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Method for determining hydrogen sulfide in blood and urine

A method of determination, hydrogen sulfide technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of incomplete separation of matrix and achieve the effects of large matrix interference, intuitive qualitative and quantitative determination, and low content

Active Publication Date: 2015-08-26
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Further research found that due to the complex matrix of blood and urine, there are effects of color, protein, and fat, and there is a problem of incomplete matrix separation in co-precipitation and liquid-liquid microextraction.

Method used

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  • Method for determining hydrogen sulfide in blood and urine
  • Method for determining hydrogen sulfide in blood and urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Use this method to detect the content of hydrogen sulfide in human urine

[0025] 1. Fe 3 O 4 Preparation of magnetic nanomaterials: 2mol / L FeCl 3 ·7H 2 O 12mL and 1mol / L FeCl 2 ·4H 2 12mL of O was mixed with 120mL of deionized water, under the protection of nitrogen, 50mL of ammonia was added dropwise to make the pH of the system 9, heated in a water bath at 60℃, stirring speed was 400 rpm, constant temperature stirring reaction for 3h, then washed with deionized water to Neutral, vacuum drying at 70°C for 8 hours to produce Fe 3 O 4 Sexual nanomaterials;

[0026] 2. Fe 3 O 4 SiO 2 Preparation of magnetic nanomaterials: the Fe prepared in step 1 3 O 4 Disperse 1.0 g of magnetic nanomaterials in 100 mL deionized water, under the protection of nitrogen, add 1.0 mol / L sodium silicate 10 mL dropwise to Fe 3 O 4 In the nano-dispersion liquid, the pH of the system is set to 6, at 70℃, stirring speed 800rpm, constant temperature stirring reaction for 2h, washing with de...

Embodiment 2

[0028] Example 2: Use this method to detect the content of hydrogen sulfide in human urine

[0029] 1. Fe 3 O 4 Preparation of magnetic nanomaterials: 2mol / L FeCl 3 ·7H 2 O 12mL and 1mol / L FeSO 4 ·7H 2 O 15mL is mixed in 150mL deionized water, under the protection of nitrogen, 50mL ammonia water is added dropwise to make the pH of the system 10, heated in a water bath at 70℃, stirring speed is 600 rpm, constant temperature stirring reaction for 1.5h, then washed with deionized water To neutral, vacuum drying at 40°C for 12h to obtain Fe 3 O 4 Sexual nanomaterials;

[0030] 2. Fe 3 O 4 SiO 2 Preparation of magnetic nanomaterials: the Fe prepared in step 1 3 O 4 Disperse 1.0g of magnetic nanomaterials in 100 mL of deionized water, under the protection of nitrogen, add 10 mL of 1.0mol / L sodium silicate to Fe 3 O 4 In the nano-dispersion liquid, make the pH of the system 6.5, at 75℃, stirring speed 600rpm, constant temperature stirring reaction for 2.5h, wash 4 times with deionized wat...

Embodiment 3

[0032] Example 3: Using the invention to detect the content of hydrogen sulfide in human blood

[0033] 1. Fe 3 O 4 Preparation of magnetic nanomaterials: 2mol / L Fe 2 (SO 4 ) 3 ·9H 2 O 10mL and 1mol / L FeSO 4 ·7H 2 O 12mL was mixed in 150mL deionized water, under the protection of nitrogen, 50mL ammonia was added dropwise to make the pH of the system 11, heated in a water bath at 80℃, stirring speed was 500rpm, constant temperature stirring reaction for 2h, then washed with deionized water to medium It can be dried under vacuum at 50℃ for 10h to prepare magnetic nanomaterials of ferroferric oxide;

[0034] 2. Fe 3 O 4 SiO 2 Preparation of magnetic nanomaterials: the Fe prepared in step 1 3 O 4 Disperse 1.0 g of magnetic nanomaterials in 100 mL deionized water, under the protection of nitrogen, add 1.0 mol / L sodium silicate 10 mL dropwise to Fe 3 O 4 In the nano-dispersion liquid, the pH of the system is set to 7, at 85℃, stirring speed 400rpm, constant temperature stirring reaction ...

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Abstract

The invention discloses a method for determining hydrogen sulfide in blood and urine through combination between dispersive solid-phase microextraction and high performance liquid chromatography. Methylene blue is formed by free H2S, HS- and S2- in serum and the urine, N,N-dimethyl-p-phenylenediamin dihydrochloride and ferric chloride through derivation under the acidic condition, a modified magnetic nanometer material is added to adsorb the methylene blue, the magnetic material and a substrate are separated under the action of an external magnetic field, and interference of macromolecular substances in the blood and the urine to determination is eliminated; the magnetic material is eluted through a little eluent, quantitative determination is conducted on the eluent through the high performance liquid chromatography, and the detection limit can be 0.005 microgram per milliliter. The method has the advantages of being high in accuracy and sensitivity, simple and rapid.

Description

technical field [0001] The invention belongs to the technical field of drug detection in vivo, and in particular relates to a method for detecting hydrogen sulfide in blood and urine, in particular to a method for detecting hydrogen sulfide in blood and urine combined with dispersed solid-phase microextraction and high-performance liquid chromatography. Background technique [0002] In the cardiovascular system, hydrogen sulfide (H 2 S) can open ATP-sensitive potassium ion channels, relax vascular smooth muscle, and lower blood pressure; H in the body 2 The reduction of S production is closely related to the formation of spontaneous hypertension; exogenous administration of H 2 S can effectively protect myocardial ischemic injury. Since it can be produced in the body and has various physiological functions, H 2 S became the third gas signal molecule after NO and CO. There is increasing evidence that endogenous H 2 S is closely related to many diseases. In many clinical...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14
Inventor 杨亚玲赵娇廖文龙
Owner KUNMING UNIV OF SCI & TECH
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