Method for preparing composite protein feed by mixed fermentation

A technology of mixed fermentation and compound protein, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of affecting the palatability of feed, reducing animal feed intake, etc., to reduce the molecular weight of fermented compound feed protein, remove the odor of silkworm pupae, improve the palatability effect

Inactive Publication Date: 2012-10-24
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Soybean meal and peanut cake are the most widely sourced feed resources in my country's animal husbandry. The reason that limits their application is the anti-nutritional factors and aflatoxin contained in them. The limiting factor of silkworm chrysalis protein is mainly the peculiar smell of silkworm chrysalis, which will affect the feed itself. palatability, thereby reducing animal feed intake

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  • Method for preparing composite protein feed by mixed fermentation
  • Method for preparing composite protein feed by mixed fermentation
  • Method for preparing composite protein feed by mixed fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The strains used in this example are 2 strains of Bacillus subtilis, 1 strain of Lactobacillus, and 1 strain of Saccharomyces crevisiae. The specific steps are:

[0030] A. the LB liquid culture medium that is made up of 5 gram peptones, 5 gram yeast extracts, 3 gram sodium chloride and 1 liter of water (pH7.0), pours in the aseptic Erlenmeyer flask, makes Bacillus subtilis seed liquid culture medium and Liquid expansion medium; composed of 2 g tryptone, 5 g NaCl, 2 g glucose, 3 g KH 2 PO 4 , 3 grams K 2 HPO 4 , 0.2 g MgCl 2 , 0.05 g CaSO 4, 2 grams of starch and 1 liter of water (pH6.5) to form a lactic acid bacteria medium, pour it into a sterile triangular flask to make a lactic acid bacteria seed liquid medium and a liquid expansion medium; 100 grams of malt extract powder, 0.1 gram of chlorine Mycin and 1 liter of water (pH6.0) composed of wort culture medium, poured into a sterile Erlenmeyer flask to make yeast seed liquid culture medium and liquid expansion...

Embodiment 2

[0056] The difference from Example 1 is:

[0057] c. Inoculate the seed liquid with an inoculum size of 5% of the total weight of the medium by weight in the liquid expansion LB medium (Bacillus subtilis) and wort medium (brewer's yeast), respectively, and the Bacillus subtilis at a temperature of 37°C , 170rpm, shaker for 20 hours. Saccharomyces cerevisiae was cultured at 30° C., 170 rpm, and a shaking table for 28 hours. Inoculate the lactic acid bacteria seed solution with a weight of 5% of the total weight of the medium in the liquid expanded lactic acid bacteria culture medium (lactic acid bacteria), and inoculate the lactic acid bacteria seed liquid at a temperature of 35° C. for 48 hours. The strain fermentation culture liquid of Bacillus subtilis, brewer's yeast and lactic acid bacteria is uniformly mixed in a ratio of 1:2:2, and used as a strain liquid for fermentation of soybean meal, peanut cake and silkworm chrysalis mixed raw materials.

[0058] d. Weigh the soy...

Embodiment 3

[0062] The difference from Example 1 is:

[0063] c. Inoculate the seed liquid with an inoculum size of 5% of the total weight of the medium by weight in the liquid expansion LB medium (Bacillus subtilis) and wort medium (brewer's yeast), respectively, and the Bacillus subtilis at a temperature of 37°C , 170rpm, shaker for 20 hours. Saccharomyces cerevisiae was cultured at 30° C., 170 rpm, and a shaking table for 28 hours. Inoculate the lactic acid bacteria seed solution with a weight of 5% of the total weight of the medium in the liquid expanded lactic acid bacteria culture medium (lactic acid bacteria), and inoculate the lactic acid bacteria seed liquid at a temperature of 35° C. for 48 hours. The strain fermentation culture liquid of Bacillus subtilis, brewer's yeast and lactic acid bacteria is uniformly mixed according to the ratio of 1:1:1, and used as the strain liquid for fermentation of soybean meal, peanut cake and silkworm chrysalis mixed raw material.

[0064] d. ...

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Abstract

The invention discloses a method for preparing a composite protein feed by mixed fermentation, which is characterized by comprising the following steps: a, mixing soybean meals, peanut cakes and silkworms, and then adding water to obtain mixed fermentation raw materials; and b, inoculating microbial strains into the mixed fermentation raw materials in step a, adding a carbon source, mixing uniformly, fermenting and culturing for 48 to 72 hours at a temperature between 32 and 38 DEG C, and then drying to obtain the composite protein feed. The method further promotes the effective utilization of the soybean meals, the peanut cakes and the silkworms in animal husbandry and aquaculture, and becomes one of the ways to solve the problem of shortage of protein feed resources in China currently.

Description

technical field [0001] The invention relates to a production method for preparing compound protein feed through fermentation, in particular to a method for preparing compound protein feed through mixed fermentation. Background technique [0002] With the transformation of people's living standards from "subsistence" to "nutrition", the demand for meat, poultry, eggs and milk continues to grow, which in turn drives the rapid development of the livestock and poultry breeding industry, and also produces feed materials, especially protein feed. A worldwide problem of resource scarcity. The lack of feed protein sources has become an important factor restricting the development of my country's feed industry, animal husbandry and aquaculture. Therefore, only by developing new protein source products, changing the current situation that the feed industry is overly dependent on several protein feeds, further expanding the source of protein feeds, and improving the limiting factors i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K10/12A23K20/147
Inventor 叶明强邝哲师罗国庆廖森泰肖更生徐志宏赵祥杰潘木水
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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