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Quality control product for urine organic acid filter paper and preparation method thereof

A technology of organic acid and filter paper, applied in the field of analysis and detection, can solve the problems of complex chemical composition, instability, harsh storage conditions, etc., and achieve the effect of simple operation, low cost and moderate concentration

Active Publication Date: 2017-08-04
深圳金域医学检验实验室
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the chemical composition of urine is very complex and unstable
According to literature research, the storage conditions of urine are very harsh, and it is necessary to add preservatives and freeze at -70°C. Although bacterial activity can be inhibited under frozen conditions, problems such as compound decomposition and bacterial reproduction are still inevitable.
And due to the large quantity of quality control preparations, storage space is also an issue that needs to be considered
In addition, there is currently no commercial urine organic acid filter paper quality control that can be used for clinical laboratory testing, so there is a need for a preparation method that is not only convenient for storage, but also stable as a quality control product for quality monitoring

Method used

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  • Quality control product for urine organic acid filter paper and preparation method thereof
  • Quality control product for urine organic acid filter paper and preparation method thereof
  • Quality control product for urine organic acid filter paper and preparation method thereof

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Embodiment Construction

[0033]In order to facilitate the understanding of the present invention, the following will describe the present invention more fully. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0035] The preparation method of the urine organic acid filter paper quality control pr...

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Abstract

The invention relates to a quality control product for urine organic acid filter paper and a preparation method thereof. The preparation method comprises the following steps: (1) preparing an organic acid standard liquid: separately preparing standard solutions of methylmalonic acid, glutaric acid, isoamyl glycine, phenylpyruvic acid, orotic acid and 4-hydroyl phenyllactic acid; (2) preparing a working liquid: by taking urine as a solvent, adding the organic acid standard liquid to obtain the working liquid; and (3) dropwise adding the working liquid onto the filter paper, and airing the filter paper to obtain the quality control product for the urine organic acid filter paper. The quality control product for the urine organic acid filter paper prepared by the preparation method provided by the invention can be stored for at least two months under a condition of 20 DEG C below. The uniformity and stability of the urine organic acid filter paper taken out to restore the constant temperature under the condition of 20 DEG C below can meet the requirements on the quality control product.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to a method for preparing a urine organic acid filter paper quality control product. Background technique [0002] Hereditary metabolic diseases refer to a class of genetic diseases with physiological and metabolic defects, including metabolic macromolecular diseases: including lysosomal storage diseases (more than 30 kinds of diseases), mitochondrial diseases, etc., metabolic small molecular diseases: amino acids, Organic acids, fatty acids, etc. The clinical manifestations of children vary greatly among individuals, and clinical diagnosis is difficult, and it is easy to be missed or misdiagnosed. If not diagnosed and treated in time, the mortality rate is very high. Therefore, early diagnosis and reasonable treatment are the key to saving patients' lives. [0003] As one of the important metabolites of the human body, urine is a very important and key biological sample in ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/02G01N30/06G01N2030/042
Inventor 吴婉琼佘旭辉李维李卓阳倪周陈秀如赵蓓蓓程雅婷陈静宜吴华顺
Owner 深圳金域医学检验实验室
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