The invention relates to the technical field of biological medical adhesives, and particularly relates to a chitosan cicatrix biological medical adhesive and a preparation method thereof. Each liter of the chitosan cicatrix biological medical adhesive comprises the following raw materials: 5-30 grams of modified chitosan, 30-50 milliliters of glycerol, 0.5-2.5 grams of herba menthae, 0.1-10 grams of lactic acid, 0.5-2.5 grams of ginger extracting solutions, 3-35 grams of viscosity regulating agents and the balance of purified water. The chitosan cicatrix biological medical adhesive disclosed by the invention is complementary in raw material, can effectively reduce the pruritus, relieve the pain, fade the cicatrix color, ensures that the cicatrix is gradually reduced in area and become smooth, is suitable for inhibiting, alleviating and restoring the cicatrice caused by reasons, such as various burns and scalds, traumata, scratches, operations and acne, accelerates the healing of a wound surface, restores the surface cracks of the cicatrice, inhibits the secretion of collagen inside keloid skin, reduces the pruritus, relieves the pain and fades the cicatrix color.