Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Colorimetric detection method for glucose

A detection method, glucose technology, applied in the field of glucose detection, can solve the problems of complex detection process, long detection time, high operation requirements, etc., and achieve the effect of low detection cost, fast detection speed and good reproducibility

Active Publication Date: 2014-04-30
FUZHOU UNIV
View PDF31 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a colorimetric detection method for glucose, which utilizes glucose oxidase to oxidize glucose to generate hydrogen peroxide, and then uses molybdenum disulfide as a catalyst to catalyze the color reaction between hydrogen peroxide and chromogenic agent to detect glucose, which can Solve the problems in the prior art that the operation requirements for glucose detection are high, the detection process is complicated, the detection cost is high, the detection time is long, and the background interference is large. content

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Detection of standard glucose solution: Mix 20 μL of glucose oxidase solution (10 mg / mL) with 180 μL of glucose solutions of different concentrations prepared with acetic acid-sodium acetate buffer (10 mM, pH 3), and react at 30 °C for 30 min, then add 0.05 mL of molybdenum disulfide solution (18 mg / L), 0.05 mL of 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (20 mM) and 0.2 mL of acetic acid-sodium acetate buffer solution (10 mM, pH 1), mix well, and after standing for 30 min, use visual colorimetry for semi-quantitative analysis, or use UV-visible spectrophotometry at 405 nm wavelength Absorbance for quantitative analysis.

[0024] Serum glucose test: take 30 μL serum sample, dilute to 50 μL with water, then add 500 μL Ba(OH) 2 solution (0.11 M), mix well, then add 500 μL ZnSO 4 solution (0.0765 M), after mixing, centrifuge at 3880 rpm for 10 min. Take 200 μL of the supernatant and dilute it 5 times with acetic acid-sodium acetate buffer (10 ...

Embodiment 2

[0026]Detection of standard glucose solution: Mix 20 μL of glucose oxidase solution (10 mg / mL) with 180 μL of glucose solutions of different concentrations prepared with Tris-HCl buffer (10mM, pH 9), react at 60°C for 30 min, Then add 0.05 mL of molybdenum disulfide solution (18 mg / L), 0.05 mL o-phenylenediamine (0.3M) and 0.2 mL Tris-HCl buffer solution (10mM, pH 9), mix well, stand for 30min, and use the purpose Semi-quantitative analysis was performed by visual colorimetry, or quantitative analysis was carried out by measuring the absorbance at a wavelength of 450 nm by UV-visible spectrophotometry.

[0027] Serum glucose test: take 30 μL serum sample, dilute to 50 μL with water, then add 500 μL Ba(OH) 2 solution (0.11 M), mix well, then add 500 μL ZnSO 4 solution (0.0765 M), after mixing, centrifuge at 3880 rpm for 10 min. Take 200 μL of supernatant and dilute it 5 times with Tris-HCl buffer (10mM, pH 9) as a test sample. Other operations are the same as the determinati...

Embodiment 3

[0029] Detection of standard glucose solution: 20 μL of glucose oxidase solution (10 mg / mL) and 180 μL of Tris-HCl buffer (10mM, pH 6.9) were prepared with concentrations of 0 μM, 5 μM, 20 μM, 40 μM, 60 μM, 80 μM, 100 μM, and 150 μM standard glucose solutions were mixed separately, reacted at 37??C for 30 min, and then added 0.05 mL molybdenum disulfide solution (18 mg / L), 0.05 mL 3,3',5,5'-tetra Methylbenzidine solution (12 mM) and 0.2 mL Tris-HCl buffer solution (10 mM, pH 6.9), mix well, place at 30 °C for 30 min, use visual colorimetry for semi-quantitative analysis, or use UV -Visible spectrophotometry is used to measure the absorbance at 652 nm wavelength for quantitative analysis; or add 10 μL of 20% (V / V) sulfuric acid solution to terminate the reaction, use visual colorimetry for semi-quantitative analysis or use UV-Vis spectroscopy The absorbance was measured at a wavelength of 450 nm by photometry for quantitative analysis.

[0030] The results show that with the i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a colorimetric detection method for glucose. The colorimetric detection method for the glucose comprises the steps of pretreating a sample, adding a buffer solution to dilute the sample, mixing the sample with glucose oxidase, performing reaction to generate hydrogen peroxide, then adding a molybdenum disulfide solution, a developing agent and the buffer solution, and measuring the content of the glucose in the sample by a visual colorimetry method or through an ultraviolet-visible light spectrophotometer after mixing reaction is implemented. According to the colorimetric detection method, glucose oxidase is used for oxidizing the glucose to generate the hydrogen peroxide, and molybdenum disulfide serving as a catalyst is used for catalyzing the hydrogen peroxide to generate color development reaction with the developing agent; the glucose concentration is measured in a semi-quantified manner by the visual colorimetry method or is measured in a quantified manner through the ultraviolet-visible light spectrophotometer. According to the colorimetric detection method for the glucose, the problems of high operation requirement on glucose detection, complicated detection process, high detection cost, long detection time, high background interference and the like in the prior art are solved; the method disclosed by the invention is low in cost and easy to operate; the glucose content of the sample can be quickly detected in a visual manner.

Description

technical field [0001] The invention belongs to the technical field of glucose detection, and in particular relates to a colorimetric detection method for glucose. Background technique [0002] In recent years, due to the influence of many factors such as changes in diet structure, increasingly tense life rhythm and less active and more sedentary lifestyle, the incidence of diabetes worldwide has increased rapidly, making diabetes the third serious threat after tumors and cardiovascular diseases Chronic diseases of human health. The detection of glucose content in serum is the main means of clinical examination and monitoring of diabetes. The current national health standard reference method (standard number: WS / T 350-2011) for the detection of serum glucose content is a method using a combination of hexokinase and glucose-6-phosphate dehydrogenase. These natural enzymes have high substrate specificity and catalytic efficiency under mild conditions, but natural enzymes hav...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/78
Inventor 郭良洽林天然
Owner FUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com