Protein feed preparation method for using microorganisms for fermenting hermetia illucens larvae
A technology for microbial fermentation and feed protein, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as reducing animal feed intake, affecting the palatability and digestibility of feed, and improving palatability, The effect of removing the smell of larvae and being easy to use
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Embodiment 1
[0024] The invention provides a technical scheme: a method for preparing feed protein by using microorganisms to ferment black soldier fly larvae, characterized in that it comprises the following steps:
[0025] (1) Add water to 1L with 5g sodium chloride, 10g glucose, 10g peptone and 5g yeast extract to 1L, with natural pH, LB liquid medium prepared after 120~125℃ damp heat sterilization for 25min, pour it into a sterile triangle In the bottle, make Bacillus subtilis seed liquid culture medium and liquid expansion medium; By the tryptone of 2g, the NaCl of 5g, the glucose of 2g, the KH of 3g 2 PO 4 , 3g of K 2 HPO 4 , 0.2g of CaCl 2 , 0.05g of MgSO 4 Mix with 2g of starch and add water to 1L, PH6.5, lactic acid bacteria culture medium prepared after autoclaving at 121°C for 20 minutes, pour it into a sterile Erlenmeyer flask to make lactic acid bacteria seed liquid culture medium and liquid Expansion culture medium; the wort culture medium made by mixing 100g of malt ext...
Embodiment 2
[0045] The invention provides a technical scheme: a method for preparing feed protein by using microorganisms to ferment black soldier fly larvae, characterized in that it comprises the following steps:
[0046] (1) Add water to 1L with 5g sodium chloride, 10g glucose, 10g peptone and 5g yeast extract to 1L, with natural pH, LB liquid medium prepared after 120~125℃ damp heat sterilization for 25min, pour it into a sterile triangle In the bottle, make Bacillus subtilis seed liquid culture medium and liquid expansion medium; By the tryptone of 2g, the NaCl of 5g, the glucose of 2g, the KH of 3g 2 PO 4 , 3g of K 2 HPO 4 , 0.2g of CaCl 2 , 0.05g of MgSO 4 Mix with 2g of starch and add water to 1L, PH6.5, lactic acid bacteria culture medium prepared after autoclaving at 121°C for 20 minutes, pour it into a sterile Erlenmeyer flask to make lactic acid bacteria seed liquid culture medium and liquid Expansion culture medium; the wort culture medium made by mixing 100g of malt ext...
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