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Method for purifying hyaluronic acid by pre-laying filter aid

A technology of hyaluronic acid and filter aid, which is applied in chemical instruments and methods, separation methods, filtration and separation, etc., can solve the problems of reducing the adsorption and filtering effect of diatomite, reducing the light transmittance of the liquid medicine, and not involving them. The effect of reducing the content of bacterial endotoxin, increasing the effect of filtering and adsorption, and reducing the production cost

Active Publication Date: 2011-01-05
SHANGHAI JINGFENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] This patent has the following disadvantages: the process does not involve the steps of activated carbon adsorption, diatomaceous earth filtration, etc., and the final product obtained has no test data, which cannot explain the purification effect of the process in the patent
[0012] 2. Diatomite is directly added to the liquid medicine. On the one hand, diatomite is prone to sedimentation. On the other hand, the probability of diatomite contacting impurities and bacterial endotoxins in the liquid medicine will be greatly reduced, thereby reducing the adsorption and filtration of diatomite. Effect
[0013] 3. In the initial stage of decarbonization and filtration, the light transmittance of the filtered medicinal solution is reduced due to the lack of an effective filter layer

Method used

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  • Method for purifying hyaluronic acid by pre-laying filter aid
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  • Method for purifying hyaluronic acid by pre-laying filter aid

Examples

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Embodiment 1

[0037] Prepare sodium hyaluronate by the existing extraction method, take 16.5--33 grams of diatomite, add 4L of water to moisten it, and stir evenly to obtain a suspension of diatomite. The electric mixer produced by Shanghai Meiyingpu is used for stirring, and the stirring paddle is in the form of propulsion.

[0038] In the stirring state, use the peristaltic pump (model WL600B-YZ1515 / 2.4) produced by Changzhou Purui Fluid to circulate and filter the suspension of diatomite and pre-spread it on the titanium rod of the titanium rod filter, so that the diatomite is in the A pre-laid filter layer is pre-laid on the titanium rod, and the thickness of the pre-laid filter layer is 2-4 mm.

[0039] The specific process of recirculating filtration and pre-spreading on the titanium rod of the titanium rod filter is: after the diatomite is wetted with purified water, the above-mentioned mixer is used to stir continuously to avoid the diatomite from settling to obtain a suspension of ...

Embodiment 2

[0042] Prepare sodium hyaluronate by the existing extraction method, take 41--58 grams of diatomite, add 4L of water to moisten it, and stir evenly to obtain a suspension of diatomite. In the state of stirring, use a pump to pre-spread the wetted diatomite circulation filter on the titanium rod, so that the thickness of the diatomite pre-spread on the titanium rod is 5-7 mm. The mixer and pump used are the same as in Example 1. The concrete process of circulation filtration is also with embodiment 1.

[0043]Then use purified water or water for injection to wash the pre-laminated filter layer circularly for 2-5 times. Take 1500 ml of enzymolysis solution, add 7.5 grams of activated carbon to absorb for 30 minutes, then pass the enzymolysis solution through the pre-laid filter layer with positive or negative pressure for decarbonization and filtration. After the decarburized and filtered medicinal solution is subjected to cetylpyridinium chloride CPC complex precipitation, it ...

Embodiment 3

[0045] Prepare sodium hyaluronate by the existing extraction method, take 66-82.5 grams of diatomite, add 4L of water to moisten it, and stir evenly to obtain a suspension of diatomite. In the stirring state, use a pump to pre-spread the wetted diatomite circulation filter on the titanium rod, so that the thickness of the diatomite pre-spread on the titanium rod is 8-10 mm. The mixer and pump used are the same as in Example 1. The concrete process of circulation filtration is also with embodiment 1.

[0046] Then use purified water or water for injection to wash the pre-laminated filter layer circularly for 2-5 times. Take 1500 ml of enzymolysis solution, add 7.5 grams of activated carbon to absorb for 30 minutes, then pass the enzymolysis solution through the pre-laid filter layer with positive or negative pressure for decarbonization and filtration. After the decarbonized and filtered medicinal solution is subjected to cetylpyridinium chloride CPC complex precipitation, it ...

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Abstract

The invention relates to hyaluronic acid, in particular to method for purifying hyaluronic acid by pre-laying filter aid. In the step of kieselguhr and active carbon absorption and filtration, after the kieselguhr is pre-laid on a titanium bar filter as a filter layer, the enzymolysis solution or fermentation solution is filtered. When the method is used for preparing hyaluronic acid, the filtration assisting and absorbing effects of the kieselguhr are increased, the light transmittance of the filtrate can be improved, the bacterial endotoxin content of medicinal liquid is lowered effectively, and the impurities introduced to the medicinal liquid with the use of the kieselguhr are avoided. Compared with the overseas plate type coarse filtering method, the method reduces production cost, achieves the same effect and is particularly suitable for industrial production.

Description

technical field [0001] The invention relates to hyaluronic acid, in particular to a method for purifying hyaluronic acid by pre-laying a filter aid. Background technique [0002] Hyaluronic acid (HA for short), also known as hyaluronic acid or hyaluronic acid, is a macromolecular mucopolysaccharide composed of (1→3)-2-acetylamino-2-deoxy-β-D-glucose- A linear polysaccharide composed of (1→4)-O-β-D-glucuronic acid disaccharide repeating units, the molecular formula is (Cl 4 h 21 NO 11 ) n , Widely present in the connective tissue of organisms, depending on the source of the tissue, the range of molecular weight is 2×10 5 ~7×10 6 , the number of disaccharide units is 300 to 11,000 pairs. Has the following general structural formula (1): [0003] [0004] Commercial hyaluronic acid is generally in the form of sodium salt, white fibrous or powdery solid, has strong hygroscopicity, is soluble in water, and insoluble in organic solvents. The macromolecular network struc...

Claims

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Application Information

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IPC IPC(8): C08B37/08B01D37/02
Inventor 马贤鹏李建勇陈亮
Owner SHANGHAI JINGFENG PHARMA
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