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Preparation method of fermented bean pulp rich in function peptide for feeding

A technology for fermenting soybean meal and functional peptides, applied in application, animal feed, animal feed, etc., can solve the problems of poisoning, protein digestibility and biological function of soybean meal are far less than that, affecting the application value of soybean meal, etc. Beneficial for absorption and utilization

Inactive Publication Date: 2009-06-17
AGRI BIO TECH INST GUANGDONG ACADEMY OF AGRI SCI +1
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AI Technical Summary

Problems solved by technology

[0003] Soybean meal is the most widely used plant-based feed protein raw material in my country and even in the world. However, with the advancement of science and technology and the deepening of research, it has been found that about 80% of soybean protein has a relative molecular mass of more than 100kDa, and most of the molecules are reversed. The parallel β-helix has a non-ordered structure, and the molecules are highly compressed and folded, so that the digestibility and biological functions of soybean meal protein are far inferior to animal protein feeds such as fishmeal. In addition, there are various anti-nutritional factors in soybean meal that are also toxic to animals. , seriously affecting the application value of soybean meal in animal production

Method used

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  • Preparation method of fermented bean pulp rich in function peptide for feeding
  • Preparation method of fermented bean pulp rich in function peptide for feeding
  • Preparation method of fermented bean pulp rich in function peptide for feeding

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Embodiment Construction

[0018] An embodiment of a method for preparing fermented soybean meal rich in feed functional peptides provided by the present invention is as follows:

[0019] The strains used in this example are 3 strains of Bacillus subtilis, 1 strain of Lactobacillus, and 1 strain of Saccharomyces crevisiae. The specific steps are:

[0020] A. the LB liquid culture medium that is made up of 5 gram peptones, 5 gram yeast extracts, 3 gram sodium chloride and 1 liter of water (pH7.0), pours in the aseptic Erlenmeyer flask, makes Bacillus subtilis seed liquid culture medium and Liquid expansion medium; consisting of 2 grams of peptone, 5 grams of NaCl, 2 grams of glucose, 3 grams of KH 2 PO 4 , 3 grams K 2 HPO 4 , 0.2 g MgCl 2 , 0.05 g CaSO 4 , 2 grams of starch and 1 liter of water (pH6.5) to form a lactic acid bacteria medium, pour it into a sterile triangular flask to make a lactic acid bacteria seed liquid medium and a liquid expansion medium; 100 grams of malt extract powder, 0.01 ...

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Abstract

The invention discloses a method for producing fermentation bean pulp rich in feeding use functional peptides. The present invention relates to a manufacturing technique taking the dehulled bean pulp as raw materials, using the microbial fermentation technology to remove various nutrilit-resistance in the bean pulp and simultaneously obtaining a great deal of polypeptide fermentation bean pulps, specifically to a microbial fermentation process using multi-strain mixed solid fermentation for preparing bean pulp rich in feeding use functional peptides. The manufacturing technique including mixing uniformly a fermentation nutrient fluid composed of three strains of expansively trained Bacillus subtilis, one strain of Saccharomyces crevisiae and one strain of Lactobacillus according to the proportion 1.8:1:1; inoculating 5% of the inoculated amount into the cottonseed pulp the water content of 50% ( sieving with a 40 mesh sieve), simultaneously adding 5% of molasses, and mixing uniformly (with initial pH value of 7.0); aerobic fermentation at temperature of 40 DEG C for 48 hours, and then performing dry sampling at 60 DEG C, and finally performing nutrition component analysis to the bean pulp fore and after fermentation. The completed product of the fermentation bean pulp can be stored in a cool dry place for 6-9 months after weighing and packaging, which can be the feeds of pig, chicken, tilapia and other animals.

Description

technical field [0001] The invention relates to a production process of using peeled soybean meal as a raw material, using microbial fermentation technology to effectively remove various anti-nutritional factors in the soybean meal, and obtaining a large amount of polypeptide fermented soybean meal, in particular to a multi-species mixed solid fermentation preparation rich in Microbial fermentation process of soybean meal with functional peptides for feed. Background technique [0002] With the rapid development of animal husbandry and aquaculture, the demand for protein feed is increasing. However, due to food safety issues, especially in recent years, countries have restricted the production of fish meal and prohibited the use of animal protein such as meat and bone meal produced from leftovers of livestock products. Feed, therefore, people are paying more and more attention to the development and utilization of various plant protein sources. Among them, soybean meal, as a...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14A23K1/165C12N1/20A23K10/12A23K10/18A23K10/30A23K20/147
Inventor 邝哲师叶明强徐志宏丘银清赵祥杰罗国庆潘木水陈薇
Owner AGRI BIO TECH INST GUANGDONG ACADEMY OF AGRI SCI
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