Method for applying gold nanoparticles mimetic enzyme in biological detection

A gold nanoparticle and biological detection technology, which is applied in the field of gold nanoparticle-simulating enzyme discovery, and achieves the effects of improving detection accuracy, convenient use, and wide application value.

Inactive Publication Date: 2010-05-12
SOUTHEAST UNIV
View PDF0 Cites 44 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Analyzing the application of the above gold nanoparticles in biological detection and sensing, the principle is based on the surface plasmon optical properties of gold nanoparticles (aggregate discoloration, Raman enhancement, etc.), microscopic imaging properties, adsorption and electron tran

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for applying gold nanoparticles mimetic enzyme in biological detection
  • Method for applying gold nanoparticles mimetic enzyme in biological detection
  • Method for applying gold nanoparticles mimetic enzyme in biological detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] Embodiment, gold nanoparticle is used for enzyme-linked immunoassay (ELISA)

[0038] Materials / reagents: 10nm gold nanoparticles, synthesized by the laboratory according to literature methods. 3,3,5,5-Tetramethylbenzidine (TMB) was purchased from Sigma-Aldrich Inc (USA). Goat anti-human IgG (GAHIgG) was purchased from Bethyl Company. Human IgG and bovine serum albumin (BSA) were purchased from Nanjing Kaiji Biotechnology Development Co., Ltd. PBS buffer (0.01M) at pH=7.4, carbonate buffer (0.05M) at pH=9.6, PBS / T (PBS buffer containing 0.1% Tween-20), 0.2M vinegar at pH=4.5 salt buffer. 30%H 2 o 2 , dimethyl sulfoxide (DMSO), sodium chloride, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium carbonate, sodium bicarbonate, sodium acetate, glacial acetic acid, and Tween-20 were purchased from Sinopharm Chemical Reagent Co., Ltd. company. 96-well ELISA plates were purchased from Costar.

[0039] Instruments: DNP-9052 electric heating constant temp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Sizeaaaaaaaaaa
Login to view more

Abstract

A method for applying gold nanoparticles mimetic enzyme in biological detection adopts gold nanoparticles instead of horse radish peroxidase (HRP) in the biological detection. The detection comprises the following steps: coupling the gold nanoparticles and a specific molecular probe to construct a specific nano-probe; performing specific binding between the nano-probe and the corresponding target molecule to be detected; developing with coloring solution containing peroxide and hydrogen-donating substrate, and measuring absorbance or performing microscopic observation so as to realize the qualitative and quantitative detection of the target molecule. The method belongs to the nanometer material and biomedical nanometer technical field. In the method, the size of the used gold nanoparticles mimetic enzyme is 1-1000nm, the gold nanoparticles mimetic enzyme can imitate HRP to catalyze peroxide and hydrogen-donating substrate to perform a color development reaction, and the enzymatic activity increases with the decrease of the size of the gold nanoparticles. The method of the invention uses the gold nanoparticles to label antibody and other biological molecules to build the similar enzyme labeled antibody and other diagnose preparations, thus having extensive application value.

Description

technical field [0001] The invention relates to the discovery of gold nanoparticle-simulating enzymes, and provides a method for using it to replace horseradish peroxidase for biological detection, belonging to the field of nanometer materials and biomedical nanotechnology. Background technique [0002] Gold nanoparticles are a typical representative of metal nanoparticles, which have a wide range of applications in biomedicine. Gold nanoparticles refer to gold materials with a diameter of gold particles between 1-100nm, and are one of the most stable heavy metal nanoparticles. Gold sols dispersed in water, namely colloidal gold, are often used in immunoassays. Gold nanoparticles are mesoscopic particles with special electronic structure and surface plasmon characteristics. Gold nanoparticles can be synthesized by common reagents such as chloroauric acid and sodium citrate or sodium borohydride, and the reaction process is simple, and the diameter of nanoparticles can be c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/543G01N33/554
Inventor 张宇杨婷黄超吴利清赵寅岗马明顾宁
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap