Protein feed preparation method for using microorganisms for fermenting hermetia illucens larvae

A technology for microbial fermentation and feed protein, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as reducing animal feed intake, affecting the palatability and digestibility of feed, and improving palatability, The effect of removing the smell of larvae and being easy to use

Inactive Publication Date: 2019-08-23
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Soybean meal is the most widely sourced protein feed resource in my country's animal husbandry. The reason for limiting its application is the anti-nutritional factors it contains. The limiting factors of black soldier fly protein are mainly its own odor and chitin, which will affect the feed itself. palatability and digestibility, thereby reducing animal feed intake, how to make black soldier fly larvae and soybean meal into protein feed with the advantages of high nutrition, low toxin, low anti-nutritional factors, and no peculiar smell is a problem. A method with research value, for this reason we propose a method of using microorganisms to ferment black soldier fly larvae to prepare feed protein to solve the above problems

Method used

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  • Protein feed preparation method for using microorganisms for fermenting hermetia illucens larvae
  • Protein feed preparation method for using microorganisms for fermenting hermetia illucens larvae
  • Protein feed preparation method for using microorganisms for fermenting hermetia illucens larvae

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Embodiment 1

[0024] The invention provides a technical scheme: a method for preparing feed protein by using microorganisms to ferment black soldier fly larvae, characterized in that it comprises the following steps:

[0025] (1) Add water to 1L with 5g sodium chloride, 10g glucose, 10g peptone and 5g yeast extract to 1L, with natural pH, LB liquid medium prepared after 120~125℃ damp heat sterilization for 25min, pour it into a sterile triangle In the bottle, make Bacillus subtilis seed liquid culture medium and liquid expansion medium; By the tryptone of 2g, the NaCl of 5g, the glucose of 2g, the KH of 3g 2 PO 4 , 3g of K 2 HPO 4 , 0.2g of CaCl 2 , 0.05g of MgSO 4 Mix with 2g of starch and add water to 1L, PH6.5, lactic acid bacteria culture medium prepared after autoclaving at 121°C for 20 minutes, pour it into a sterile Erlenmeyer flask to make lactic acid bacteria seed liquid culture medium and liquid Expansion culture medium; the wort culture medium made by mixing 100g of malt ext...

Embodiment 2

[0045] The invention provides a technical scheme: a method for preparing feed protein by using microorganisms to ferment black soldier fly larvae, characterized in that it comprises the following steps:

[0046] (1) Add water to 1L with 5g sodium chloride, 10g glucose, 10g peptone and 5g yeast extract to 1L, with natural pH, LB liquid medium prepared after 120~125℃ damp heat sterilization for 25min, pour it into a sterile triangle In the bottle, make Bacillus subtilis seed liquid culture medium and liquid expansion medium; By the tryptone of 2g, the NaCl of 5g, the glucose of 2g, the KH of 3g 2 PO 4 , 3g of K 2 HPO 4 , 0.2g of CaCl 2 , 0.05g of MgSO 4 Mix with 2g of starch and add water to 1L, PH6.5, lactic acid bacteria culture medium prepared after autoclaving at 121°C for 20 minutes, pour it into a sterile Erlenmeyer flask to make lactic acid bacteria seed liquid culture medium and liquid Expansion culture medium; the wort culture medium made by mixing 100g of malt ext...

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Abstract

The invention discloses a protein feed preparation method for using microorganisms for fermenting hermetia illucens larvae. The method comprises the following steps of a, uniformly pulping 6-instar live larvae of fresh hermetia illucens through a pulping machine; b, mixing the pulp of the 6-instar larvae of hermetia illucens with soybean meal to obtain a fermentation raw material, and adjusting the humidity until the water content reaches 40-50% after uniform mixing of the mixed fermentation raw material; c, inoculating strains into the mixed fermentation raw material in step b, adding a strain activating nutrient prepared by mixing a carbon source, a nitrogen source and an inorganic salt according to the mass ratio of 2:1:0.1, fermenting and culturing the strains for 48-72 hours at the temperature of 28-32 DEG C, and drying and smashing the strains to obtain protein feed fermented by hermetia illucens. The method further promotes the effective utilization of the soybean meal and hermetia illucens in the livestock aquaculture industry and serves as one of the ways to solving the shortage problem of protein feed resources in China at present.

Description

technical field [0001] The invention relates to the technical field of protein feed, in particular to a method for preparing feed protein by using microorganisms to ferment black soldier fly larvae. Background technique [0002] Feed raw materials, especially the worldwide problem of shortage of protein feed resources. The lack of feed protein sources has become an important factor restricting the development of my country's feed industry, animal husbandry and aquaculture. Therefore, only by developing new protein source products, changing the current situation that the feed industry is overly dependent on several protein feeds, further expanding the source of protein feeds, and improving the limiting factors in existing protein feeds, can the aquaculture industry be maintained stable, healthy and sustainable develop. Therefore, the high-quality compound protein feed will definitely become a major trend in the development of the feed industry in the future, and microbial m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/10C12N1/20C12N1/18C12R1/125C12R1/865
CPCA23K10/12A23K10/10C12N1/20C12N1/18
Inventor 叶明强邝哲师周鹏飞黄静潘木水丘银清
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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