Complex microbial inoculant for fermenting and regenerating chicken manure and application of complex microbial inoculant

Abstract

The invention relates to a complex microbial inoculant for fermenting and regenerating chicken manure and application of the complex microbial inoculant. The complex microbial inoculant consists of a first complex microbial inoculant and a second complex microbial inoculant which are independently packaged, bacterial strains in the first complex microbial inoculants consist of bacillus coagulans, bacillus subtilis, aspergillus niger and saccharomyces cerevisiae; the bacterial strain in the second complex microbial inoculant is composed of lactobacillus plantarum, lactobacillus acidophilus and lactobacillus buchneri. According to the invention, seven probiotics are combined for use, the first complex microbial inoculant performs aerobic fermentation on a substrate containing chicken manure, unstable factors in chicken manure raw materials are reduced, and a high-quality raw material basis is provided for regenerated feed; wherein the second complex microbial inoculant performs anaerobic fermentation on a substrate containing chicken manure, so that the growth of harmful bacteria is inhibited, the safety of the regenerated product is further ensured, beneficial metabolites are generated, and the palatability of the regenerated product is improved. The finally obtained regenerated feed not only can reduce the feeding cost, but also can regulate the intestinal health, promote the growth of animals and reduce the morbidity.

Description

Technical Field

[0001] The present invention belongs to the technical field of recycled feed, and relates to a composite microbial agent for chicken manure fermentation and regeneration and its application. Background Art

[0002] With the large-scale and high-speed development of the breeding industry in China, the inventory and slaughter volume of poultry across the country have continued to increase. Among them, broilers and laying hens are the main forces in the market demand. Such a large-scale breeding volume has gradually highlighted the disadvantages of large fluctuations in the breeding industry market, rising feed costs, and increasing environmental protection pressure.

[0003] Because the intestinal tract of chickens is relatively short and the feed absorption rate is only about 30%, the feces excreted by them are rich in nutrients such as protein, minerals, and vitamins. Through fermentation treatment, it can be converted into high-quality feed, which helps to enrich the feed source and improve the cost performance of the feed. Secondly, fermenting chicken manure can reduce its environmental pollution, reduce waste emissions, and realize the recycling of resources. Therefore, chicken manure fermented recycled feed has great potential in resource utilization and environmental improvement.

[0004] However, chicken manure recycled feed currently also faces many challenges. Technically, it is difficult to control the fermentation process, such as the regulation of temperature, humidity, and microbial community. If not controlled properly, it may affect the quality and safety of the feed. In terms of quality, harmful substances such as pathogens, heavy metals, and antibiotics present in chicken manure, if not treated thoroughly, will pose a threat to animal health and thus affect food safety. This is also the main concern of consumers about chicken manure fermented feed, fearing that it will have an adverse impact on the quality of animal products and thus hinder market acceptance. In addition, the preparation of recycled feed from chicken manure usually undergoes harmless treatment through high-energy-consuming equipment such as high temperature and high pressure, resulting in disadvantages such as complex production processes, high energy consumption, low output ratio, incomplete odor treatment, and poor palatability.

[0005] Therefore, developing a strategy to efficiently convert chicken manure into harmless recycled feed can effectively reduce production costs and increase benefits. This not only reduces the dependence of the breeding industry on traditional feed raw materials but also provides a new and sustainable feed source, which conforms to the concept of sustainable development. It helps to guide the breeding industry to transform towards a more environmentally friendly and green direction, promote the sustainable development of the entire industry, and contribute to building an ecological friendly society. Summary of the Invention

[0006] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a composite microbial agent for chicken manure fermentation and regeneration and its application.

[0007] To achieve the purpose of this invention, the following technical solutions are adopted by the present invention:

[0008] In a first aspect, the present invention provides a composite bacterial agent for the fermentation and regeneration of chicken manure, and the composite bacterial agent for the fermentation and regeneration of chicken manure is composed of a first composite bacterial agent and a second composite bacterial agent which are independently packaged;

[0009] The strains in the first composite bacterial agent are composed of Bacillus coagulans, Bacillus subtilis, Aspergillus niger, and Saccharomyces cerevisiae; the strains in the second composite bacterial agent are composed of Lactobacillus plantarum, Lactobacillus acidophilus, and Lactobacillus buchneri.

[0010] The present invention has developed a brand-new bacterial agent capable of fermenting and regenerating chicken manure, which combines the above seven probiotics and sequentially performs aerobic fermentation and anaerobic fermentation on the substrate containing chicken manure.

[0011] Among them, Bacillus coagulans, Bacillus subtilis, Aspergillus niger, and Saccharomyces cerevisiae simultaneously perform aerobic fermentation on the substrate containing chicken manure. This process can effectively kill pathogenic bacteria and eggs in chicken manure, degrade toxic and harmful substances such as antibiotics, and minimize unstable factors (such as odors and toxins) in the chicken manure raw material, providing a high-quality raw material basis for the regenerated feed. Moreover, these four bacteria have a synergistic effect in exerting the above-mentioned effects.

[0012] Among them, Lactobacillus plantarum, Lactobacillus acidophilus, and Lactobacillus buchneri simultaneously perform anaerobic fermentation on the substrate containing chicken manure. This process can inhibit the growth of harmful bacteria, generate beneficial metabolites, improve the palatability of the regenerated product, and further ensure the safety of the regenerated product. Moreover, these three bacteria have a synergistic effect in exerting the above-mentioned effects.

[0013] The finally obtained regenerated product has stable nutrients, retains nutrients to the greatest extent, and the contents of molds and bacteria meet the feed standards. It can be used as feed or feed additive for feeding animals, which can not only reduce the feeding cost, but also does not affect the growth performance of animals. Moreover, the probiotics in the regenerated feed can be efficiently colonized in the animal intestine, regulate intestinal health through the synergistic effect between strains, promote animal growth, and reduce the incidence of diseases.

[0014] Preferably, the viable count ratio of Bacillus coagulans, Bacillus subtilis, Aspergillus niger, and Saccharomyces cerevisiae in the first composite bacterial agent is (0.5 - 2):(1 - 6):(1 - 5):(0.5 - 4).

[0015] Based on the potential synergistic cooperation relationship between Bacillus coagulans, Bacillus subtilis, Aspergillus niger, and Saccharomyces cerevisiae, when they meet the above specific viable count ratio, the effect is better.

[0016] Among them, the specific point values in (0.5 - 2) can be selected as 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2, etc.; among them, the specific point values in (1 - 6) can be selected as 1, 2, 3, 4, 5, 6, etc.; among them, the specific point values in (1 - 5) can be selected as 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, etc.; among them, the specific point values in (0.5 - 4) can be selected as 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, etc.; other specific point values within the above numerical ranges can be selected, and will not be elaborated one by one here.

[0017] Preferably, the viable count ratio of Lactobacillus plantarum, Lactobacillus acidophilus, and Lactobacillus buchneri in the second compound bacterium agent is (0.1 - 2):(0.2 - 3):(0.2 - 3).

[0018] Based on the potential synergistic cooperation relationship among Lactobacillus plantarum, Lactobacillus acidophilus, and Lactobacillus buchneri, when the above specific viable count ratio is satisfied, the effect is better.

[0019] Among them, the specific point values in (0.1 - 2) can be selected as 0.1, 0.3, 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2, etc.; among them, the specific point values in (0.2 - 3) can be selected as 0.2, 0.3, 0.5, 0.8, 1, 1.5, 2, 2.5, 3, etc.; other specific point values within the above numerical ranges can be selected, and will not be elaborated one by one here.

[0020] Preferably, the viable count in the first compound bacterium agent is not less than 2×10 10 CFU / g or 2×10 10 CFU / mL, such as 2×10 10 CFU / mL (CFU / g), 5×10 10 CFU / mL (CFU / g), 8×10 10 CFU / mL (CFU / g), 1×10 11 CFU / mL (CFU / g), 5×10 11 CFU / mL (CFU / g), etc.; other specific point values within this numerical range can be selected, and will not be elaborated one by one here.

[0021] Preferably, the viable count in the second compound bacterium agent is not less than 1×10 11 CFU / g or 1×10 11 CFU / mL, such as 1×10 11 CFU / mL (CFU / g), 1×10 12 CFU / mL (CFU / g), 5×10 12 CFU / mL (CFU / g), 1×10 13CFU / mL (CFU / g), etc.; other specific point values within this numerical range can be selected, and will not be elaborated one by one here.

[0022] Furthermore, the present invention also creatively discovers that when the Bacillus coagulans, Lactobacillus plantarum, Lactobacillus acidophilus, and Lentilactobacillus buchneri are the following self-isolated and preserved strains, the regenerated feed prepared therefrom has better effects in terms of safety, animal palatability, improvement of animal intestines, and promotion of animal growth performance.

[0023] Preferably, the Bacillus coagulans is the Bacillus coagulans BC66 strain with the preservation number CGMCC 21879.

[0024] Preferably, the Lactobacillus plantarum is the Lactobacillus plantarum LP194 strain with the preservation number CGMCC 21766.

[0025] Preferably, the Lactobacillus acidophilus is the Lactobacillus acidophilus LA71 strain with the preservation number CGMCC 21765.

[0026] Preferably, the Lentilactobacillus buchneri is the Lentilactobacillus buchneri LBu101 strain with the preservation number GDMCC No: 63094.

[0027] Preferably, the first compound bacterium agent and the second compound bacterium agent further include a carrier.

[0028] Preferably, the carrier includes zeolite powder.

[0029] Preferably, the first compound bacterium agent and the second compound bacterium agent further include a cell protector;

[0030] Preferably, the cell protector includes any one or a combination of at least two of skim milk, gelatin, dextrin, gum arabic, dextran, sodium alginate, polyvinylpyrrolidone, sucrose, lactose, trehalose, sorbitol, or xylitol.

[0031] Preferably, the first compound bacterium agent and the second compound bacterium agent further include a nutritional component.

[0032] Preferably, the dosage forms of the first compound bacterium agent and the second compound bacterium agent are independently a liquid agent or a powder.

[0033] The dosage form of the compound bacterium agent involved in the present invention is not limited, including the most commonly used solution agent, powder, or further prepared capsule agent, tablet agent, or granule agent.

[0034] In a second aspect, the present invention provides the use of the compound microbial agent according to the first aspect in the preparation of fermented and recycled chicken manure feed.

[0035] In a third aspect, the present invention provides a method for preparing fermented and recycled chicken manure feed, which is obtained by fermenting chicken manure with the compound microbial agent according to the first aspect, and includes the following steps:

[0036] (1) Inoculate the first compound microbial agent into the first material for aerobic fermentation to obtain a primary fermentation product;

[0037] (2) Inoculate the second compound microbial agent into the second material for anaerobic fermentation to obtain the fermented and recycled chicken manure feed;

[0038] The first material contains chicken manure; the second material contains the primary fermentation product.

[0039] Preferably, the inoculation amount of the first compound microbial agent is 0.02%-2%, such as 0.02%, 0.05%, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, 1.2%, 1.5%, 2%, etc.

[0040] Preferably, the inoculation amount of the second compound microbial agent is 0.001%-1%, such as 0.001%, 0.005%, 0.008%, 0.01%, 0.04%, 0.06%, 0.08%, 0.1%, 0.3%, 0.5%, 0.6%, 0.8%, 1%, etc.

[0041] Preferably, the first material further contains auxiliary materials, and the auxiliary materials include plant fibers, such as rice husk powder, corn straw powder, alfalfa grass, etc.

[0042] Preferably, the mass ratio of the first material to the auxiliary materials is (6-7):(3-4), such as 6:4, 6.2:3.8, 6.4:3.6, 6.6:3.4, 6.8:3.2, 7:3, etc.

[0043] Preferably, the second material further contains auxiliary materials, and the auxiliary materials include plant fibers, such as rice husk powder, corn straw powder, alfalfa grass, etc.

[0044] Preferably, the mass ratio of the second material to the auxiliary materials is (4-8):(2-6), such as 4:6, 5:5, 6:4, 7:3, 8:2, etc.

[0045] Preferably, the second material further contains an additionally added carbon source, such as glucose, molasses, etc.

[0046] Preferably, the carbon source is 2-10% of the mass of the second material, such as 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0047] Preferably, the moisture content of the first material is 55-60%, such as 55%, 56%, 57%, 58%, 59%, 60%, etc.

[0048] Preferably, the moisture content of the second material is 50-60%, such as 55%, 56%, 57%, 58%, 59%, 60%, etc.

[0049] Preferably, the aerobic fermentation is carried out at 55-70 °C (such as 55 °C, 58 °C, 60 °C, 62 °C, 64 °C, 66 °C, 68 °C, 70 °C, etc.) for 7-10 days (such as 7 days, 8 days, 9 days, 10 days, etc.).

[0050] Preferably, the anaerobic fermentation is carried out at 30-45 °C (such as 30 °C, 32 °C, 35 °C, 38 °C, 40 °C, 42 °C, 44 °C, 45 °C, etc.) for 7-10 days (such as 7 days, 8 days, 9 days, 10 days, etc.).

[0051] Other specific point values within the above numerical ranges are all within the protection scope of the present invention. For the sake of brief description, all point values will not be elaborated one by one here.

[0052] Fourthly, the present invention also provides the application of the chicken manure fermented and recycled feed prepared by the preparation method described in the third aspect in livestock breeding.

[0053] Compared with the prior art, the present invention has the following beneficial effects:

[0054] The present invention develops a brand-new bacterium agent capable of fermenting and recycling chicken manure. Seven probiotics are used in combination. Among them, the first compound bacterium agent can carry out aerobic fermentation on the substrate containing chicken manure, minimizing the unstable factors (such as odors and toxins) in the chicken manure raw material, providing a high-quality raw material basis for the recycled feed, and the four bacteria in the first compound bacterium agent have a synergistic effect in exerting the above-mentioned effects. Among them, the second compound bacterium agent can carry out anaerobic fermentation on the substrate containing chicken manure, inhibiting the growth of harmful bacteria, further ensuring the safety of the recycled product, and generating beneficial metabolites and improving the palatability of the recycled product. The three bacteria in the second compound bacterium agent have a synergistic effect in exerting the above-mentioned effects. The finally obtained recycled product can be used as animal feed or feed additive, which can not only reduce the feeding cost, but also does not affect the growth performance of animals, and can regulate intestinal health, promote animal growth, and reduce the incidence of diseases. Detailed Embodiments

[0055] To further elaborate on the technical means and effects adopted by the present invention, the following further illustrates the technical solution of the present invention in conjunction with the preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0056] The taxonomic name of the BC66 strain involved in the present invention is Bacillus coagulans. The preservation unit is the China General Microbiological Culture Collection Center. The preservation time is March 8, 2021. The preservation number is CGMCC 21879. The address is: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0057] The taxonomic name of the LP194 strain involved in the present invention is Lactobacillus plantarum. The preservation unit is the China General Microbiological Culture Collection Center. The preservation time is January 29, 2021. The preservation number is CGMCC 21766. The address is: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0058] The taxonomic name of the LA71 strain involved in the present invention is Lactobacillus acidophilus. The preservation unit is the China General Microbiological Culture Collection Center. The preservation time is January 29, 2021. The preservation number is CGMCC 21765. The address is: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0059] The taxonomic name of the LBu101 strain involved in the present invention is Lentilactobacillus buchneri. The preservation unit is the Guangdong Provincial Culture Collection Center of Microorganisms. The preservation time is December 26, 2022. The preservation number is GDMCC No: 63094. The address is: 5th Floor, Building 59, No. 100 Compound, Xianlie Middle Road, Guangzhou.

[0060] The preparation method of the bacterial powder involved in the following tests is as follows: Inoculate the required strain into MRS liquid medium, culture at 37°C for 24 h for activation, and activate continuously for 2 times to obtain the activated solution; inoculate the activated solution into MRS liquid medium at an inoculation amount of 5% (v / v), and perform enlarged culture at 37°C to obtain the bacterial solution; centrifuge the bacterial solution at 5000 rpm at 4°C for 10 min, filter to obtain the bacterial cells, and then mix and emulsify with a protective agent (15% skim milk, 5% glycerol, 80% water) with a mass ratio of 1:1, and perform freeze-drying to obtain:

[0061] Bacillus coagulans: BC66 bacterial powder and ATCC7050 bacterial powder (both 1×10 11 CFU / g),

[0062] Bacillus subtilis: ATCC6633 bacterial powder and ATCC10719 bacterial powder (both 1×10 11 CFU / g), Aspergillus niger: ATCC16404 bacterial powder and ATCC10582 bacterial powder (both 2×10 10 CFU / g),

[0063] Saccharomyces cerevisiae: ATCC26603 bacterial powder and ATCC9763 bacterial powder (both 2×10 10 CFU / g),

[0064] Lactobacillus plantarum: LP194 bacterial powder and ATCC8014 bacterial powder (both 4×10 11 CFU / g),

[0065] Lactobacillus acidophilus: LA71 bacterial powder and ATCC4356 bacterial powder (both 2×10 11 CFU / g),

[0066] Lactobacillus buchneri: LBu101 bacterial powder and ATCC339957 bacterial powder (both 2×10 11 CFU / g).

[0067] Preparation Example 1

[0068] Provide a compound microbial agent for chicken manure fermentation and regeneration, which consists of a first compound microbial agent and a second compound microbial agent in independent packages;

[0069] First compound microbial agent: Mix 50 g of Bacillus coagulans BC66 bacterial powder, 125 g of Bacillus subtilis ATCC6633 bacterial powder, 75 g of Aspergillus niger ATCC16404 bacterial powder, 50 g of Saccharomyces cerevisiae ATCC26603 bacterial powder, and 700 g of zeolite powder evenly.

[0070] Second compound microbial agent: Mix 10 g of Lactobacillus plantarum Lp194 bacterial powder, 15 g of Lactobacillus acidophilus LA71 bacterial powder, 15 g of Lactobacillus buchneri LBu101 bacterial powder, and 60 g of zeolite powder evenly.

[0071] Preparation Example 2

[0072] Provide a compound microbial agent for chicken manure fermentation and regeneration, which consists of a first compound microbial agent and a second compound microbial agent in independent packages;

[0073] First compound microbial agent: Mix 25 g of Bacillus coagulans BC66 bacterial powder, 200 g of Bacillus subtilis ATCC10719 bacterial powder, 50 g of Aspergillus niger ATCC10582 bacterial powder, 25 g of Saccharomyces cerevisiae ATCC9763 bacterial powder, and 700 g of zeolite powder evenly.

[0074] Second compound bacterium agent: Mix 5 g of Lactobacillus plantarum Lp194 powder, 20 g of Lactobacillus acidophilus LA71 powder, 15 g of Lactobacillus buchneri LBu101 powder, and 60 g of zeolite powder evenly to obtain it.

[0075] Preparation Example 3

[0076] Provide a compound bacterium agent for chicken manure fermentation and regeneration, which consists of the first compound bacterium agent and the second compound bacterium agent in independent packages; the difference from Preparation Example 1 is only that:

[0077] Replace the Bacillus coagulans BC66 powder with an equal mass of Bacillus coagulans ATCC7050 powder; replace the Lactobacillus plantarum Lp194 powder with an equal mass of Lactobacillus plantarum ATCC8014 powder; replace the Lactobacillus acidophilus LA71 powder with an equal mass of Lactobacillus acidophilus ATCC4356 powder; replace the Lactobacillus buchneri LBu101 powder with an equal mass of ATCC339957 powder. Keep the others unchanged.

[0078] Preparation Example 4

[0079] Provide a compound bacterium agent for chicken manure fermentation and regeneration, which consists of the first compound bacterium agent and the second compound bacterium agent in independent packages; the difference from Preparation Example 1 is only that:

[0080] The first compound bacterium agent lacks Bacillus coagulans BC66 powder, and replace it with an equal mass of Bacillus subtilis ATCC6633 powder. Keep the others unchanged.

[0081] Preparation Example 5

[0082] Provide a compound bacterium agent for chicken manure fermentation and regeneration, which consists of the first compound bacterium agent and the second compound bacterium agent in independent packages; the difference from Preparation Example 1 is only that:

[0083] The first compound bacterium agent lacks Bacillus subtilis ATCC6633 powder, and replace it with an equal mass of Bacillus coagulans BC66 powder. Keep the others unchanged.

[0084] Preparation Example 6

[0085] Provide a compound bacterium agent for chicken manure fermentation and regeneration, which consists of the first compound bacterium agent and the second compound bacterium agent in independent packages; the difference from Preparation Example 1 is only that:

[0086] The second compound bacterium agent lacks Lactobacillus acidophilus LA71 powder, and replace it with an equal mass of Lactobacillus buchneri LBu101 powder. Keep the others unchanged.

[0087] Preparation Example 7

[0088] A composite bacterial agent for chicken manure fermentation regeneration is provided, which is composed of a first composite bacterial agent and a second composite bacterial agent in independent packages; the difference between the composite bacterial agent and the preparation example 1 is only that:

[0089] The second composite bacterial agent lacked Lactobacillus buchneri LBu101 powder, and an equal amount of the powder was replaced with Lactobacillus acidophilus LA71 powder, while the rest remained unchanged.

[0090] Example 1

[0091] Provided is a regenerated feed, which is prepared by the following method:

[0092] (1) Primary fermentation: 700 kg of fresh chicken manure and 300 kg of rice husk were mixed, 200 g of the first composite bacterial agent of Preparation Example 1 was added, the moisture content was adjusted to 55%, and the mixture was evenly mixed and filled into a fermentation tank with a length, width and height of 1 m×1 m×1.2 m. One side was enclosed with a bamboo fence to ensure its air permeability. The top was uncovered, the mixture was turned over once a day, and fermented at 60-70° C. for 10 days.

[0093] (2) Secondary fermentation: 750 kg of the material after primary fermentation was mixed with 500 kg of crushed corn stalks and 75 kg of molasses, 15 g of the second composite bacterial agent of Preparation Example 1 was weighed and dissolved in an appropriate amount of water, and sprayed evenly on the surface of the material during the mixing process, and the moisture content was adjusted to 50%. After mixing evenly, the mixture was packed into a 50 kg plastic bag, sealed and fermented in an anaerobic manner, and placed in a cool place at 35-45° C. for 7 days.

[0094] Example 2-3

[0095] Two regenerated feeds are provided, and the preparation methods thereof differ from those of Example 1 only in that:

[0096] The first composite bacterial agent of Preparation Example 1 was replaced by the first composite bacterial agent of Preparation Examples 2-3 in equal amounts, and the second composite bacterial agent of Preparation Example 1 was replaced by the second composite bacterial agent of Preparation Examples 2-3 in equal amounts.

[0097] Comparative Examples 1-4

[0098] Four kinds of regenerated feeds are provided, and the preparation methods thereof differ from those of Example 1 only in that:

[0099] The first composite bacterial agent of Preparation Example 1 was replaced by the first composite bacterial agent of Preparation Examples 4-7 in equal amounts, and the second composite bacterial agent of Preparation Example 1 was replaced by the second composite bacterial agent of Preparation Examples 4-7 in equal amounts.

[0100] Comparative Example 5

[0101] Provided is a regenerated feed, which is prepared by the following method:

[0102] 700 kg of fresh chicken manure and 300 kg of rice husk were mixed, 200 g of the first composite bacterial agent of Preparation Example 1 was added, the moisture content was adjusted to 55%, and after being mixed evenly, the mixture was filled into a fermentation tank with a length, width and height of 1 m×1 m×1.2 m, one side of which was enclosed with a bamboo fence to ensure its air permeability, the top was uncovered, the mixture was turned over once a day, and fermented at 65° C. for 10 days.

[0103] Comparative Example 6

[0104] Provided is a regenerated feed, which is prepared by the following method:

[0105] Mix 750kg of fresh chicken manure with 500kg of crushed corn stalks and 75kg of molasses, weigh 15g of the second composite bacterial agent of Preparation Example 1 and dissolve it in an appropriate amount of water, spray it evenly on the surface during the material mixing process, and adjust the moisture content to 50%. After mixing evenly, put it into a 50kg plastic bag, seal it for anaerobic fermentation, place it in a cool place, and ferment it at 45°C for 7 days.

[0106] Test Example 1

[0107] The sanitary indexes of the regenerated feeds prepared in Examples 1-3 and Comparative Examples 1-6 were measured. The test standards and test results are shown in Table 1.

[0108] Table 1

[0109]

[0110]

[0111] It can be seen from the test results in Table 1 that, compared with the regenerated feeds of Comparative Examples 1-6, the regenerated feeds obtained by fermentation with the composite bacterial agent of the present invention can effectively kill harmful and toxic substances in chicken manure, thus providing a guarantee for the safety of the regenerated feeds.

[0112] Test Example 2

[0113] The regenerated feeds prepared in Examples 1-3 and Comparative Examples 1-4 were sampled for animal feeding tests, and the test method was as follows: 160 piglets (half male and half female) with similar weight and in the growth stage were selected, with an average weight of 25.13±0.15 kg, and were evenly divided into test groups 1-7 and a control group, with 20 piglets in each group. The control group was fed with a basic diet (see Table 2), and the test groups 1-7 were respectively replaced with an equal amount of the basic diet (see Table 3) by the regenerated feeds prepared in Examples 1-3 and Comparative Examples 1-4 according to the scheme in Table 3, and the test period was 21 days, and the piglets were free to eat during the test.

[0114] Table 2

[0115]

[0116]

[0117] Table 3

[0118] Feeding time Basal diet (%) Fermented chicken manure feed (%) The first week 90 10 The second week 80 20 The third week 70 30

[0119] The average daily feed intake, average daily weight gain, feed-to-meat ratio and diarrhea rate of each group of piglets during the test period were statistically counted as shown in Table 4 to evaluate the effect after replacing the basal diet with the regenerated feed.

[0120] Table 4

[0121]

[0122] It can be seen from the data results in Table 4 that compared with the regenerated feeds of Comparative Examples 1-4, feeding piglets with the regenerated feed obtained by fermenting with the compound bacterium agent involved in the present invention can reduce the feeding cost, improve the growth performance of piglets, promote intestinal health and reduce the diarrhea rate, indicating that the strains in the compound bacterium agent involved in the present invention cooperate with each other to play a role in the above-mentioned effects. And the effect of the group of Example 1 is better than that of Example 3, indicating that when Bacillus coagulans, Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus buchneri are selected with BC66 strain, LP194 strain and LA71 strain, the prepared regenerated feed has better effects in terms of animal palatability, improvement of animal intestines, promotion of animal growth performance and reduction of diarrhea rate.

[0123] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of each raw material of the product of the present invention, addition of auxiliary components, selection of specific methods, etc. all fall within the protection scope and public scope of the present invention.

[0124] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0125] In addition, it should be noted that in the various specific technical features described in the above specific embodiments, they can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not separately describe various possible combination methods.

Claims

1. A composite microbial agent for chicken manure fermentation and regeneration, characterized in that, The composite microbial agent for chicken manure fermentation and regeneration consists of a first composite microbial agent and a second composite microbial agent in independent packages; The strains in the first composite microbial agent consist of Bacillus coagulans, Bacillus subtilis, Aspergillus niger, and Saccharomyces cerevisiae; the strains in the second composite microbial agent consist of Lactobacillus plantarum, Lactobacillus acidophilus, and Lentilactobacillus buchneri.

2. The composite microbial agent for chicken manure fermentation and regeneration according to claim 1, wherein The viable count ratio of Bacillus coagulans, Bacillus subtilis, Aspergillus niger, and Saccharomyces cerevisiae in the first composite microbial agent is (0.5 - 2):(1 - 6):(1 - 3):(0.5 - 4); The viable count ratio of Lactobacillus plantarum, Lactobacillus acidophilus, and Lentilactobacillus buchneri in the second composite microbial agent is (0.1 - 2):(0.2 - 3):(0.2 - 3).

3. The composite microbial agent for chicken manure fermentation and regeneration according to claim 1 or 2, characterized in that, The total viable count in the first compound bacterial agent is not less than 2×10 10 CFU / g or 2×10 10 CFU / mL; The total viable count in the second compound bacterium agent is not less than 1×10 11 CFU / g or 1×10 11 CFU / mL.

4. The composite microbial inoculum for chicken manure fermentation and regeneration according to any one of claims 1-3, characterized in that The Bacillus coagulans is the Bacillus coagulans BC66 strain with the preservation number of CGMCC 21879; Preferably, the Lactobacillus plantarum is the Lactobacillus plantarum LP194 strain with the preservation number of CGMCC 21766; Preferably, the Lactobacillus acidophilus is the Lactobacillus acidophilus LA71 strain with the preservation number of CGMCC 21765; Preferably, the Lentilactobacillus buchneri is the Lentilactobacillus buchneri LBu101 strain with the preservation number of GDMCC No:63094.

5. The composite microbial agent for chicken manure fermentation and regeneration according to any one of claims 1-4, characterized in that, The first composite microbial agent and the second composite microbial agent also include a carrier; Preferably, the first composite microbial agent and the second composite microbial agent also include a cell protectant; Preferably, the first composite microbial agent and the second composite microbial agent also include a nutrient component; Preferably, the dosage forms of the first composite microbial agent and the second composite microbial agent are independently liquid agents or powders.

6. Use of the composite microbial agent according to any one of claims 1 - 5 in the preparation of chicken manure fermentation and regeneration feed.

7. A preparation method of fermented and recycled chicken manure feed, characterized in that, The preparation method is obtained by fermenting chicken manure with the composite microbial agent according to any one of claims 1 - 5, and includes the following steps: (1) Inoculate the first composite microbial agent into the first material for aerobic fermentation to obtain a primary fermentation product; (2) Inoculate the second composite microbial agent into the second material for anaerobic fermentation to obtain the chicken manure fermentation and regeneration feed; The first material contains chicken manure; the second material contains the primary fermentation product.

8. The preparation method according to claim 7, wherein The inoculation amount of the first composite microbial agent is 0.02% - 2%, and the inoculation amount of the second composite microbial agent is 0.001% - 1%; Preferably, the first material also contains an auxiliary material, and the auxiliary material includes plant fiber; Preferably, the mass ratio of the first material to the auxiliary material is (6 - 7):(3 - 4); Preferably, the second material also contains an auxiliary material, and the auxiliary material includes plant fiber; Preferably, the mass ratio of the second material to the auxiliary material is (4 - 8):(2 - 6); Preferably, the second material also contains an additionally added carbon source; Preferably, the carbon source is 2 - 10% of the mass of the second material.

9. The preparation method according to claim 7 or 8, characterized in that, The moisture content of the first material is 55-60%, and the moisture content of the second material is 50-60%.

10. The preparation method according to any one of claims 7-9, characterized in that, The aerobic fermentation is carried out at 55-70 °C for 7-10 days; The anaerobic fermentation is carried out at 30-45 °C for 7-10 days.

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