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Kit for detecting fungus (1-3)-beta-D glucan in human body fluid and application method of kit

A detection kit, glucan technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of complicated operation, external bacterial contamination, and many color reagents, etc., and achieve high specificity and detection method. easy effect

Active Publication Date: 2013-01-09
湛江博康海洋生物有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

The problem with this method is that it requires a large number of chromogenic reagents, the operation is cumbersome, and external bacterial contamination is likely to intervene in the intermediate process, which affects the detection results.

Method used

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  • Kit for detecting fungus (1-3)-beta-D glucan in human body fluid and application method of kit
  • Kit for detecting fungus (1-3)-beta-D glucan in human body fluid and application method of kit
  • Kit for detecting fungus (1-3)-beta-D glucan in human body fluid and application method of kit

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Embodiment Construction

[0024] The present invention will be further described below by taking blood (1,3)-β-D glucan detection application as an example in conjunction with the accompanying drawings.

[0025] Manufacture of human body fluid fungal (1,3)-β-D-glucan detection kit:

[0026] 1. The composition of the human body fluid fungal (1,3)-β-D-glucan detection kit includes: dynamic turbidity method reaction main agent, body fluid treatment agent No. Ⅰ (body fluid treatment agent), real No. Ⅱ diluent ( Alkaline diluent), true No. Ⅲ diluent (acidic diluent), (1,3)-β-D-glucan quality control substance, bacterial endotoxin test water, freeze-dried serum.

[0027] 2. The preparation process of the main agent of the dynamic turbidity method is mainly through the extraction and preparation of the limulus blood cell lysate by using anticoagulant, cell lysate and activator, and specifically includes the following steps:

[0028] 2.1 Blood collection: Fresh live horseshoe crabs were sterilized with alcoho...

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Abstract

The invention provides a kit for detecting a fungus (1-3)-beta-D glucan in human body fluid. The kit can be industrially produced, and is accurate and high in speed and specificity. The kit is characterized by comprising a main reactant, a body fluid treating agent, an alkaline diluent, an acidic diluent, sterile water, lyophilized serum and a (1-3)-beta-D glucan quality control substance, wherein the main reactant contains a tachypleus tridentatus blood cell G factor, coagulating zymogen and an activator. A detection method is convenient, rapid, safe and high in specificity, the (1-3)-beta-D glucan in body fluid samples of 10 persons can be detected within 80 minutes, a discrimination coincidence rate reaches 100 percent, and the fungus (1-3)-beta-D glucan and bacterial endotoxin can be discriminated within the same reaction time.

Description

technical field [0001] The invention relates to a human body fluid fungus (1,3)-β-D glucan detection kit and an application method thereof. Background technique [0002] In recent years, the prevalence of clinical invasive fungal infections (IFI) has increased significantly. IFI is also increasingly becoming one of the serious complications and important causes of death in bone marrow and organ transplant recipients, malignant hematological diseases and malignant tumor patients receiving chemotherapy, AIDS and other critically ill patients. The key to the success or failure of the treatment of deep fungal infections lies in early diagnosis. When fungi enter human blood or deep tissues, (1,3)-β-D glucan can be released from the cell wall after phagocytosis, digestion and metabolism by phagocytes β-D-glucan content in blood or other body fluids is increased, and its presence in body fluids can be largely regarded as a sign of IFI (deep fungal infection). The commonly used cl...

Claims

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Application Information

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IPC IPC(8): G01N33/66G01N33/531G01N21/59
Inventor 莫水晶莫世文莫世君
Owner 湛江博康海洋生物有限公司
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