Preparation method of fermented bean pulp rich in function peptide for feeding
A technology for fermenting soybean meal and functional peptides, applied in application, animal feed, animal feed, etc., can solve the problems of poisoning, protein digestibility and biological function of soybean meal are far less than that, affecting the application value of soybean meal, etc. Beneficial for absorption and utilization
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[0018] An embodiment of a method for preparing fermented soybean meal rich in feed functional peptides provided by the present invention is as follows:
[0019] The strains used in this example are 3 strains of Bacillus subtilis, 1 strain of Lactobacillus, and 1 strain of Saccharomyces crevisiae. The specific steps are:
[0020] A. the LB liquid culture medium that is made up of 5 gram peptones, 5 gram yeast extracts, 3 gram sodium chloride and 1 liter of water (pH7.0), pours in the aseptic Erlenmeyer flask, makes Bacillus subtilis seed liquid culture medium and Liquid expansion medium; consisting of 2 grams of peptone, 5 grams of NaCl, 2 grams of glucose, 3 grams of KH 2 PO 4 , 3 grams K 2 HPO 4 , 0.2 g MgCl 2 , 0.05 g CaSO 4 , 2 grams of starch and 1 liter of water (pH6.5) to form a lactic acid bacteria medium, pour it into a sterile triangular flask to make a lactic acid bacteria seed liquid medium and a liquid expansion medium; 100 grams of malt extract powder, 0.01 ...
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