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Magnetic molecular imprinting bionic ELISA (enzyme-linked immuno sorbent assay) detecting method of malachite green

A magnetic molecular imprinting and malachite green technology, which can be used in measuring devices, testing food, analyzing materials, etc., can solve the problems of time-consuming, expensive equipment, and affecting the accuracy of test results.

Active Publication Date: 2015-08-12
JIMEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are cumbersome and time-consuming in the sample pretreatment process, and the equipment is expensive and the detection cost is high; not only need to consume a lot of solvent and a lot of time, but also easily cause secondary pollution, affecting the accuracy of the test results

Method used

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  • Magnetic molecular imprinting bionic ELISA (enzyme-linked immuno sorbent assay) detecting method of malachite green
  • Magnetic molecular imprinting bionic ELISA (enzyme-linked immuno sorbent assay) detecting method of malachite green
  • Magnetic molecular imprinting bionic ELISA (enzyme-linked immuno sorbent assay) detecting method of malachite green

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Embodiment Construction

[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0029] Embodiment of the invention

[0030] A magnetic molecular imprinting bionic ELISA detection method for aquatic product malachite green, the specific steps are as follows:

[0031] (1) Fe 3 o 4 Synthesis of polyethylene glycol: Weigh 0.33g polyethylene glycol, add 8mL ultrapure water, sonicate until the polyethylene glycol is completely dissolved, weigh 2.03g FeCl 3 Add to polyethylene glycol solution for ultrasonic dissolution, then weigh 1.5g FeCl ...

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Abstract

The invention discloses a magnetic molecular imprinting bionic ELISA (enzyme-linked immuno sorbent assay) detecting method. The magnetic molecular imprinting bionic ELISA detecting method includes steps of preparing magnetic molecular imprinted polymer and setting up a direct-competition ELISA method. By the detecting method, uniform-particle MG-MMIPs (malachite green-magnetic molecular imprinted polymers) are prepared by an emulsion polymerization method, adsorption performance is investigated, the MG-MMIPs is used as bionic antibody, and the directly-competitive ELISA detecting method is established by means of competitive adsorption to MG antigen and enzyme labeling MG antigen. Under the optimum reaction conditions, the standard curve sensitivity is 20.14 ugL-1, and the minimum detection limit is 0.12 ugL-1 and is lower than detection limit of 2 ppb (ng / g) of a MG rapid-detection card. Meanwhile, the method has high selectivity for MG, and cross reaction rates for two structural analogues (methyl violet and brilliant green) of the MG are 7.4% and 3.9% respectively.

Description

technical field [0001] The invention relates to a relatively large range, in particular to a magnetic molecular imprinting bionic ELISA detection method of malachite green, an aquatic product. Background technique [0002] Malachite Green (Malachite Green, MG) is a triphenylmethane substance, also known as base block green, basic malachite green, malachite green, basic green, Chinese green or aniline green. Green crystalline solid. Malachite green has been used in many industries, such as leather industry, food dyeing industry, pottery industry, printing and dyeing industry, etc. Since MG can play a very good role in the prevention and treatment of aquatic animal diseases, it has been widely used in aquaculture since 1936. Malachite green can also be used as a fungicide, which has a good effect on preventing the growth of mold in fish eggs and the secondary pollution of fungi. However, malachite green has the disadvantages of high toxicity, high residue, high carcinogenic...

Claims

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Application Information

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IPC IPC(8): G01N33/02G01N33/543
Inventor 黄志勇李璐林郑忠
Owner JIMEI UNIV
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