Application of methylmalonic acid in preparation of nematode pesticide

Abstract

The invention relates to the technical field of agricultural microbiology, and specifically relates to an application of methylmalonic acid in preparation of a nematode pesticide. Through determination of the fatality rate of meloidogyne incognita and caenorhabditis elegans, it finds that the methylmalonic acid has a good insecticidal effect on nematodes; LC50 of the methylmalonic acid to the meloidogyne incognita and the caenorhabditis elegans is separately 13.11 and 1.20; and after the methylmalonic acid and betaine are compounded, the LC50 of the methylmalonic acid to the meloidogyne incognita and the caenorhabditis elegans is separately 2.85 and 0.27; and at the same time, the methylmalonic acid has an inhibition effect on ralstonia solanacearum and erwinia carotovora. The preparationprovides new selectivity for the preparation of biocontrol preparations of meloidogyne.

Description

technical field

[0001] The invention belongs to the technical field of agricultural microbiology, and in particular relates to the application of methylmalonic acid in the preparation of nematode insecticides. Background technique

[0002] At present, there are more than 3,000 kinds of nematodes that are harmful to plants, and more than 40 kinds have been found in my country. One of the nematodes that is most harmful to crops is root-knot nematode. Root-knot nematodes are tiny and live in plants or in the soil. They will be transferred with rain, labor operations and diseased plants, making it extremely difficult to control. According to incomplete estimates, the global economic loss caused by nematodes is about 80 billion. In tropical and subtropical areas where root-knot nematodes are severely infested, crop yields can be reduced by more than 80%.

[0003] Root-knot nematode (Meloidogyne) is a dioecious, highly specialized omnivorous plant pathogenic nematode, and its li...

Examples

Embodiment 1

[0025] Structural verification of nematicidal methylmalonic acid

[0026] 1. Methylmalonic acid was purchased from Sigma.

[0027] 2. Nuclear Magnetic Resonance (NMR)

[0028] The structure of methylmalonic acid was detected by nuclear magnetic resonance: using deuterium water (D2O) as solvent, the hydrogen spectrum (H NMR) and carbon spectrum ( 13 C NMR), Table 1 is the NMR data.

[0029] Table 1 Two-dimensional NMR data of methylmalonic acid

[0030]

Embodiment 2

[0032] The application of methylmalonic acid in the preparation of root-knot nematode insecticide, the application process is as follows:

[0033] The root-knot nematodes infected with root-knot nematodes were picked from the roots of tomatoes, and egg masses were placed in a 96-well plate to incubate at 20°C to observe the hatching of root-knot nematodes. Rinse the nematodes with sterile water, add 20 μL of 1 mg / mL methylmalonic acid aqueous solution to the 96-well plate, and add pure water at the same time, adjust the final concentration of methylmalonic acid in the orifice plate to 1000, 100, 25, 16.67, 12.5, 10, 1 μg / mL, 40 nematodes were added to each well, the total bioassay system was 200 μL, pure water was used as a control, and each group was repeated 3 times. Cultured in a 16°C incubator, the LC of methylmalonic acid on Meloidogyne incognita 50 The value is shown in Table 1, which is 13.11 μg / mL.

[0034] Table 1 Insecticidal activity of methylmalonic acid against ...

Embodiment 3

[0039] The application of methylmalonic acid in the preparation of Caenorhabditis elegans insecticide, the application process is as follows:

[0040] Add 20 μL of 1 mg / mL methylmalonic acid aqueous solution to the 96-well plate, and add purified water at the same time, adjust the final concentration of methylmalonic acid in the orifice plate to 1000, 100, 25, 16.67, 12.5, 10, 1 μg / mL, 40 nematodes were added to each well, the total bioassay system was 200 μL, pure water was used as a control, and each group was repeated 3 times. The LC of Caenorhabditis elegans by methylmalonic acid was cultured in a 16°C incubator 50 The value is shown in Table 2, which is 1.20 μg / mL.

[0041] Table 2 Insecticidal activity of methylmalonic acid against Caenorhabditis elegans

[0042]

[0043]

[0044] Using the above method, the applicant tested the insecticidal effect of betaine, and the LC of betaine to Caenorhabditis elegans was tested. 50 It was 14.75 μg / ml.

technical field

[0001] The invention belongs to the technical field of agricultural microbiology, and in particular relates to the application of methylmalonic acid in the preparation of nematode insecticides. Background technique

[0002] At present, there are more than 3,000 kinds of nematodes that are harmful to plants, and more than 40 kinds have been found in my country. One of the nematodes that is most harmful to crops is root-knot nematode. Root-knot nematodes are tiny and live in plants or in the soil. They will be transferred with rain, labor operations and diseased plants, making it extremely difficult to control. According to incomplete estimates, the global economic loss caused by nematodes is about 80 billion. In tropical and subtropical areas where root-knot nematodes are severely infested, crop yields can be reduced by more than 80%.

[0003] Root-knot nematode (Meloidogyne) is a dioecious, highly specialized omnivorous plant pathogenic nematode, and its li...

Examples

Embodiment 1

[0025] Structural verification of nematicidal methylmalonic acid

[0026] 1. Methylmalonic acid was purchased from Sigma.

[0027] 2. Nuclear Magnetic Resonance (NMR)

[0028] The structure of methylmalonic acid was detected by nuclear magnetic resonance: using deuterium water (D2O) as solvent, the hydrogen spectrum (H NMR) and carbon spectrum ( 13 C NMR), Table 1 is the NMR data.

[0029] Table 1 Two-dimensional NMR data of methylmalonic acid

[0030]

Embodiment 2

[0032] The application of methylmalonic acid in the preparation of root-knot nematode insecticide, the application process is as follows:

[0033] The root-knot nematodes infected with root-knot nematodes were picked from the roots of tomatoes, and egg masses were placed in a 96-well plate to incubate at 20°C to observe the hatching of root-knot nematodes. Rinse the nematodes with sterile water, add 20 μL of 1 mg / mL methylmalonic acid aqueous solution to the 96-well plate, and add pure water at the same time, adjust the final concentration of methylmalonic acid in the orifice plate to 1000, 100, 25, 16.67, 12.5, 10, 1 μg / mL, 40 nematodes were added to each well, the total bioassay system was 200 μL, pure water was used as a control, and each group was repeated 3 times. Cultured in a 16°C incubator, the LC of methylmalonic acid on Meloidogyne incognita 50 The value is shown in Table 1, which is 13.11 μg / mL.

[0034] Table 1 Insecticidal activity of methylmalonic acid against ...

Embodiment 3

[0039] The application of methylmalonic acid in the preparation of Caenorhabditis elegans insecticide, the application process is as follows:

[0040] Add 20 μL of 1 mg / mL methylmalonic acid aqueous solution to the 96-well plate, and add purified water at the same time, adjust the final concentration of methylmalonic acid in the orifice plate to 1000, 100, 25, 16.67, 12.5, 10, 1 μg / mL, 40 nematodes were added to each well, the total bioassay system was 200 μL, pure water was used as a control, and each group was repeated 3 times. The LC of Caenorhabditis elegans by methylmalonic acid was cultured in a 16°C incubator 50 The value is shown in Table 2, which is 1.20 μg / mL.

[0041] Table 2 Insecticidal activity of methylmalonic acid against Caenorhabditis elegans

[0042]

[0043]

[0044] Using the above method, the applicant tested the insecticidal effect of betaine, and the LC of betaine to Caenorhabditis elegans was tested. 50 It was 14.75 μg / ml.

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