Lactobacillussp.W2 strain and application thereof

A technology of lactobacillus and phenyl lactic acid, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve problems such as difficult control of product quality, serious pollution of the environment, complex technology, etc., to achieve easy cultivation and preservation, and inhibition of spoilage bacteria , good growth effect

Inactive Publication Date: 2010-12-08
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems of the chemical method are that the optical purity of the product is not high enough, the technology is comp

Method used

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  • Lactobacillussp.W2 strain and application thereof
  • Lactobacillussp.W2 strain and application thereof
  • Lactobacillussp.W2 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This example demonstrates that Lactobacillus Lactobacillus Screening and purification identification method of sp.W2.

[0027] 1. Breeding of strains:

[0028] Accurately weigh 25 g of yogurt dregs (purchased from Zhumuqin Banner, Xilinhot, Inner Mongolia), and add them into a triangular flask containing 225 mL of MRS liquid medium under aseptic conditions, and enrich and culture at 35°C for 24 h. Draw 1 mL of yogurt dregs culture solution for gradient dilution, draw 1 mL of enriched culture solution with different dilutions and place it on the solidified sterile isolation medium, and spread the bacteria solution evenly on the whole with a sterile coating stick. On the surface of the plate, incubate at 35°C for 2 to 3 days, pick out the colonies visible to the naked eye grown in the culture medium, separate and culture them by streaking on the BCP plate medium respectively, and pick the yellow colonies in the culture medium around the colonies for further purification...

Embodiment 2

[0036] This example demonstrates that Lactobacillus Lactobacillus Identification method of sp.W2.

[0037] 1. Identification of strain W2:

[0038] Determination and comparative analysis of the sequence of the 16S rDNA part of W2, identified as Lactobacillus Lactobacillus sp., identified and deposited under the Microorganism Deposit Procedure, and classified as Lactobacillus Lactobacillus sp.W2, its deposit registration number is CCTCC NO: M 209318.

[0039] 2. Lactobacillus Lactobacillus Features of sp.W2:

[0040] Morphological features:

[0041] Lactobacillus Lactobacillus The sp.W2 strain was cultivated on the MRS solid medium at 35°C for 48 hours, forming obvious colonies with a diameter between 0.5 and 2.0 mm, round, needle-shaped in the medium, neat edges, milky white, opaque, and moist on the surface Smooth and non-pigmented. Observed under a microscope, Gram staining is positive, no movement, short rod-shaped cells, 0.29-0.45 mm×1.58-4.20 mm, no sporulati...

Embodiment 3

[0045] This example demonstrates that Lactobacillus Lactobacillus Application of sp.W2 in fermentative production of phenyllactic acid.

[0046] Will Lactobacillus sp.W2 was inoculated into MRS liquid medium for fermentation, cultured at 37°C for 17 hours, then the seed culture solution was added to the fermentation medium at 5% inoculum, and cultured at 35-37°C for 20-24 hours. The obtained fermentation broth was centrifuged, and the precipitate was discarded. The supernatant was extracted by adding 2 times the volume of ethyl acetate, evaporated and concentrated under reduced pressure to obtain the crude product of phenyllactic acid, which was then dissolved in 10 mL of 0.05% trifluoroacetic acid aqueous solution. The content of phenyllactic acid was determined by liquid chromatography (HPLC), and the content of phenyllactic acid obtained could be as high as 1.038 g / L.

[0047] The HPLC determination conditions are as follows: the mobile phase is a mixture of 0.05% trifl...

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Abstract

The invention relates to Lactobacillussp.W2. The preservation number of the Lactobacillussp.W2 is CCTCCM209318. The invention also relates to application of the Lactobacillussp.W2 in biologically transforming phenylpyruvic acid substrate and extracting phenyllactic acid from fermentation solution by using ethyl acetate. The Lactobacillussp.W2 is from the traditional fermented food, belongs to an acknowledged safe strain and can be applied to food; the strain grows well on an MRS culture medium and is easily cultured and stored; and the content of the phenyllactic acid obtained by fermentation can reach 1.038 grams per liter, and the phenyllactic acid has strong capability of inhibiting putrefactive bacteria in the food.

Description

technical field [0001] The invention relates to a strain of Lactobacillus Lactobacillus The sp.W2 bacterial strain and its application in the fermentative production of phenyl lactic acid belong to the technology in the field of industrial microorganisms. Background technique [0002] β -Phenyllactic acid, also known as 3-phenyllactic acid or 2-hydroxy-3-phenylpropionic acid (Phenyllactic acid, referred to as PLA), has a relative molecular mass of 166 and a melting point of 121-125°C. It is odorless and resistant to acid and heat. It is stable and will not be destroyed when heated at 121°C. It naturally exists in honey and is non-toxic to human or animal cells; its second carbon atom is a chiral carbon atom, so there are two enantiomers R-(+ )-3-phenyllactic acid (D-(+)-3-phenyllactic acid) and S-(-)-3-phenyllactic acid (L-(-)-3-phenyllactic acid). β -Phenyl lactic acid is a new type of antibacterial substance with a broad antibacterial spectrum, and its antibacterial pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/42C12R1/225
Inventor 王立梅郑丽雪梅艳珍齐斌
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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