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Homologous system model method for quantitatively detecting bacterial endotoxin of blood

A quantitative detection method and technology of bacterial endotoxin, applied in the direction of measuring devices, biological tests, material inspection products, etc., can solve problems such as irrationality and differences in reaction trends

Inactive Publication Date: 2004-01-21
湛江安度斯生物有限公司
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Problems solved by technology

[0011] There is a serious defect in the existing endotoxin quantitative detection method: when preparing the standard curve, the standard endotoxin is diluted to the required concentration with water (W), and then reacted with TAL (hereinafter referred to as the reaction between endotoxin and TAL for T / E reaction)
[0020] 2. Differences in reaction trends
[0024] The above comparative analysis shows the serious defect of the existing quantitative detection method of bacterial endotoxin, that is to use the mathematical model established by one reaction system to measure the reaction of another different system! this is very unreasonable

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  • Homologous system model method for quantitatively detecting bacterial endotoxin of blood
  • Homologous system model method for quantitatively detecting bacterial endotoxin of blood
  • Homologous system model method for quantitatively detecting bacterial endotoxin of blood

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Embodiment Construction

[0050] 1. The steps to establish the same system model are as follows:

[0051] a) Determine the standard endotoxin concentration required to establish the standard curve. e.g.E 0.05 ,E 0.01 and E 0.002 .

[0052] b) Take a bottle (bottle) of standard endotoxin (RSE, ISE, NSE or CSE), reconstitute and vortex according to the instructions for use and prepare for use.

[0053] c) Determine the detection concentration of the sample. For example, the detection concentration of blood samples is 1:10 dilution (S 10 ).

[0054] d) Dilute the standard endotoxin to the required concentration using the sample of detection concentration. For example, the S of the dynamic time nephelometric method 10 E. 0.05 , S 10 E. 0.01 and S 10 E. 0.002 . It is also possible to use the mutual release of the sample (S) and the standard endotoxin to obtain a test concentration sample containing the desired concentration of endotoxin. In addition, a test concentration sample S without stan...

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Abstract

The present invention relates to homologous system model method for quantitatively detecting bacterial endotoxin of blood, and aims at solving the problem of difference or interference caused by different system property. In the system model method, one new concept is proposed for solving the BET interference of the sample, i. e., all the unkown interference factors are considered as systematic deviation. Once the systematic deviation is eliminated, the said BET interference of the sample will be eliminated. In some reaction system, digital model is established and sample is detected in the same system, the difference caused by different system property will be eliminated.

Description

technical field [0001] The invention relates to a quantitative detection method of bacterial endotoxin. Background technique [0002] Bacterial endotoxin is one of the components of the cell wall of Gram-negative bacteria and is a major pyrogen. Bacterial endotoxin detection has been more and more widely used in modern medicine and pharmaceutical industry. [0003] The reagent used in bacterial endotoxin detection is Limulus reagent (TAL or LAL). TAL contains factor C, factor B, coagulase, and coagulogen that can be activated by very small amounts of bacterial endotoxin. Under suitable conditions (temperature, pH value and no interfering substances, etc.), bacterial endotoxin can cause TAL to produce agglutination reaction. [0004] The methodological classification of bacterial endotoxin detection is as follows: [0005] I. Limit detection method (gel method) [0006] II. Quantitative detection method [0007] The principle of the quantitative detection method of ba...

Claims

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Application Information

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IPC IPC(8): G01N21/25G01N33/52G01N33/559G01N33/579
Inventor 冯聚锦
Owner 湛江安度斯生物有限公司
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