Method for simultaneously extracting soybean peptide and soybean oligosaccharide from aqueous phase produced through aqueous enzymatic method
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A technology for simultaneous extraction of soybean oligosaccharides, applied in the direction of oil/fat production, fermentation, and fat production
Active Publication Date: 2014-02-19
SHANDONG WONDERFUL IND GROUP
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[0006] So far, there is no process for synchronously extracting soybean oil, soybean peptides and soybean oligosaccharides from the aqueous phase of the aqueous enzymatic method, and it is the first tim
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Embodiment 1
[0032] Example 1 Screening experiment of ultrasonic treatment process condition parameters
[0033] 1 Materials and methods
[0034] 1.1 Materials and reagents
[0035]
[0036] 1.2 Main instruments and equipment
[0037]
[0038] 1.3 Experimental method
[0039] 1.3.1 Determination of soybean components
[0040] Determination of moisture: GB304-87; Determination of crude fat: Soxhlet extraction method in GB5512-85; Determination of crude protein: GB6432-94 standard method; Determination of ash: GB5009.4-85.
[0041] 1.3.2 Process flow (see figure 1 )
[0042] 1.3.3 Determination of soybean polypeptide yield: Kjeldahl method.
[0043]
[0044] In the formula ——Soluble nitrogen in 10%TCA, mg
[0045] ——total nitrogen in raw soybean, mg
[0046] 1.3.4 Determination of extraction rate of soybean oligosaccharides:
[0047]
[0048] 2 Results and Discussion
[0049] 2.1 Effect of single factor condition of ultrasonic treatment process on the yield of soybe...
Embodiment 2
[0079] Example 2 Screening experiment of alcohol precipitation treatment process condition parameters
[0087] 1.3.2 Determination of extraction rate of soybean oligosaccharides:
[0088]
[0089] 2 Results and Discussion
[0090] 2.1 Effect of alcohol precipitation treatment on the yield of soybean oligosaccharides
[0091] 2.1.1 Effect of ethanol concentration on yield of soybean oligosaccharides
[0092] Under the conditions of ethanol extraction time of 90min and ethanol extraction temperature of 40°C, the influence of ethanol concentration on the yield of soybean oligosaccharides was investi...
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Abstract
The invention discloses a method for simultaneously extracting soybean peptide and soybean oligosaccharide from an aqueous phase produced through the aqueous enzymatic method and belongs to the technology of gain and oil bioprocessing. The method comprises the following steps: (1) soybeans are extruded and puffed, and then mixed with water for enzymolysis, and centrifugal separation is performed after enzymolysis, so that free oil, an emulsion, a hydrolysate and residues are obtained; (2) the emulsion, the hydrolysate and the residues are subjected to ultrasonic treatment to obtain a mixed liquor, two types of alkaline protease are added into the mixed liquor for step-by-step enzymolysis, centrifugal separation is performed after enzymolysis, so that free oil, an aqueous phase waste liquor and residues are obtained; (3) the acid deposition is performed on the aqueous phase waste liquor and then centrifugal separation is performed on the aqueous phase waste liquor to obtain sediment and aqueous phase mixture, and the sediment is subjected to vacuum concentration and spray drying to obtain soybean peptide; nanofiltration and ethanol precipitation are performed on the aqueous phase mixture to obtain sediment, and the sediment is subjected to decolorization and desalination treatment, and then subjected to vacuum concentration and spray drying to obtain the soybean oligosaccharide. According to the method, the aqueous phase mixed system formed during oil production through the aqueous enzymatic method is fully utilized, so that the waste is reduced, and the soybean peptide and the soybean oligosaccharide can be simultaneously obtained; the method has an excellent application prospect.
Description
technical field [0001] The invention belongs to the biological processing technology of grain and oil, and mainly relates to a method for synchronously extracting soybean polypeptide and soybean oligosaccharide from the water phase of an aqueous enzymatic method. Background technique [0002] The water enzymatic oil extraction technology is based on mechanical crushing, using enzymes to act on the oil, so that the oil is easily released from the oil, using the difference in affinity between non-oil components (protein and carbohydrates) for oil and water, and using oil and water Separation of oil and non-oil components due to the difference in specific gravity. As a new "safe, green and environment-friendly" oil extraction technology, it has been paid more and more attention by many scholars at home and abroad. It can efficiently recover other valuable components (protein and carbohydrates, etc.) in the oil while extracting the oil, which is called "a technology for the ful...
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