Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for co-production of collagen calcium and polypeptide in chondroitin sulfate production

A chondroitin sulfate and production process technology, applied in the field of biomedicine, can solve the problems of high content of inorganic salts and destroy the activity of collagen peptides, and achieve the effects of mild production conditions, energy saving and economic value improvement.

Inactive Publication Date: 2015-09-16
JIAXING NUDIKON LIFE SCI
View PDF4 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chinese patent 201310027690.5 discloses a method for producing chondroitin sulfate and co-producing peptone, collagen peptide, and cartilage calcium powder. temperature, destroys the activity of collagen peptides
In addition, in the production process of this patent, the content of inorganic salts in the collagen peptide solution is high, and the desalination by nanofiltration requires repeated water addition, which will generate a large amount of waste water

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Crumble 300g of shark fin bone into coin size, add 1L of 0.5mol / l hydrochloric acid solution, soak for 6 hours, take out the cartilage, adjust the pH value of the solution to 7.0 with 0.5Mol / L calcium hydroxide solution, filter to remove a small part of impurities and After precipitation, add high-concentration alcohol 3 times the volume of the solution to precipitate, wash twice with alcohol, spin dry by centrifugation, and dry in an oven at 60°C to obtain 21.5g of collagen chelated calcium product. Add 1.5L of water to the soaked cartilage, adjust its pH to 8.5 with 0.5mol / L sodium hydroxide solution, add 1.5% neutral protease and 0.75% flavor protease of cartilage dry weight , enzymatically hydrolyzed at 45°C for 6 hours, then raised the temperature to 85°C to inactivate the enzyme for 30 minutes, filtered to obtain the supernatant and bone residue, and weighed about 21g of the bone residue after washing with water and drying. The supernatant was separated by ultrafi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a process for co-production of collagen calcium and polypeptide in chondroitin sulfate production. The production process includes: conducting coarse crushing on shark cartilage to a massive size, performing soaking in an acid solution and then conducting filtering, subjecting the filtrate to calcification treatment and alcohol precipitation, conducting secondary washing with alcohol, and then carrying out spin-drying and drying to obtain collagen chelated calcium; subjecting the soaked cartilage to enzymolysis and filtration to obtain a chondroitin sulfate and collagen polypeptide aqueous solution and bone dreg, subjecting the aqueous solution to ultrafiltration separation to obtain a chondroitin sulfate concentrated solution, and performing alcohol precipitation to obtain chondroitin sulfate; and concentrating the ultrafiltrate, and then carrying out spray drying, thus obtaining collagen polypeptide. On the basis of co-production of chondroitin sulfate and collagen polypeptide, the invention also produces another high benefit product at the same time, i.e. collagen chelated calcium, makes full use of the resources in cartilage, performs high value utilization of bone calcium powder in treatment process, and improves the economic value.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to a production process for co-producing collagen chelated calcium and small molecule polypeptides in the production process of chondroitin sulfate. Background technique: [0002] Chinese patent 201310027690.5 discloses a method for producing chondroitin sulfate and co-producing peptone, collagen peptide, and cartilage calcium powder. Temperature destroys the activity of collagen peptides. In addition, in the production process of this patent, the inorganic salt content in the collagen peptide solution is high, and the nanofiltration desalination needs repeated water addition, which will generate a large amount of waste water. [0003] At present, the by-products co-produced in the chondroitin sulfate production industry are mostly collagen polypeptide, peptone and bone calcium powder, and among the by-products, only collagen polypeptide is the most valuable. Therefore, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C08B37/08C07K14/78C07K1/34
Inventor 朱美红钟名国
Owner JIAXING NUDIKON LIFE SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com