A method for efficiently isolating endophytic nitrogen-fixing bacteria in corn

A nitrogen-fixing bacteria and corn technology, applied in the field of microorganisms, can solve the problems of not considering the enrichment effect of plant beneficial microorganisms, not considering the adaptability of the nitrogen-fixing bacteria host, and poor application effect, so as to achieve strong operability and avoid mechanical damage. and the effect of decreasing diversity, improving timeliness and screening efficiency
CN113174346BActive Publication Date: 2022-04-15INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Publication Date
2022-04-15

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Abstract

The invention discloses a method for efficiently isolating endophytic nitrogen-fixing bacteria in corn, belonging to the field of microorganisms. The invention utilizes the selective enrichment effect of corn on nitrogen-fixing bacteria under the condition of insufficient nitrogen supply, and uses corn root pressure to quickly obtain corn transporting tissue juice rich in nitrogen-fixing bacteria resources without external power. The two separate cultures of the cultured tissue juice kept the type and activity of microorganisms in the sample to the greatest extent. With the help of the plant's own selection and enrichment mechanism, the invention can quickly screen high-efficiency endogenous nitrogen-fixing bacteria strains that are mutually beneficial and symbiotic with host crops, effectively improving the screening efficiency of nitrogen-fixing bacteria, simple operation, and good timeliness. , Efficient nitrogen-fixing bacteria isolation and cultivation technology.
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Description

technical field

[0001] The invention relates to the field of microorganisms, in particular to a method for efficiently isolating endophytic nitrogen-fixing bacteria in corn. Background technique

[0002] Nitrogen-fixing microorganisms refer to microorganisms that directly convert free nitrogen in the air into nitrogen-containing compounds through life activities. Among them, rhizobia-legume nitrogen fixation is well known, but this process is limited to legumes. How to isolate highly efficient nitrogen-fixing bacteria strains that can co-exist with non-legume crops from the complex soil microbial resource pool, supplement the nitrogen needed for crop growth and development, reduce the use of agricultural chemical fertilizers, maintain healthy crop growth and increase yield, has become a new issue. An important direction of the round of agricultural green revolution.

[0003] The bottleneck limiting the efficient biological nitrogen fixation of non-legume crops is the host ...

Claims

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