Preparation and application of detection kit for blood bacterial endotoxin

A bacterial endotoxin and blood technology, applied in the preparation of test samples, material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problem of lack of bacterial endotoxin and glucose in body fluids. The rapid separation and extraction of glycans, the inability to identify the specific reaction between bacterial endotoxin and Limulus reagent, and the poor resolution of bacterial endotoxin and dextran components have achieved the effects of improving optical specificity, ensuring reliability, and verifying accuracy

Active Publication Date: 2013-01-30
湛江博康海洋生物有限公司
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Problems solved by technology

At the same time, (1,3)-β-D-glucan can activate the G factor in the common limulus reagent to cause a bypass reaction, causing agglutination reaction and causing false positive results in bacterial endotoxin detection
Therefore, the specific reaction between bacterial endotoxin and LAL has been unable to be distinguished due to the

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  • Preparation and application of detection kit for blood bacterial endotoxin
  • Preparation and application of detection kit for blood bacterial endotoxin
  • Preparation and application of detection kit for blood bacterial endotoxin

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Embodiment Construction

[0043] The principle of the technical solution provided by the present invention is: using the C factor of the specific Limulus reagent provided by the present invention to be activated by a small amount of bacterial endotoxin in the body fluid sample to generate a biochemical reaction, the temperature of the optical instrument is constant (36-38°C) Observation, using the correlation between the greater the concentration of bacterial endotoxin and the shorter the reaction time, the logarithm of the bacterial endotoxin concentration (C) and the reaction time (T) were respectively used for regression analysis (LgT=a+bLgC), and the measured Bacterial endotoxin content in body fluid samples. The bacterial endotoxin and / or (1,3)-βD glucan substance reacted with the reagent provided by the invention is identified through the optical density curve and the reaction rate, and the qualitative and quantitative detection of the bacterial endotoxin is realized on this basis. see figure 1 ...

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Abstract

The invention provides a specific limulus reagent and a preparation method thereof. The preparation method comprises steps of blood collection, cell separation, cell lysis, cell wall separation, addition of an activator and a G factor inhibitor, so as to prepare a semi-finished product. The invention further provides a detection kit for blood bacterial endotoxin, wherein the kit contains the specific limulus reagent, and further comprises a humoral treatment agent I, a humoral treatment agent II, bacterial endotoxin controls and inspection water. The specific limulus reagent and the detection kit provided by the invention can qualitatively identify a gel reaction of the limulus reagent with the bacteria endotoxin and glucan type substances, thereby improving reliability of the detection results of the blood bacterial endotoxin, and realizing quantitative detection for the blood bacterial endotoxin. By using the specific limulus reagent and the detection kit, the detection of the blood bacterial endotoxin is characterized by high sensitivity, reliable detection result and high detection efficiency.

Description

technical field [0001] The invention relates to the technical field of bacterial endotoxin detection, in particular to a specific limulus reagent and a blood bacterial endotoxin detection kit for detecting body fluid bacterial endotoxin. Background technique [0002] Limulus reagent is a biological detection reagent made from the blood amebocyte lysate of marine organism Limulus. Due to its high biological activity, convenient use and safety, it is mainly used for injection medicine, biological products, radiopharmaceuticals, hemodialysis Bacterial endotoxin detection of liquid, dialysis water, blood maintenance solution and medical devices (infusion and injection devices) has played an active role in ensuring the quality of drugs and drug safety. [0003] Bacterial endotoxin is a complex of lipid A component and trace protein on the cell wall of Gram-negative bacteria. The bacterial endotoxin molecule is an amide lipid complex with a disaccharide structure, and its molecula...

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Application Information

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IPC IPC(8): G01N21/78G01N21/82G01N1/38
Inventor 莫水晶莫世文莫世君
Owner 湛江博康海洋生物有限公司
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