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Test paper for rapidly detecting immunochromatography of cadmium ion colloidal gold and preparation method and application thereof

A technology for detecting test strips and immunochromatography, which is applied in the directions of measuring devices, analytical materials, instruments, etc., to achieve the effects of low cost, economical efficiency, and convenient use.

Inactive Publication Date: 2009-11-11
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the rapid detection method of cadmium ion colloidal gold immunochromatography at home and abroad, and there is no corresponding supply of colloidal gold detection test strips in the market. Therefore, the rapid detection of heavy metal cadmium by colloidal gold immunochromatography with independent intellectual property rights method is important

Method used

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  • Test paper for rapidly detecting immunochromatography of cadmium ion colloidal gold and preparation method and application thereof
  • Test paper for rapidly detecting immunochromatography of cadmium ion colloidal gold and preparation method and application thereof
  • Test paper for rapidly detecting immunochromatography of cadmium ion colloidal gold and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 Fish sample detection

[0051] 1. Preparation of cadmium ion colloidal gold rapid detection test strip:

[0052] (1) Preparation of anti-cadmium ion monoclonal antibody: Utilize the bifunctional chelating agent iEDTA to adopt routine operation, and Cd 2+ Preparation of immune antigen Cd by coupling with hemocyanin (KLH) 2+ -iEDTA-KLH, then immunized BALB / c mice, and prepared anti-cadmium ion monoclonal antibody by conventional hybridoma technology;

[0053] (2) Preparation of the complete antigen Cd-iEDTA-BSA of heavy metal cadmium: Weigh an appropriate amount of CdCl 2 Dissolve to make solution A; weigh an appropriate amount of iEDTA to dissolve to make solution B; take appropriate amount of BSA to dissolve to make solution C; take solution A and add it to solution B drop by drop, mix it upside down, and then add it to solution C drop by drop , placed on a shaker, and oscillated to obtain the coupling solution; collect the coupling solution, move it to ...

Embodiment 2

[0067] Example 2 Detection of Environmental Water Body Samples

[0068] 1. The present embodiment adopts the obtained cadmium ion colloidal gold rapid detection test strip prepared in embodiment 1.

[0069] 2. Sample pretreatment: Take 20mL of shaken water sample, transfer it into a 50mL polytetrafluoroethylene beaker, heat the beaker on an electric furnace with an asbestos net in the fume hood, and add 1ml of nitric acid. When it is concentrated to about 2ml, take it off and cool it, and add 2ml of nitric acid and 0.8ml of perchloric acid along the wall of the cup. Continue heating and digestion. After the solution is clear, add a little triple distilled water, heat to boil, and drive away chlorine and nitrogen oxides. Dissolve it in triple distilled water, filter it into a beaker, adjust the pH to 7.4 with NaOH solution, and constant volume with a 50ml volumetric flask to prepare the sample loading solution to be tested.

[0070] 3. Sample testing

[0071] (1) Add 100mM E...

Embodiment 3

[0075] Example 3 Environmental Soil Sample Detection

[0076] 1. The present embodiment adopts the obtained cadmium ion colloidal gold rapid detection test strip prepared in embodiment 1.

[0077] 2. Sample pretreatment: Accurately weigh 1.2g of soil sample in a 50mL microwave tube, moisten it with a little water and add 2mL of HNO 3 and 6mL HCl, put it at room temperature overnight, then put it in a microwave digestion furnace, program the microwave program, make it gradually rise from room temperature to 180°C and keep it for 15min, then gradually cool down to room temperature, remove it, and adjust it with NaOH solution PH to 7.4, use a 50ml volumetric flask to make up the volume, and prepare the loading solution to be tested.

[0078] 3. Sample testing

[0079] (1) Add 100mM EDTA chelating agent to the sample loading solution to be tested according to the volume ratio of 9:1, mix well, make the cadmium ion and EDTA fully chelated, and prepare the sample loading solution ...

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Abstract

The invention discloses test paper for rapidly detecting the immunochromatography of cadmium ion colloidal gold and a preparation method and application thereof. The test paper consists of a sample pad, a colloidal gold combination pad, a nitrocellulose membrane, absorbent paper and a plastic base plate; The sample pad, the colloidal gold combination pad, the nitrocellulose membrane and the absorbent paper are sequentially stuck on the plastic base plate; an anti-cadmium ion monoclonal colloidal gold label is coated on the colloidal gold combination pad; a goat anti-rat IgG antibody and a complete antigen Cd-iEDTA-BSA of heavy metal cadmium are sequentially coated on the nitrocellulose membrane; the goat anti-rat IgG antibody is used as a quality control line; and the complete antigen Cd-iEDTA-BSA of the heavy metal cadmium is used as a test line. The test paper detects residual metal cadmium in ambient soil, a water body and an aquatic product based on the immunology theory of antigen antibody; and compared with the prior detection system, the test paper has the advantages of rapidness, cheapness, convenience, sensitivity and speciality, can give a result within five minutes and is convenient to carry along to carry out field monitoring.

Description

technical field [0001] The invention relates to a detection technology for cadmium ion residues in the environment or in aquatic products, in particular to a cadmium ion colloidal gold immunochromatographic rapid detection test strip and its preparation method and application. Background technique [0002] In recent years, the development of industrial and agricultural economy has caused varying degrees of pollution to coastal aquaculture water bodies, and the heavy metal content in water bodies and aquaculture aquatic products has increased to varying degrees. Investigations have shown that lead, cadmium, copper, chromium and Residues of heavy metals such as nickel are 9, 7.5, 7.5, 4.6 and 5.1 times higher than the survey results in the early 1990s. Excessive heavy metals can mostly inhibit the activity of biological enzymes and destroy normal biochemical reactions. Heavy metals enter the body through eating aquatic products that exceed the standard. The heavy metals that e...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/551G01N33/532G01N33/558
Inventor 向军俭唐勇陈耀强王建华王宏邓宁
Owner JINAN UNIVERSITY
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