Method for improving enzymolysis efficiency of crude heparin sodium extraction technology

Abstract

The invention relates to a method for improving enzymolysis efficiency of a crude heparin sodium extraction technology. The method can effectively improve enzymolysis efficiency, decompose impurity proteins, improve crude product quality, improve a yield and increase economic benefits. The method comprises the following steps of 1, dissolution: preparing mucous membrane water, 2, acid protease catalysis: heating the mucous membrane water, adding acid protease into the mucous membrane water based on the number of small intestines of the pig, adjusting a pH, adding metal ions into the mixed solution based on the number of small intestines of the pig, adjusting salinity by sodium chloride, and carrying out a thermal insulation reaction process, 3, alkaline protease catalysis: carrying out heating, adding two alkaline proteases into the reaction produce based on the number of small intestines of the pig, adding a pH value and salinity, carrying out a thermal insulation reaction process, carrying out heating and carrying out a thermal insulation reaction process, and 4, filtration on the enzymolysis mother liquor by a combined filter cloth, introduction of the filtered enzymolysis mother liquor into an adsorption tank, and follow-up processes.

Description

technical field [0001] The invention relates to a process for preparing crude heparin sodium, in particular to a method for improving the enzymatic hydrolysis efficiency of the crude heparin sodium extraction process. Background technique [0002] Heparin sodium is a commonly used anticoagulant drug in clinical practice. It is a mucopolysaccharide mainly extracted from pig small intestinal mucosa, and has significant anticoagulant and antithrombotic effects. The salting-out extraction method is a commonly used method for extracting heparin from the small intestinal mucosa in China. The main process is to separate the heparin in the small intestinal mucosa from the protein complex with salt in an alkaline environment, and then use a strong basic anion exchange resin to extract it from the protein complex. The heparin anion is adsorbed in the liquid, and then the heparin anion is eluted with a certain concentration of saline solution, and the crude heparin can be obtained afte...

AI Technical Summary

Problems solved by technology

The salting-out extraction method is a commonly used method for extracting heparin from the pig small intestine mucosa in China. The main process is to separate the heparin in the small intestine mucosa from the protein complex with salt in an alkaline environment, and then use a strong basic anion exchange resin to extract the heparin from the protein complex. The heparin anion is adsorbed in the liquid, and then the heparin anion is eluted with a certain concentration of saline solution, and the crude heparin can be obtained after ethanol precipitation and drying; but this treatment method leads to many impurities, especially dermatin, and the yield is low

Another extraction method uses alkaline protease for single enzymatic hydrolysis, which cannot handle acidic proteins, resulting in increased impurities and lower yields