Triazole phospho direct competition joint immune absorption analysis technology and its kit

Abstract

The invention relates to a analyzing method for hostathion direct competition enzyme immunity absorption analysis2 and its kit. Compose 4 hapten (the chemical formula)(n=1-5) and it covalency coupling symphysis with protein to be artificial antigen, use the artificial antigen immunity animal to prepare for antibody with idiosyncratic affinity to hostathion and use horseradish peroxide to mark the hapten. Use antibody to surround and cover the plate with microholes, add it into the mixture of the sample (or the marked standard sample of hostathion) and hapten marked by enzyme, hostathion, hapten marked by enzyme and the antibody on the surface of the polystyrene micro holes have competitive bond, then clear away the educt, add substrate and color-developing agent of enzyme the intensity of enzymatic colour reaction is inversely proportional to the content of hostathion in the sample (or the standard sample), in accordance with it, the hosathion direct competition enzyme immunity absorption analysis technique is invented. The technology can be applied to choose relative reagent and material in the kit, for preparing kit of immunity test, and rapid test for the polystyrene remained in agricultural products and environment.

Description

technical field [0001] The invention relates to a triazophos direct competition enzyme-linked immunosorbent assay method and a kit thereof, which are mainly used in the rapid detection of residual triazophos in agricultural products such as grain, fruits and vegetables, tea and the environment. Background technique [0002] Triazophos is a widely used organophosphate insecticide, which is effective against lepidopteran pests (such as stem borers, cotton bollworms, red spiders, aphids, cabbage caterpillars) that harm crops such as rice, cotton, grain, fruit trees, and vegetables. etc.) have a good control effect, and have a remarkable control effect on plant nematodes and pine caterpillars. Treating the soil with triazophos before planting can prevent cutworms and other noctuid pests. [0003] The toxicity classification of triazophos belongs to the poisoning category. There are two main points of toxicity to humans: one is to inhibit the activity of acetylcholinesterase, and...

AI Technical Summary

Problems solved by technology

[0003] The toxicity classification of triazophos belongs to the poisoning category. There are two main points of toxicity to humans: one is to inhibit the activity of acetylcholinesterase, and the other is to destroy the special protein of the central nervous system, that is, the neural target cell enzyme (NTE), which reduces its activity and affects the nervous system. Function

So far, there are no reports on triazophos immunoassay technology and its kit at home and abroad

Images