Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbial cell cryogel, preparation method and application thereof

A microbial cell and crystal gel technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor stability of whole-cell catalysts, impossibility of industrial application, high process cost, etc., and achieve large-scale transformation and preparation Easy to implement, good biocompatibility and safety, simple preparation method

Pending Publication Date: 2018-08-10
ZHEJIANG UNIV OF TECH
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of synthesizing phenyllactic acid with biological enzymes is costly and difficult for industrialization; the synthesis of phenyllactic acid by using whole-cell catalyst microbial conversion method can reduce the cost and become the main development direction of phenyllactic acid synthesis
However, conventional whole-cell catalysts are poorly stable and difficult to reuse
The whole-cell catalyst immobilized by conventional carriers has small pore size, high mass transfer resistance, low cell concentration, serious loss, low conversion activity rate, and cannot be applied industrially.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 0.242g of Lactobacillus casei cells, 0.0121g of polyvinyl glutaraldehyde crosslinking agent and 0.29g of water were mixed uniformly to prepare a suspension, and the phase change crystallizing and crosslinking were carried out at a temperature of ‒10~‒40℃. After 150 hours of reaction, the microbial cell gel was obtained. After scanning electron microscope, flow pressure test and analysis and calculation, its pore size is 1-15 µm, porosity is 53.1%, and water permeability is 1.8×10 -13 m 2 , It has good elasticity in water. In 100 mM phosphate buffer, 8.3 g / L of the key substrate phenylpyruvate and 14 g / L of the auxiliary substrate glucose were added, and the obtained microbial cell gel was used as the biocatalyst, and the amount and the quality of the phenylpyruvate substrate The ratio is 13:1, biotransformation is carried out, and phenyllactic acid is synthesized. After 8 hours of conversion reaction at 35℃ and 75 rpm, samples were taken from the conversion solution, c...

Embodiment 2

[0022] 0.0813g of Lactobacillus casei cells, 0.016g of polyvinyl glutaraldehyde crosslinking agent and 0.48g of water were mixed uniformly to prepare a suspension, which was subjected to phase change crystallization and pore formation and crosslinking at a temperature of ‒10~-40℃ React for 170 h to obtain microbial cell gel. After scanning electron microscope, flow pressure test and analysis and calculation, its pore diameter is 1-10 µm, porosity is 36.9%, and water permeability is 1×10 -15 m 2 , It has good elasticity in water. In 100 mM phosphate buffer, add 2g / L of substrate phenylpyruvate and 14g / L of glucose, and use the obtained whole microbial cell crystal gel as the biocatalyst. The ratio of the amount to the mass of phenylpyruvate substrate is 40:1 , Biotransformation, synthesis of phenyllactic acid. After 8 hours of conversion reaction at 35℃ and 75 rpm, samples were taken from the conversion solution, centrifuged and the supernatant was taken for detection and anal...

Embodiment 3

[0024] Mix 0.0024g of Lactobacillus casei cells, 0.0121g of polyvinyl glutaraldehyde crosslinking agent and 0.29g of water uniformly to prepare a suspension, and conduct phase change crystallization and crosslinking at a temperature of ‒10~‒40℃ After 70 hours of reaction, the whole microbial cell crystal gel is obtained, and the water permeability is 3×10 -13 m 2 , It has good elasticity in water. After scanning electron microscopy, flow pressure test and analysis and calculation, its pore diameter is 1-50 µm, and its porosity is 95.2%. In 100 mM phosphate buffer, 8.3 g / L of the key substrate phenylpyruvate and 14 g / L of the auxiliary substrate glucose were added, and the obtained microbial cell monolith gel was used as the biocatalyst. The dosage was the same as that of the phenylpyruvate substrate. The mass ratio is 0.1:1, biotransformation is carried out, and phenyllactic acid is synthesized. After 8 hours of conversion reaction at 35℃ and 75 rpm, samples were taken from t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a microbial cell cryogel for conversion synthesis of phenyllactic acid, a preparation method and application thereof. The microbial cell cryogel is an integral porous material,the matrix skeleton is a cross-linked Lactobacillus casei microbial cell, the pore size ranges from 1 to 50 micrometers, the porosity is 36.9-95.2%, and the water permeability is 1*10<-15>-3*10<-13>m<2>. Serving as a biocatalyst, the cryogel can achieve conversion synthesis of a phenylpyruvic acid key substrate into phenyllactic acid, and the maximum conversion yield can reach 75%. The microbialcell integral cryogel provided by the invention has the advantages of large pore size, high porosity, fast substrate and product mass transfer, good bio-safety, reusability, simple preparation method, and low cost, and has broad application prospects in the biochemical synthesis field.

Description

Technical field [0001] The invention belongs to the field of biochemical industry, and specifically relates to a microbial cell crystal gel and a preparation method and application thereof. It can be used as a biocatalyst for the conversion and synthesis of phenyllactic acid. Background technique [0002] Phenyllactic acid is a new type of antibacterial agent with broad-spectrum antibacterial properties against Gram-negative bacteria, Gram-negative bacteria and some fungi. It is also a key monomer that can be used to synthesize new polymer materials of polyphenyllactic acid. It can also be used as an intermediate for the synthesis of new drugs, and has important uses in many fields such as chemical engineering, biology, food, materials, and medicine. Due to the complex route of chemical synthesis of phenyllactic acid and serious environmental pollution problems, biological methods have become the current research direction at home and abroad. [0003] However, the biosynthesis of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/42C12R1/245
CPCC12N1/20C12P7/42
Inventor 贠军贤许娜潘曼曼张颂红姚善泾
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com