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Modified chromatographic apparatus and method of using same in continuous-flow chromatography

A chromatography device and flow chromatography technology, applied in separation methods, chemical instruments and methods, biochemical cleaning devices, etc., can solve the problems of inability to identify, stay in the design stage, and AKTApure cannot be implemented.

Active Publication Date: 2017-12-19
SUZHOU YAOMING KANGDE INSPECTION TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitations of AKTAexplorer's own software, it can only support up to four seven-way valves at the same time. However, two of the seven-way valves exist as extension valves, which cannot be effectively recognized by the software and cannot be operated correctly in the software.
Therefore, its design can only stay in the design stage and cannot be put into practical use.
In addition, AKTAexplorer is a device that has been out of production for many years, and there are no new products for sale. In practice, it is found that even if the existing AKTAexplorer device is modified according to the patented method, it is completely incapable of current research and development due to the limitations of device performance and functions. And the high-intensity requirements of continuous flow chromatography for several months of continuous operation in production
There are significant differences in system design and function between AKTAexplorer and AKTApure of the present invention, and the modification scheme for AKTAexplorer cannot be implemented in AKTApure of the present invention

Method used

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  • Modified chromatographic apparatus and method of using same in continuous-flow chromatography
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  • Modified chromatographic apparatus and method of using same in continuous-flow chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] An improved AKTApure chromatography device, including standard AKTApure (pure M) configuration: system pump (A pump, B pump), mixing tank, sample pump, system pump A pump inlet valve (Inlet A), injection valve (Injection valve) Valve), first column valve, first UV detector, conductivity detector, pH detector, backpressure valve, and outlet valve; in addition, includes:

[0055] Three multi-function valves or mixer valves, which are defined in the system as Versatile Valve 1 (V1), Versatile Valve 2 (V2) and Versatile Valve 3 (V3); a second column valve or a Loop ring Valve, and define it as Loop Valve (L) in the system; and three columns No. 1 column, No. 2 column and No. 3 column;

[0056]Among them, the Out position of the first column valve is connected to the LoopF ​​position of the injection valve, 1A and 3B of the first column valve are connected to the No. 1 position of V1 with a tee, and 2A and 1B of the first column valve are connected with a tee Connect to No....

Embodiment 2

[0060] An improved AKTApure chromatography device, including standard AKTApure (pure M) configuration: system pump (A pump, B pump), mixing tank, sample pump, system pump A pump inlet valve (Inlet A), injection valve (Injection valve) Valve), first column valve, UV detector, conductivity detector, pH detector, back pressure valve and outlet valve; also includes:

[0061] Three multi-function valves or mixer valves, which are defined in the system as Versatile Valve 1 (V1), Versatile Valve 2 (V2) and Versatile Valve 3 (V3); a second column valve or Loop ring valve , and define it as Loop Valve (L) in the system; a second UV detector, and define it as UV 2nd in the system; and three columns No. 1 column, No. 2 column and No. 3 column;

[0062] Among them, the Out position of the first column valve is connected to the LoopF ​​position of the injection valve, 1A and 3B of the first column valve are connected to the No. 1 position of V1 with a tee, and 2A and 1B of the first column...

Embodiment 3

[0066] An improved AKTApure chromatography device, including standard AKTApure (pure M) configuration: system pump (A pump, B pump), mixing tank, sample pump, system pump A pump inlet valve (Inlet A), injection valve (Injection valve) Valve), Column Valve, UV Detector, Conductometric Detector, pH Detector, Backpressure Valve, and Outlet Valve; in addition, includes:

[0067] Three multi-function valves or mixer valves, which are defined in the system as Versatile Valve 1 (V1), Versatile Valve 2 (V2) and Versatile Valve 3 (V3); a second column valve or Loop ring valve , and define it as Loop Valve (L) in the system; a second UV detector, and define it as UV 2nd in the system; and three columns No. 1 column, No. 2 column and No. 3 column;

[0068] Among them, the Out position of the first column valve is connected to the LoopF ​​position of the injection valve, 1A and 3B of the first column valve are connected to the No. 1 position of V1 with a tee, and 2A and 1B of the first co...

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Abstract

The invention discloses a modified AKTA pure chromatographic apparatus. On the basis of the structure of original standard AKTA pure, three multifunctional valves, as well as a second column position valve or a Loop ring valve, are added, so that two flow paths, which work simultaneously, are formed by three columns; one flow path is used for continuously loading a sample, while the other one flow path is used for pretreatment before and post-treatment after sample loading, so that continuous purification of a perfusion culture material solution is achieved. The modified apparatus can achieve low-cost, multi-cycle and automated purification of fed-batch culture solutions, avoids accumulation and degradation of the sample, can maintain the stability of the sample, is free of an extra large-volume buffer container, and is high in purification efficiency.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an improved chromatographic device used for continuous purification of perfusion culture feed liquid and low-cost, multi-cycle and automatic purification of fed-batch culture feed liquid. Background technique [0002] AKTApure chromatography equipment was originally used for ordinary single-column chromatography, such as figure 1 As shown, it is a schematic diagram of the pipeline connection of the standard AKTApure chromatography equipment, including the system pump (A pump, B pump), the mixing tank (Mixer), the sample pump (Sample Pump), the system pump A pump inlet valve (Inlet A), Injection Valve, Column Valve, UV Detector (UV), Conductivity Detector (Conductivity), pH Detector, Back Pressure Valve (pH&Restrictor) and Outlet Valve (Outlet), among which, injection The Col position of the valve is connected to the In position of the column valve, and the Out position of the column...

Claims

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Application Information

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IPC IPC(8): B01D15/10B01D15/18C12M1/00
CPCB01D15/10B01D15/1864C12M99/00
Inventor 唐思远沈克强周伟昌陈智胜
Owner SUZHOU YAOMING KANGDE INSPECTION TESTING
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