Giant salamander active peptide and application

An active peptide, giant salamander technology, applied in the field of giant salamander active peptide, can solve problems such as single function, achieve broad application prospects, promote the proliferation of skin fibroblasts, and improve the effect of immunity

Active Publication Date: 2016-10-26
ZHANGJIAJIE JINCHI ANDRIAS DAVIDIANUS BIOLOGICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the bioactive peptides of giant salamander are prepared from the muscle, skin, viscera, fat, and blood of giant salamander through enzymatic hydrolysis and separation. Active peptides with different functions can be obtained by usi

Method used

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  • Giant salamander active peptide and application
  • Giant salamander active peptide and application
  • Giant salamander active peptide and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Follow the steps below:

[0027] a. Homogenize giant salamander meat, add 1 volume of 0.01M phosphate buffer with pH 7 and a compound enzyme with a weight ratio of 0.1% to the homogenate, and enzymolyze it for 12 hours; the compound enzyme is marine alkaline Protease and papain are mixed according to the activity unit ratio of 7:3;

[0028] b. Take the supernatant by centrifugation, and separate it with a trypsin immobilized ultrafiltration membrane separator prepared by physical adsorption method with a molecular weight cut-off of 4000 Da or less, and take the liquid that passes through the ultrafiltration membrane;

[0029] c. Pass the Sephadex LH-20 molecular sieve chromatography column (15mm×1000mm), collect the chromatographic product in 1ml per tube, and take the 30th~36th tube of chromatographic product for freeze-drying, such as figure 1 Shown: the collected chromatographic product is the absorption peak of OD280nm (peak II);

[0030] d. The freeze-dried prod...

Embodiment 2

[0034] Follow the steps below:

[0035] a. Homogenize giant salamander meat, add 3 times the volume of 0.1M phosphate buffer with pH 8 and a compound enzyme with a weight ratio of 1% to the homogenate, and enzymolyze it for 48 hours; the compound enzyme is marine alkaline Protease and papain are mixed according to the activity unit ratio of 7:3;

[0036] b. Take the supernatant by centrifugation, and separate it with a trypsin immobilized ultrafiltration membrane separator prepared by physical adsorption method with a molecular weight cut-off of 4000 Da or less, and take the liquid that passes through the ultrafiltration membrane;

[0037] c. Pass the Sephadex LH-20 molecular sieve chromatography column (15mm×1000mm), collect the chromatographic product in 1ml per tube, and take the 30th~36th tube of chromatographic product for freeze-drying, such as figure 1 Shown: the collected chromatographic product is the absorption peak of OD280nm (peak II);

[0038] d. The freeze-dri...

Embodiment 3

[0042] Follow the steps below:

[0043] a. Homogenize giant salamander meat, add 2 times volume of 0.1M phosphate buffer with pH 8 and compound enzyme with a weight ratio of 0.5% to the homogenate, and hydrolyze for 34 hours; the compound enzyme is marine alkaline Protease and papain are mixed according to the activity unit ratio of 7:3;

[0044] b. Take the supernatant by centrifugation, and separate it with a trypsin immobilized ultrafiltration membrane separator prepared by physical adsorption method with a molecular weight cut-off of 4000 Da or less, and take the liquid that passes through the ultrafiltration membrane;

[0045]c. Pass the Sephadex LH-20 molecular sieve chromatography column (15mm×1000mm), collect the chromatography product in 1ml per tube, and take the 30th~36th tube of chromatography product for freeze-drying; figure 1 Shown: the collected chromatographic product is the absorption peak of OD280nm (peak II);

[0046] d. The freeze-dried product obtained...

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PUM

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Abstract

The invention discloses a giant salamander active peptide and an application. The giant salamander active peptide is prepared by the following steps: conducting enzymatic hydrolysis on giant salamander meat which serves as a raw material by virtue of a complex enzyme of marine alkaline protease and papain; separating an enzymatic hydrolysate by virtue of a trypsin immobilized ultra-filtration membrane separator; and then conducting separation and purification by virtue of Sephadex LH-20 molecular sieve chromatography and high performance liquid chromatography, so that the giant salamander active peptide is obtained. The giant salamander active peptide is capable of effectively scavenging free radicals and boosting body immunity, and meanwhile, the active peptide can also promote proliferation of skin fibroblasts; therefore, the giant salamander active peptide has a broad application prospect in the fields of food, medicines and cosmetics.

Description

technical field [0001] The invention belongs to giant salamander products, and in particular relates to a giant salamander active peptide capable of scavenging free radicals, improving body immunity and promoting the growth of skin fibroblasts and its use. Background technique [0002] Studies have shown that the muscles, skin, viscera, fat, and blood of giant salamanders are rich in protein, amino acids, fatty acids, and trace elements. Deep processing of giant salamander proteins can obtain a series of bioactive peptides of giant salamanders that are beneficial to human life activities. Potential source of health food and medicine. At present, most of the bioactive peptides of giant salamander are prepared from the muscle, skin, viscera, fat, and blood of giant salamander through enzymatic hydrolysis and separation. Active peptides with different functions can be obtained by using different separation techniques, but most of them have single functions. So far, there is n...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
CPCC07K1/16C07K1/34C07K1/36C12P21/06
Inventor 李伟佟长青
Owner ZHANGJIAJIE JINCHI ANDRIAS DAVIDIANUS BIOLOGICAL SCI
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