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Dried blood spot quantitative collection device and method

A collection device and quantitative collection technology, applied in the field of dry blood spot detection, can solve the problems of uneven blood diffusion, difficult sampling, cumbersome extraction process, etc., and achieve the effects of saving time and consumables, accurate detection results, and high extraction recovery rate

Pending Publication Date: 2020-07-31
WUXI DIAGNOSTICS LAB SHANGHAI CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] On the other hand, there are also some problems and deficiencies in the dry blood spot detection method, which limit its further wide application.
These problems and deficiencies include: it is impossible to check the blood source and sampling time during self-service sampling; due to the limitations of collection conditions and collection operations, despite the guidance of training and instructions, many samples are still unqualified; patients are afraid of self-service skin puncture Psychological; due to the small sample volume, some low-concentration analytes cannot be effectively detected
When the capillary is transferred, the slender capillary is easy to shake when held, which makes sampling difficult. The transfer process takes a long time, and the filter paper is easily scratched due to displacement at the contact point with the filter paper, resulting in uneven blood diffusion.
The existing dried blood spot sample collection tools are low in integration, the consumables are scattered, and the filter paper is often exposed, so the probability of sample contamination is very high
The extraction process of the dried blood spot sample in the existing blood collection card is cumbersome, and it is necessary to use a special tool to lay a blood spot with a fixed area in the center of the sample. The operation is time-consuming and laborious, and there is a possibility of cross-contamination

Method used

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  • Dried blood spot quantitative collection device and method
  • Dried blood spot quantitative collection device and method
  • Dried blood spot quantitative collection device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A dry blood spot quantitative collection device, such as Figure 1~3 As shown, it includes: sample collection tube 1, quantitative blood collection tube 2 and sample collection filter paper 3. Wherein, the sample collection tube 1 is composed of a tube body 1a and a tube cover 1b. The tube body 1a is a straight tube with opposite first and second ends, wherein the first end is a closed tube bottom 1a1, and the second end is an open nozzle 1a2. The tube cap 1b is used to close the tube opening 1a2. Wherein, the quantitative blood collection tube 2 includes a fixing part 2a and a quantitative capillary 2b fixed on the fixing part 2a. The fixing part 2a is engaged with the mouth 1a2 of the sample collection tube 1, and the quantitative capillary 2b extends into the tube; the quantitative capillary 2b has a blood sampling end 2b1 for blood collection, and the blood sampling end 2b1 is exposed outside the fixing part 2a. Wherein, the sample collection filter paper 3 is ar...

Embodiment 2

[0100] Example 2: Collection of fingertip blood for therapeutic drug monitoring (TDM)

[0101] This method can be used to collect fingertip peripheral blood to make dried blood film samples. Compared with venous sampling, dried blood film samples have unique advantages such as less blood collection volume, stable samples, easy storage and transportation, and sampling without time and space constraints. For some therapeutic drug monitoring (TDM), it is necessary to collect peak and trough concentrations at specific times, or to sample frequently in a short period of time, and peripheral blood dried blood samples can meet clinical needs. The method is simple to operate and has high sample quality, and can be extended to home self-service sampling after training.

[0102] Such as Figure 4 As shown, when it is used to collect fingertip blood, the operation steps are as follows:

[0103] 1) Paste the sample source information on the outside of the sample collection tube, open t...

Embodiment 3

[0124] Example 3: Collection of human whole blood for nucleic acid detection

[0125] In the method, a venous whole blood sample is loaded onto a blood sample collection device, and nucleic acid is extracted for gene mutation detection.

[0126] A. Sample Collection

[0127] (1). Paste the sample source information on the outside of the sample collection tube, open the cover of the sample collection tube, and take out the quantitative blood collection tube for later use;

[0128] (2). Follow the standard operation of venous blood collection to collect blood samples.

[0129] (3). Use a pipette to add the venous blood sample onto the filter paper of the dry blood collection tube until the blood sample is completely dry. Transport or store under proper temperature conditions.

[0130] B. Nucleic acid extraction

[0131] After the dried blood spot samples were collected, nucleic acid was extracted from the samples using QIAgene's QIAamp DNA mini nucleic acid extraction kit (C...

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Abstract

The invention provides a dried blood spot quantitative collection device. The dried blood spot quantitative collection device comprises a sample collection tube, sample collection filter paper and a quantitative blood collection tube, wherein the sample collection tube comprises a tube body and a tube cover; the tube body is a straight tube, and the tube cover is used for sealing the tube opening.The quantitative blood collection tube comprises a quantitative capillary tube and a fixing piece; the quantitative capillary tube is fixed on the fixing piece and is provided with a blood sampling end which is used for sampling blood and is exposed out of the fixing piece; the fixing piece is clamped in the tube opening, and the quantitative capillary tube extends into the tube body; the samplecollection filter paper is arranged in the tube body, and the blood sampling end abuts against the sample collection filter paper. The invention further provides a method for collecting the dried blood spots. The integrated blood collecting device design is adopted, sample collection, drying, transportation, storage and extraction can be achieved in the same device, sample pollution is avoided, and the accuracy of a dried blood spot quantitative detection result is improved.

Description

technical field [0001] The invention relates to the technical field of dry blood spot detection in biotechnology, in particular to a dry blood spot quantitative collection device and method. Background technique [0002] Dried Blood Spot (DBS) detection technology is a method in which a biological sample (usually whole blood) is dropped onto a filter paper sheet for dry storage, and then extracted to detect analytes in the dry spot sample. It is used for qualitative analysis and detection of nucleic acid genetic material. Because of its advantages of convenient collection, less blood collection, and easy storage and transportation of samples, it has been used in newborn screening for phenylketonuria since the 1960s. At present, the most mature field of application of dry blood spot detection is still the screening of genetic metabolic diseases in newborns. At the same time, its application is gradually expanding to the fields of endogenous biomarker detection and therapeuti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N1/14G01N30/06G01N30/72A61B5/151A61B5/15C12Q1/6851G01N33/68
CPCG01N1/14G01N30/02G01N30/06G01N30/72A61B5/151A61B5/150022A61B5/150343A61B5/150755C12Q1/6851G01N33/6854G01N2001/149G01N2030/027G01N2030/062C12Q2531/113
Inventor 杭红张云李宇张向辉赵伟洁
Owner WUXI DIAGNOSTICS LAB SHANGHAI CO LTD
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