A zinc ion detection fluorescent probe based on purine parent and its preparation method and application
A fluorescent probe and zinc ion technology, applied in the field of fluorescent probes, can solve the problems of complex sample pretreatment, high price, and long measurement time, and achieve good portability and stability, simple method, and short response time.
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Embodiment 1
[0048] The zinc ion fluorescent probe compound based on the purine parent is prepared by the following method:
[0049] 1. Preparation of 8-formyl-4-methylumbelliferone
[0050] In a 100mL round bottom flask, 4-methylumbelliferone (1.06g, 6mmol) and urotropine (1.96g, 14mmol) were added to 15mL of acetic acid, stirred at 70°C for 7h, after the reaction was complete, Cool to room temperature, then add 20% hydrochloric acid aqueous solution (15mL), continue heating reaction at 70°C for 45min, after the reaction is complete, cool the reactant to room temperature, remove the organic solvent by distillation under reduced pressure, PE / EA (v / v, 3 / 1) was eluted, and after the solvent was distilled off under reduced pressure, 8-formyl-4-methylumbelliferone was dried to obtain 8-formyl-4-methylumbelliferone as a light yellow solid (0.541g, yield 44%).
[0051] 2. Preparation of intermediate II (6-chloro-N4-(naphthalene-1-yl)pyrimidine-4,5-diamine)
[0052] In a 100 mL round bottom fla...
Embodiment 2
[0070] The fluorescent probe for zinc ion detection prepared in Example 1 was made into a 1mM probe stock solution with DMSO, each metal ion was configured into a 3mM metal ion stock solution with deionized water, and added to a 3mL blank solution DMSO-H 2 Add 30 μL of probe stock solution and 50 μL of metal ion stock solution to O (v / v=9:1) and detect with fluorescence spectrometer and ultraviolet spectrophotometer. The test shows that the maximum excitation wavelength of the fluorescent probe is 384 nm, the maximum The emission wavelength is 484nm, and the specific test results are as follows:
[0071] Take two cuvettes, add 3mL blank solution DMSO-H respectively 2 Neutralize 30 μL of the probe stock solution with O (v / v=9:1), add 50 μL of the zinc ion stock solution to one of the cuvettes, and do not add the zinc ion stock solution to the other cuvette for UV spectrum testing. Experimental results such as figure 1 As shown, the fluorescent probe compound itself has a stro...
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