samples to be tested

[0028] Choose RIO Vodka (Rui Ao Weibo, produced by Shanghai Bacchus Wine Co., Ltd.), a commercially available cocktail, and take two bottles (RIO Vodka 1 and RIO Vodka 2) for alcohol determination. Use a pipette to transfer 400ul of the wine sample (30.0℃) to an empty common ELISA plate. First add 50ul of 2% urea and 3% formamide as the initial denaturant. Add 50ul of the short double-stranded DNA fragment mixing system shown in SEQ ID NO: 1-2 (each fragment concentration is 10μmol / l), then the total system will be 500ul, mix well and let stand for 5min.

[0029] At the same time, set the control hole to 400ul wine sample to be tested plus 50ul initial denaturant plus 50ul distilled water system (the total volume is also 500ul), each wine sample to be tested and the...

Example Embodiment

[0035] Example 3

[0036] Example 3 is to use the new method of the present invention to determine the alcohol content of commercial Qingdao high-concentration beer (produced by Tsingtao Brewery Co., Ltd.), which is 1 to 10 degrees alcohol. A total of two bottles (Tsingtao Beer 1 and Tsingtao Beer 2) are taken. . Refer to the operation in Example 2, and the results are shown in Table 3. In addition, at the same time, the alcohol content of this wine is determined by the density bottle method of the current national standard of beer.

[0037] Table 3 Alcohol values ​​determined by the method of the present invention and the density bottle method

[0038] beer

[0039] It can be seen from Table 3 that the deviation of the alcohol content of the two bottles of Tsingtao Beer measured by the method of the present invention and the measurement result of the density bottle method are 0.59% and 0.39%, respectively, which meets the accuracy of the alcohol content determination stipulated...

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Abstract

The alcoholic strength is a necessary index for testing an alcohol product, but at present, an accurate, reliable, simple, convenient, fast and economical and practical alcoholic strength determination method is in shortage. A new alcoholic strength determination method is established by utilizing the phenomenon that a DNA (Deoxyribonucleic Acid) fragment is unlinked in an ethanol system so as to cause the change of absorbancy of the DNA fragment under 260nm wave length. According to the method provided by the invention, aiming at wine with the alcoholic strength of 1 to 10 degree, an appropriate short double-chain DNA fragment mixing system is selected as shown in SEQ ID NO:1-2, and a standard curve equation of the absorbancy x and the alcohol strength y of a wine sample, which is that y is equal to 8.8599x-0.5236 (y is larger than or equal to 1 and smaller than or equal to 10) is established. The alcoholic strength determination method provided by the invention is applicable to determining the wine with the alcoholic strength of 1 to 10 degree, has favorable accuracy and reliability, and is safer without the need for distilling, simple, convenient and fast to operate, less in required wine sample amount, low in price, and suitable for determination practice of the alcohol strength of the wine with the alcoholic strength of 1 to 10 degree in a winery.

Description

technical field [0001] The invention relates to an ethanol detection method, in particular to a simple and rapid method for measuring the ethanol content of 1 to 10 degree wine, that is, the alcohol content. Background technique [0002] Ethanol is the main component of wine, and its content is one of the important indicators of wine quality. It is called alcohol in the national standard, and it usually refers to the number of milliliters of ethanol contained in every 100 ml of wine at 20 °C. Alcohol level is a must-test indicator for alcoholic products. There are currently many determination methods, common ones include alcohol meter method, density bottle method, gas chromatography and near-infrared spectroscopy. Gas chromatography and near-infrared spectroscopy are two methods based on modern analytical technology, which have the advantages of high detection sensitivity, accurate results, and high-throughput measurement, but they also have disadvantages such as expensive ...

Claims

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Application Information

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IPC IPC(8): G01N21/75G01N21/33
CPCG01N21/75G01N21/33
Inventor 黄海霞黄种山
Owner 黄海霞
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