Extraction method of collagen polypeptide and active calcium in walleye pollock bone
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A collagen peptide and extraction method technology, applied in chemical instruments and methods, specific peptides, connective tissue peptides, etc., can solve problems such as poor calcium supplementation effect, achieve high-value utilization, ensure extraction rate, and improve utilization rate Effect
Active Publication Date: 2016-10-26
SHANDONG ORIENTAL OCEAN SCI TECH
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[0004] Fish bones contain a lot of calcium, but the calcium in fish bones exists in the form of hydroxyapatite and calcium phosphate, and the effect of calcium supplementation is not good
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[0030] (2) Preparation of collagen polypeptide:
[0031] (1) After soaking the fish bones obtained by the above pretreatment with NaOH solution (concentration 0.5mol / L, soaking time 2 hours), high temperature and high pressure treatment (treatment under the action of high pressure water steaming at 125°C for 40min), and crushing to below 40 mesh, Get fish bone meal.
[0032] (2) Extraction of collagen: extract the above-mentioned fish bone powder with hot water at 85-90° C. (extraction time is 4 hours), and suction filter to obtain collagen solution and bone residue.
[0033] (3) High-voltage pulsed electric field treatment: See Table 2 for the experimental parameters (electric field strength, pulse frequency, and pulse time).
[0034] (4) Enzymolysis: after the above treatment, enzymolysis with complex collagenase and then enzymolysis with flavor protease to improve the flavor. The dosage of compound collagenase is 0.2% (200,000 u / ml), the reaction temperature is 55°C, the ...
Embodiment 1
[0058] The extraction of collagen polypeptide and active calcium in pollock fish bone of embodiment 1
[0059] Proceed as follows:
[0060] (1) Pretreatment of raw materials:
[0061] (1) Acid treatment: 5 kg of pollock fillets after harvesting were soaked in 0.3% HCl solution for 60 minutes, and washed with running water until the pH was 6-7.
[0062] (2) Removal of attached fish meat on the fish bone: add water to the pollock fish steak after the above-mentioned acid treatment, and the ratio of solid to liquid is 1:1, and then add 0.1% animal protease (200,000 u / ml) for enzymolysis, The reaction temperature is 50° C., the reaction pH is 6.5, and the reaction time is 3.5 h.
[0063] (3) Filtration: the product obtained after the above enzymatic hydrolysis was filtered, and the filtrate was fish meat polypeptide to obtain 815 g of fish bones, which were washed for later use.
[0064] (2) Preparation of collagen polypeptide:
[0065] (1) Alkali treatment: immerse the pretre...
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Abstract
The invention discloses an extraction method of collagen polypeptide and active calcium in walleye pollock bone. The method comprises the following steps: (I) pretreating the raw material; (II) preparing bone collagen polypeptide through the substeps of (1) soaking fish bone in a NaOH solution and then carrying out high-temperature and high-pressure treatment, (2) extracting the bone collagen, (3) carrying out treatment via a high-voltage pulsed electric field, and (4) carrying out enzymolysis by firstly adding composite collagen protease for enzymolysis, then adding flavor protease for enzymolysis, decolorizing and deordorizing, carrying out spray drying to obtain the collagen polypeptide; and (III) preparing active calcium in fish bone through the substeps of (1) extracting tartaric acid, (2) extracting tartaric acid again, and (3) combining the extracted tartaric acids to prepare the active calcium in the fish bone. Through the extraction method, the disadvantages of thick fishy smell, bitter taste and unfavorable peculiar smells of the marine source collagen polypeptide can be overcome; the collagen polypeptide of the walleye pollock bone which is free of pungent smell, delicious and moderate in taste can be prepared; meanwhile, the bone dreg can be economically and efficiently used for preparing active tartaric acid chelated calcium; the utilization rate of the walleye pollock bone is increased; the high-value utilization is achieved.
Description
technical field [0001] The invention relates to a method for extracting collagen polypeptide and active calcium in pollock fish bones, and belongs to the technical field of high-value development of fish bones. Background technique [0002] Cod is one of the important fishing objects of my country's distant water fishery, with an annual catch of 100,000 tons. However, the processing and production of marine fish will produce a large amount of waste, of which fish bones account for about 15% of the overall weight of the fish, which are usually discarded directly during production, or only used as feed and fertilizer, resulting in a huge waste of resources. The main components of fish bone are ash, protein, water and fat, and rich in calcium, among which the content of collagen is high. It is a good resource for the development of fish bone collagen peptides and related products, and is of great significance to the comprehensive utilization of aquatic products and environmenta...
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