Preparation method of protein short peptide chelated calcium

A protein and chelated calcium technology, which is applied in the field of chelated calcium, can solve the problems of high production cost, low ratio, and reduced water solubility, and achieve the effects of high production safety, reduced production cost, and improved water solubility

Active Publication Date: 2013-08-07
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the existing disclosed protein peptide chelated calcium preparation methods, all include the preparation method of protein peptide, but there are the following deficiencies: one is that the influence of the molecular weight of the peptide on the properties of chelated calcium is not considered, such as water solubility, in the protein peptide In the preparation of chelated calcium, after the peptide with large molecular weight forms chelated calcium, its water solubility decreases and precipitation occurs, which will affect the absorption of chelated calcium in the body. At present, in the existing protein peptide preparation methods, The proportion of protein peptides, especially short peptides such as 2-peptides and 3-peptides is low, and the prop

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A preparation method of protein short peptide chelated calcium is characterized in that the specific steps are as follows: (1) Freshwater silver carp skin is washed, broken, mixed with water at a ratio of 1:15, and the mixture is sent to a colloid mill for micronization Process to fully disperse the raw protein, adjust the protein concentration at 5%, and sterilize the finely ground and uniformly dispersed raw material liquid at 85°C for 60 seconds, and cool it to 50°C with a plate cooler, then add the protein in the raw material liquid 3.0% of the mass was hydrolyzed by adding alkaline protease, the enzymatic hydrolysis conditions were 50° C., the initial pH was 9.0, and the time was 4 hours.

[0041] (2) The hydrolyzate after the first hydrolysis in step (1) is separated through decanter centrifugation and plate-and-frame filtration to separate the unhydrolyzed solid phase part and the hydrolyzate liquid phase part, and collect it respectively; The liquid phase of the...

Embodiment 2

[0047] A preparation method for protein short peptide chelated calcium is characterized in that the specific steps are as follows:

[0048] (1) Food-grade soybean protein isolate is washed and crushed and mixed with water at a weight ratio of 1:15, and the mixture is sent to a colloid mill for micronization treatment so that the raw protein is fully dispersed and evenly formed to form a raw material solution with a protein concentration of 6%. Sterilize at 121°C for 2 seconds, cool down to 45°C with a plate cooler, and then add papain for the first hydrolysis. The conditions for the first hydrolysis are: temperature 50°C, pH 6, time 6 hours, proteolysis The mass ratio of enzyme to raw protein is 4%;

[0049] (2) The hydrolyzate after the first hydrolysis in step (1) is separated by centrifugation in a sedimentation centrifuge and plate-and-frame filtration to separate the unhydrolyzed solid phase part and the liquid phase part of the hydrolyzed liquid, and collect them respect...

Embodiment 3

[0056] (1) Mix food-grade egg white protein powder and water at a weight ratio of 1:12, and send the mixture into a colloid mill for micronization treatment so that the raw protein is fully dispersed and uniformly formed to form a raw material solution with a protein concentration of 8%. After high-temperature sterilization for 5 seconds, cool to 50°C, then add neutral protease for the first hydrolysis, the conditions for the first hydrolysis are: temperature 50°C, pH 7, time 6 hours, the mass ratio of neutral protease to raw protein is 4%;

[0057] (2) The hydrolyzate after the first hydrolysis in step (1) is centrifuged twice continuously through a decanter centrifuge-tubular centrifuge, and the plate and frame filter is separated to separate the unhydrolyzed solid phase part and the hydrolyzed liquid phase part , and collect them separately; then the liquid phase of the collected hydrolyzate is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off o...

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Abstract

The invention provides a preparation method of protein short peptide chelated calcium. The method includes the steps of: refinement treatment and sterilization of mixed raw material containing protein and water, a first hydrolysis by adding proteolytic enzyme, ultrafiltration of the liquid portion separated after the first hydrolysis using a ultrafilter membrane whose retention molecular weight is in the range of 1000-6000 Da, mixing of the macro-molecule peptide liquid which does not permeate the ultrafilter membrane and the solid phase portion separated by the first hydrolysis, a second hydrolysis by adding proteolytic enzyme, purification of micromolecule oligopeptide liquid after twice hydrolysis ultrafiltrations, chelating by adding calcium hydrate or calcium chloride, complete reaction of quantitive sodium carbonate when the pH valve is neutral, filtering for removing deposition, desalination by the nanofiltration membrane, sterilization, condensation and obtaining of the protein short peptide chelated calcium product. The preparation method can substantially improve the protein short peptide obtaining rate and the raw material protein utilization rate, and reduce production cost, so the obtained protein short peptide chelated calcium product has a good quality and high production safety.

Description

technical field [0001] The invention relates to a chelated calcium, in particular to a preparation method of a protein short peptide chelated calcium. Background technique [0002] Calcium is one of the most abundant mineral elements in the human body, and it is also the most active element in the body. It has a variety of important physiological functions. It is an essential component for maintaining the normal excitation of nerves and muscles, and it is also necessary for ensuring bone growth and maintaining bone hardness. substance. Then, calcium deficiency belongs to global health. According to the results of the latest "Nutrition and Health Status Survey of Chinese Residents", about 97% of the population in my country is deficient in calcium. The national average per person per day is only 391mg. Calcium deficiency is relatively common among children, women and the elderly. According to data, the average prevalence of rickets in children under 3 years old in some areas...

Claims

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Application Information

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IPC IPC(8): A23L1/304A23L1/305
Inventor 刘良忠刘闪王梦颖
Owner WUHAN POLYTECHNIC UNIVERSITY
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