Tumor enzyme activated quantum dot probe, preparation method and application thereof

A tumor enzyme and quantum dot technology, applied in the field of tumor enzyme-activated quantum dot probes and their preparation, can solve the problems of wide emission spectrum and narrow excitation spectrum, etc.

Inactive Publication Date: 2015-06-17
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional organic dyes have a narrow excitation spectrum and a broad emission spectrum

Method used

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  • Tumor enzyme activated quantum dot probe, preparation method and application thereof
  • Tumor enzyme activated quantum dot probe, preparation method and application thereof
  • Tumor enzyme activated quantum dot probe, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Synthesis and purification of embodiment 1 mercaptopropionic acid stable quantum dots

[0064] a Weigh 25.4mg of tellurium powder (purchased from Sinopharm Chemical Reagent Co., Ltd.) and 50mg of sodium borohydride (purchased from Sinopharm Chemical Reagent Co., Ltd.) into a round bottom flask, add 5ml of ultrapure water under nitrogen protection, 80 ℃ oil bath reaction 1-1.5h to purple, that is, sodium hydride telluride precursor solution.

[0065] b Weigh 45.7 mg of cadmium chloride hydrate (purchased from Sinopharm Chemical Reagent Co., Ltd.), add 30 μL of mercaptopropionic acid solution with a purity of 99% (purchased from Alfa Aesar), continue to add 40 ml of ultrapure water, stir until fully dissolved, and adjust pH to 11.9, under the protection of nitrogen, add dropwise one-tenth of the sodium telluride hydride precursor solution prepared in step a, and reflux the reaction at an appropriate time, monitor the reaction result with a fluorescence spectrophotometer (...

Embodiment 2

[0068] Synthesis of Example 2 Thiolated Low Molecular Weight Heparin

[0069] a Dissolve 50mg of low molecular weight heparin (enoxaparin sodium, molecular weight 3500-5500, purchased from Hebei Changshan Biochemical Pharmaceutical Co., Ltd.) in 10ml of citric acid buffer (0.1M, pH3.0), add 21.4mg of periiodine sodium heparin, react at room temperature for 2 hours, and dialyze with a dialysis bag with a molecular weight cut-off of 3500 to obtain oxidized low-molecular-weight heparin.

[0070] b. React the oxidized low-molecular-weight heparin prepared in step a with 11.4 mg of cysteine ​​hydrochloride in a total volume of 20 ml of phosphate buffer (0.1 M, pH 7.0) for 2 h at room temperature.

[0071] c. Slowly add 10ml of newly prepared 0.33M sodium borohydride aqueous solution dropwise to b, react at room temperature for 1 hour, dialyze with a dialysis bag with a molecular weight cut-off of 3500, and store at 4°C for later use.

Embodiment 3

[0072] Example 3 Synthesis of thiolated low molecular weight heparin-modified quantum dots

[0073] a. Add 1 / 5 volume of newly prepared 0.33M sodium borohydride aqueous solution to the stock solution obtained in Example 2, and react at room temperature for 1 h.

[0074] B the mercaptopropionic acid stable quantum dot obtained in embodiment 1 is diluted to OD with PBS 310 =1.0, add different amounts obtained from step a in Example 3, and react in the dark for 12 hours.

[0075] c Purification: Add 3-4 times the volume of isopropanol to b, mix well, centrifuge at 8000g for 20min, discard the supernatant, add 10-20ml of isopropanol, mix well, centrifuge at 8000g for 20min, discard the supernatant, and vacuum dry at 60°C 2h that is.

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Abstract

The invention relates to a tumor enzyme activated quantum dot probe, which is modified and contains a functional peptide; wherein one end of the functional peptide is connected to a quenching agent, and the tumor enzyme can specifically recognize and cut off the functional peptide to light the quantum dots. The tumor enzyme activated quantum dot probe is in a fluorescence quenched state in the normal tissue and gives off fluorescence in the tumor part; namely the quantum dot has dual functions: invisibility in the normal tissue and tumor enzyme sensitivity in the tumor part, and thus the probe can diagnose tumor. The invention further provides a preparation method of the quantum dot probe, and an application of the quantum dot probe in preparation of reagents for tumor diagnosis. The provided quantum dot probe can diagnose tumor and is advantageously used as a precursor probe for tumor diagnosis.

Description

technical field [0001] The present invention relates to a precursor probe for tumor diagnosis and detection as well as its preparation method and application, and more specifically relates to a tumor enzyme-activated quantum dot probe as well as its preparation method and application. The tumor enzyme-activated quantum dot probe of the invention is a probe that can effectively diagnose and detect tumors. Background technique [0002] As one of the largest public health problems in the world, tumors seriously endanger human health. According to reports, in my country, the incidence of cancer is increasing year by year, and the mortality rate is as high as 87%. It has become the first cause of death of Chinese urban and rural residents. In contrast, patients with early-stage cancers are much healthier with treatment than those with advanced-stage cancers. If early diagnosis and detection of tumors can be performed and targeted treatment can be performed, the survival rate of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00C09K11/88C09K11/02C07K7/08C07K14/00
Inventor 黄永焯王雅萍
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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