Issatchenkia orientalis strain, preparation method thereof and method for preparing xylitol and ethanol by using same

A technology of Isaac yeast and xylitol, applied in the field of detoxification active microbial strains, can solve the problems of cumbersome process and high cost

Inactive Publication Date: 2010-08-18
唐传生物科技(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to solve the problems of cumbersome process and high cost in the existing hemicellulose hydrolysis physical chemical detoxification process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0079] operation 1

[0080] Weigh 3 kg of bagasse that has been washed and re-dried, add H2SO4 solution (2.5%, w / w) at a solid-to-liquid ratio (w / v) of 1:7, hydrolyze at 120 ° C for 2 h, collect the filtrate by centrifugation, wash the filter residue once, and combine The filtrate was adjusted to pH 3 with solid calcium carbonate, and the precipitate was removed by suction filtration to obtain bagasse hemicellulose hydrolyzate. The reducing sugar content of the hydrolyzate is 3%, and xylose monosaccharide accounts for more than 80% of the total sugar.

[0081] operation 2

[0082] Take 2 kg of crushed dry corncobs, add 2% (w / w) H at a solid-to-liquid ratio of 1:7 2 SO 4 The solution was hydrolyzed at 120°C for 2 hours, the liquid part was collected, neutralized with calcium carbonate to a pH value of 3, and the precipitate was removed by suction filtration to obtain a dilute acid hydrolyzate of corncob hemicellulose. The total sugar content of the hydrolyzate is 5%, wherei...

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Abstract

The invention relates to a microbial strain of Issatchenkia orientalis (S-7) CCTCC NO:M 206098. The homology between nucleotide characteristic sequence of the 26S rDNA D1/D2 area of the strain and a characteristic sequence with register number of EF030708 in the GenBank is 100%. The invention further discloses a method for preparing the strain and the bio-detoxification application of the strain. The strain is mixed and inoculated to the microorganism fermenting xylose to produce xylitol, thereby realizing the production of the xylitol by synchronously detoxifying and fermenting hemicellulose hydrolysate; and a detoxified active strain is mixed and inoculated to the microorganism fermenting xylose, thereby realizing the production of the ethanol synchronously detoxifying and fermenting the hemicellulose hydrolysate. Compared with the traditional technology, the invention has higher production efficiency and higher quality of products.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for biological detoxification of hemifiber hydrolyzate and special detoxification active microbial strains. Background technique [0002] Hemicellulose is a polysaccharide other than cellulose in the plant cell wall. The content varies with different plants, accounting for 20-35% of the weight of the plant. It is the most abundant polysaccharide on the earth except cellulose. [0003] Cellulose is a homogeneous polysaccharide composed entirely of glucose units, while hemicellulose is mainly composed of xylose with β-(1-4) glycosidic bonds to form the main chain, and arabinose, mannose and galactose form branches. Non-uniform glycans with a chain structure. The xylan backbone of hemicellulose generally contains 50-150 xylose units, has no crystalline structure, and is bound to the surface of cellulose microfibrils. [0004] Hemicellulose is much easier to hydrolyze th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P19/02C12P7/10C12R1/74C12R1/645
CPCY02E50/16Y02E50/10
Inventor 张厚瑞覃香香蔡爱华周玉恒陈海珊
Owner 唐传生物科技(厦门)有限公司
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