Enzymolysis-fermentation coupled aquatic protein active peptide preparation method

Abstract

The invention discloses an enzymolysis-fermentation coupled aquatic protein active peptide preparation method. The method mainly includes the following steps that fresh aquatic protein is minced; the minced aquatic protein is added to an enzymolysis tank, nutrients and water are added, and the mixture is mixed to be uniform to serve as a fermentation culture medium; the enzymolysis tank is inoculated with high-temperature-resistant facultative anaerobe Bacillus methylotrophicus HL-4 capable of generating protease, and meanwhile enzymolysis and fermentation are conducted; fermentation liquor is subjected to filtration, ultra-filtration and nano-filtration in sequence, then concentrated liquor is obtained, and the finished active peptide is obtained after freeze drying of the concentrated liquor. According to the technology, the enzymolysis process and the fermentation process are completed in the enzymolysis tank at the same time, and equipment and operation are simplified; acting conditions are mild, the biological activity of functional components in the prepared functional peptide and the aquatic protein is better preserved, and good application prospects are achieved.

Description

technical field [0001] The invention relates to an enzymolysis-fermentation coupled method for preparing active peptides of aquatic proteins, which belongs to the field of processing and utilization of aquatic products. Background technique [0002] The small peptides produced by protein enzymatic hydrolysis are not only easy to digest and absorb, but also have anti-hypertensive, anti-cholesterol, anti-thrombosis, improve lipid metabolism, enhance human physical fitness, help restore fatigue, promote calcium, phosphorus and other trace elements. Absorption, promote brain development, improve memory, enhance the vitality of macrophages and B cells, enhance immune function, protect epidermal cells, prevent melanin precipitation, eliminate free radicals in the body, etc. [0003] A lot of research has been done in China on the preparation of aquatic protein active peptides. At present, the preparation methods of aquatic protein active peptides mainly include autolytic enzymati...

AI Technical Summary

Problems solved by technology

The above high-temperature treatments will weaken the biological activity of the prepared functional peptides or the functional components contained in the aquatic protein itself to a certain extent; in addition, the high-temperature treatment will significantly enhance the Maillard reaction, making the color of the enzymatic hydrolyzate darker, which is suitable for the finished product processing The process increases the burden

Secondly, the method of enzymatic hydrolysis with external enzymes requires the addition of proteases, which increases the cost; autolytic enzymatic hydrolysis is not complete due to the lack of endogenous enzymes

In addition, although the microbial fermentation method does not use external proteases, the raw materials need to be sterilized to inactivate the endogenous enzymes, which cannot fully utilize the endogenous enzymes effectively.