Colorimetric detection method for glucose
A detection method, glucose technology, applied in the field of glucose detection, can solve the problems of complex detection process, long detection time, high operation requirements, etc., and achieve the effect of low detection cost, fast detection speed and good reproducibility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0023] Detection of standard glucose solution: Mix 20 μL of glucose oxidase solution (10 mg / mL) with 180 μL of glucose solutions of different concentrations prepared with acetic acid-sodium acetate buffer (10 mM, pH 3), and react at 30 °C for 30 min, then add 0.05 mL of molybdenum disulfide solution (18 mg / L), 0.05 mL of 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (20 mM) and 0.2 mL of acetic acid-sodium acetate buffer solution (10 mM, pH 1), mix well, and after standing for 30 min, use visual colorimetry for semi-quantitative analysis, or use UV-visible spectrophotometry at 405 nm wavelength Absorbance for quantitative analysis.
[0024] Serum glucose test: take 30 μL serum sample, dilute to 50 μL with water, then add 500 μL Ba(OH) 2 solution (0.11 M), mix well, then add 500 μL ZnSO 4 solution (0.0765 M), after mixing, centrifuge at 3880 rpm for 10 min. Take 200 μL of the supernatant and dilute it 5 times with acetic acid-sodium acetate buffer (10 ...
Embodiment 2
[0026]Detection of standard glucose solution: Mix 20 μL of glucose oxidase solution (10 mg / mL) with 180 μL of glucose solutions of different concentrations prepared with Tris-HCl buffer (10mM, pH 9), react at 60°C for 30 min, Then add 0.05 mL of molybdenum disulfide solution (18 mg / L), 0.05 mL o-phenylenediamine (0.3M) and 0.2 mL Tris-HCl buffer solution (10mM, pH 9), mix well, stand for 30min, and use the purpose Semi-quantitative analysis was performed by visual colorimetry, or quantitative analysis was carried out by measuring the absorbance at a wavelength of 450 nm by UV-visible spectrophotometry.
[0027] Serum glucose test: take 30 μL serum sample, dilute to 50 μL with water, then add 500 μL Ba(OH) 2 solution (0.11 M), mix well, then add 500 μL ZnSO 4 solution (0.0765 M), after mixing, centrifuge at 3880 rpm for 10 min. Take 200 μL of supernatant and dilute it 5 times with Tris-HCl buffer (10mM, pH 9) as a test sample. Other operations are the same as the determinati...
Embodiment 3
[0029] Detection of standard glucose solution: 20 μL of glucose oxidase solution (10 mg / mL) and 180 μL of Tris-HCl buffer (10mM, pH 6.9) were prepared with concentrations of 0 μM, 5 μM, 20 μM, 40 μM, 60 μM, 80 μM, 100 μM, and 150 μM standard glucose solutions were mixed separately, reacted at 37??C for 30 min, and then added 0.05 mL molybdenum disulfide solution (18 mg / L), 0.05 mL 3,3',5,5'-tetra Methylbenzidine solution (12 mM) and 0.2 mL Tris-HCl buffer solution (10 mM, pH 6.9), mix well, place at 30 °C for 30 min, use visual colorimetry for semi-quantitative analysis, or use UV -Visible spectrophotometry is used to measure the absorbance at 652 nm wavelength for quantitative analysis; or add 10 μL of 20% (V / V) sulfuric acid solution to terminate the reaction, use visual colorimetry for semi-quantitative analysis or use UV-Vis spectroscopy The absorbance was measured at a wavelength of 450 nm by photometry for quantitative analysis.
[0030] The results show that with the i...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap