Separation of dye degradation strain and application thereof

An azo dye and strain technology, applied in the field of environmental microorganisms, can solve the problems of high level of organic pollutants and complex waste water sources.

Inactive Publication Date: 2011-08-31
GRADUATE SCHOOL OF THE CHINESE ACAD OF SCI GSCAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The sources of wastewater near printing and dyeing factories are complicated, and the level of organic pollutants is high
At present, there is no literature report on the degradation of dyes by Issakula orientalis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The cultivation of embodiment 1 thalline

[0015] Use an inoculation loop to pick the yeast strain Issatchenkia orientalis YP-1 and connect it to the slant for the activation of the above-mentioned strains, and culture it in an incubator with a temperature set at 28°C for 1-3 days. Reflective with etched edges. Then, use an inoculation loop to pick the well-grown thalline from the inclined surface and connect it to a conical flask equipped with nutrient solution, and set it at 28° C. at a temperature, and cultivate it on a shaker at 200 rpm for 1-3 days, and the gained thalline is centrifuged ( 9000rmp) for 10min, the thallus was washed with sterile physiological saline (0.8%) and sterile water, and then centrifuged, repeated 2-3 times, to obtain the white and pollution-free yeast cells.

[0016] As mentioned above, the culture medium for cultivating the strain consists of 20 g of peptone, 10 g of yeast powder, 20 g of sodium chloride, 20 g of agar plus water to 1 L, p...

Embodiment 2

[0018] Embodiment 2: the decolorization effect of azo dye degrading bacteria agent to azo dye in shake flask

[0019] 1) Inoculate 1 ml of bacterial solution in 100 ml of decolorized medium; use medium without dye as a control group.

[0020] 2) Samples were taken every 3 hours, and the absorbance of the culture solution at the maximum absorption peak of the dye was measured by an ultraviolet spectrophotometer. Decolorization rate (%)=(A-B) / A×100, wherein: A is the OD value of the decolorized culture medium before decolorization, and B is the OD value of the decolorized culture medium after decolorization. Under the culture conditions, the decolorization rate of the yeast strain Issatchenkia orientalis YP-1 can reach more than 99% within 12 hours.

[0021] The dye-degrading bacterial strain provided by the present invention was preserved in the General Microorganism Center of the China Microbiological Culture Collection Management Committee, and the preservation date was Dece...

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PUM

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Abstract

The invention relates to a yeast strain, which is separated from soil and can degrade azo dye with high efficiency, and a method utilizing the strain for performing decolorization on the dye, belonging to the field of biotechnologies. The strain is identified as Issatchenkia orientalis YP-1 and collected in China General Microbiological Culture Collection Center with the collection number being CGMCC No. 4523. By comparing the sequence of the strain with BLAST (basic local alignment search tool) retrieval in a Genbank, the sequence of the strain is found to be in high homology (reaching 99%) with Issatchenkia orientalis strain QD15.126SrRNA (ribonucleic acid) gene sequence. The pure strain is obtained by large-quantity screening from the soil. The strain can grow well in a culture medium taking the dye as a carbon source and culture solution, and has good decolorization effect against various azo dyes. A degradation bacterial agent produced according to the technical scheme of the invention can degrade the azo dye in the environment and protect the ecological environment.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and relates to a yeast strain (Issatchenkia orientalis YP-1) isolated from soil and having a good degradation effect on azo dyes, and an application thereof. Background technique [0002] Azo dyes are the most numerous varieties of synthetic dyes, widely used in textile, dyeing and printing industries, accounting for about 80% of the total organic dye products. Their molecular structures contain azo groups (-N=N-), and these groups are often connected with one or more aromatic ring systems to form a conjugated system as a chromophoric group for dyes. The high chroma of the dye will affect the normal function of the water body, lead to a decrease in the light transmittance of the water body, inhibit the natural process of photosynthesis in the water body, and then affect the growth of consumers at all levels and destroy the water body ecosystem. With the rapid development of dyes and p...

Claims

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Application Information

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IPC IPC(8): C12N1/16C02F3/34C12R1/645
Inventor 余志晟于影张慕明金德才张洪勋
Owner GRADUATE SCHOOL OF THE CHINESE ACAD OF SCI GSCAS
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