The invention discloses a vertical freezing sampling device and its use method. The sampling device includes an operation ship, an automatic control platform, a lifting system, a liquid CO2 supply device, a sampler and an adjustment bracket installed on the sampler, an automatic control platform, a lifting The system and the liquid CO2 supply device are fixed on the work boat in turn, the lifting system is connected with the sampler through cables, and the adjustment bracket is located at the lower part of the sampler. The hollow copper tube probe in the sampler is connected to the liquid CO2 supply device through the liquid CO2 input line. The automatic leveling system in the automatic control platform controls and adjusts the bracket, so that the copper tube probe is vertically inserted into the deposition layer, and the sample is frozen and collected. Compared with the prior art, the present invention can be used for in-situ collection of sediments in deep water and shallow water areas, and the copper tube probe in the sampler is vertically inserted into the sediments, ensuring the success rate of sampling in deep water and higher flow rates.