A method for efficiently isolating endophytic nitrogen-fixing bacteria in corn

Abstract

The invention discloses a method for efficiently isolating endophytic nitrogen-fixing bacteria in corn, belonging to the field of microorganisms. The invention utilizes the selective enrichment effect of corn on nitrogen-fixing bacteria under the condition of insufficient nitrogen supply, and uses corn root pressure to quickly obtain corn transporting tissue juice rich in nitrogen-fixing bacteria resources without external power. The two separate cultures of the cultured tissue juice kept the type and activity of microorganisms in the sample to the greatest extent. With the help of the plant's own selection and enrichment mechanism, the invention can quickly screen high-efficiency endogenous nitrogen-fixing bacteria strains that are mutually beneficial and symbiotic with host crops, effectively improving the screening efficiency of nitrogen-fixing bacteria, simple operation, and good timeliness. , Efficient nitrogen-fixing bacteria isolation and cultivation technology.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for efficiently isolating endophytic nitrogen-fixing bacteria in corn. Background technique [0002] Nitrogen-fixing microorganisms refer to microorganisms that directly convert free nitrogen in the air into nitrogen-containing compounds through life activities. Among them, rhizobia-legume nitrogen fixation is well known, but this process is limited to legumes. How to isolate highly efficient nitrogen-fixing bacteria strains that can co-exist with non-legume crops from the complex soil microbial resource pool, supplement the nitrogen needed for crop growth and development, reduce the use of agricultural chemical fertilizers, maintain healthy crop growth and increase yield, has become a new issue. An important direction of the round of agricultural green revolution. [0003] The bottleneck limiting the efficient biological nitrogen fixation of non-legume crops is the host ...

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Problems solved by technology

[0004] At present, the isolation and cultivation methods of endophytic nitrogen-fixing bacteria mainly include two types: 1) direct isolation and screening from environmental samples such as soil, because the screening process does not involve living plants, this type of method does not consider the host adaptability of nitrogen-fixing bacteria, resulting in most The obtained strains cannot form a good mutualistic relationship with the host crops in the actual application process, and the application effect is not good; 2) Collect and screen from a certain organ or tissue of the plant. Although the materials screened by this method are plants, they belong to Static sampling from a single part does not consider the enrichment effect of the plant itself on beneficial microorganisms. At the same time, cumbersome operations such as grinding and extraction of solid plant samples are required, and the separation and cultivation cannot be completed in the first place, resulting in poor timeliness and low screening efficiency.

[0005] At present, there is still a lack of a method for efficiently isolating endophytic nitrogen-fixing bacteria in corn

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