The invention discloses a method for establishing a pre-pregnancy TSH abnormal reference value, and relates to the technical field of maternal and child health care, and the method comprises the following steps: S1, grouping women subjected to pre-pregnancy TSH detection through a big data statistics mode; S2, performing preliminary grouping according to whether the examined women have the pregnancy history or not; S3, performing grouping according to the ages of the women to be examined, adopting cross grouping during age grouping, taking each age as one group, marking the age as n, taking the women of the age n-2, the age n-1, the age n, the age n + 1 and the age n + 2 as one group, and marking the group as an age N group; S4, performing grouping according to different posture characteristics of the examined persons; S5, performing grouping according to different physical conditions of the examinees on the same day. Under the coordination and cooperation effect of big data, samples are expanded as much as possible on the basis of crowds; through the pre-pregnancy detection system, the TSH values of the tested women are compared, an alarm is given for abnormal values, and guidance is provided for research of the pre-pregnancy TSH abnormal values.