Ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry screening method for mycotoxin in milk and dairy product

An ultra-high performance liquid phase and ion trap mass spectrometer technology is applied in the field of ultra-high performance liquid chromatography-quadrupole electrostatic field orbital ion trap mass spectrometry screening, which can solve the problems of high cost of materials and equipment, complicated pretreatment operations, and the like, Accurate qualitative and quantitative results, saving analysis time, and high resolution

Inactive Publication Date: 2017-03-22
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology

[0006] However, at present, there are few high-throughput and rapid detection methods for exogenous risk substances in milk and dairy products. The pre-treatment operations are complicated, and the cost of materials and equipment is high. They are limited to the rapid detection of several or one type of compounds.

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  • Ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry screening method for mycotoxin in milk and dairy product
  • Ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry screening method for mycotoxin in milk and dairy product
  • Ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry screening method for mycotoxin in milk and dairy product

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Embodiment Construction

[0037] The invention provides an ultra-high performance liquid chromatography-quadrupole electrostatic field orbital ion trap mass spectrometry screening method for mycotoxins in milk and dairy products, which is characterized in that the equipment used in the method includes ultra-high performance liquid chromatography- Quadrupole electrostatic field orbital ion trap mass spectrometer. Include the following steps:

[0038] First, prepare matrix solution and standard solution; use the optimized QuEChERS method to extract and clean up milk and dairy product samples;

[0039] Secondly, ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry (Q-Orbitrap) was used for screening, using electrospray ionization source (ESI) and full scan / data dependent acquisition (Full MS / dd-MS 2 ), full scan / variable data-independent acquisition (Full MS / vDIA), full scan / full ion fragmentation acquisition (Full MS / AIF) three scan modes to obtain c...

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Abstract

The invention discloses an ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry screening method for mycotoxin in milk and dairy products. The method is characterized in that a sample is pre-treated by an optimized QuEChERS method; a ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry is used for screening, full-scanning/data-independent collection, full-scanning/variable data non-independent collection, and full-scanning/ion fragmentation collection scanning mode is employed, a positive ion or negative ion mode is selected for scanning to obtain complete first-order and second-order spectrums; Exact Finder 2.5 software is used for processing the obtained first-order and second-order spectrums, and the compound information is extracted. Compared the information in spectrum database and the screening result of the to-be-measured objects, composition of mycotoxin in the milk and dairy products can be confirmed. The mass spectrum apparatus has the advantages of high resolution, accurate qualitative and quantification result, high sensitivity, and high quality precision, and can be used for analyzing the complex matrix.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of food safety detection, in particular to an ultra-high performance liquid chromatography-quadrupole electrostatic field orbital ion trap mass spectrometry screening method for mycotoxins in milk and dairy products. [0003] 【Background technique】 [0004] Milk and dairy products are one of the most popular foods today and are loved for their rich nutritional value. With the improvement of living standards, people pay more and more attention to the safety of milk and dairy products. Exogenous risk substances are an important aspect that affects the safety of milk and dairy products. More and more attention has been paid to it. As an exogenous substance, mycotoxins are extremely harmful to humans and animals. Mycotoxins include aflatoxins, ochratoxins, zearalenones, fumonisins, deoxynivalenols, and ergot alkaloids. [0005] Mycotoxins in milk and dairy products include protoxins in feed and their m...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/7266
Inventor 贾玮张焱茜
Owner SHAANXI UNIV OF SCI & TECH
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