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Multi-linked immunity chromatography test paper for detecting saliva abusing drug, system and its preparation method

A technology of immunochromatographic test strips and drugs, which is applied in the field of medical testing, can solve the problems of high technical requirements, infection of sample donors, difficult sampling and sample fidelity, etc., and achieve the effect of safe operation and convenient use

Inactive Publication Date: 2008-08-13
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) Urine samples involve ethics and privacy, and there are certain difficulties in sampling and doubts about the fidelity of samples
[0006] (2) Blood samples involve ethics and trauma, and there is a certain risk of infection for sample donors
[0007] (3) The procedures for the pretreatment of tissue and hair samples are responsible. The detection methods are mostly large-scale equipment such as ELISA and GC / MS, which have higher technical requirements and are not suitable for on-site rapid detection.

Method used

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  • Multi-linked immunity chromatography test paper for detecting saliva abusing drug, system and its preparation method
  • Multi-linked immunity chromatography test paper for detecting saliva abusing drug, system and its preparation method

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preparation example Construction

[0033] Various solutions involved in the preparation of immunochromatographic test strips, such as coating buffer, blocking solution, labeling washing solution, etc., are commonly used reagents in the preparation of test strips.

[0034] Preparation of coating buffer solution: 0.05M pH9.6 sulfate buffer solution is used as the coating solution, filtered through a 0.22μ membrane, stored at 2°C to 8°C for use, and valid for one week.

[0035] Preparation of blocking solution:

[0036] Prepare 0.01M pH7.0 phosphate buffered saline (PBS), filter through a 0.22μ membrane, store at 2°C to 8°C for use, and have a validity period of one week.

[0037] Prepare blocking working solution: 2% BSA, 2% skimmed milk, 0.01M pH7.0 phosphate buffered saline (PBS), filter with 0.22μ membrane, store at 2°C-8°C for blocking coating, valid for one week.

[0038] Preparation of labeling washing solution: 2% bovine serum albumin BSA, 0.01M pH7.0 PBS solution, 0.22μ membrane filtration, set at 2°C-8°...

Embodiment 1

[0040] (1) Multi-link immunochromatography system

[0041] The multiple immunochromatographic system capable of detecting drug methamphetamine and morphine in saliva comprises a plastic casing, two detection strips for detecting drug methamphetamine and morphine in immunochromatographic saliva placed in the plastic casing, and saliva pretreatment reagents.

[0042] The immunochromatographic test strip for detection of methamphetamine and morphine in saliva consists of a sample pad, a glass fiber membrane coated with colored latex particles to mark methamphetamine and morphine monoclonal antibodies, and a coating film on the substrate. And the reaction membrane strip formed by absorbent paper, the coating film is provided with the control area (i.e. control strip) coated with anti-mouse antibody, and is coated with two detection areas (i.e. detection strip) of methamphetamine and morphine antigen respectively. ).

[0043] (2) Preparation method

[0044] The preparation method...

Embodiment 2

[0068] (1) Triple immunochromatography system:

[0069] The multiple immunochromatographic system capable of detecting cocaine, heroin, and ecstasy in saliva includes a plastic casing, a cocaine, heroin, and ecstasy joint detection reaction strip placed in the plastic casing, and a saliva pretreatment reagent.

[0070] Cocaine, heroin and ecstasy joint detection reaction test strip: it is a bottom substrate in which the sample pads are attached to each other in sequence, a glass fiber membrane coated with colloidal selenium particles to mark cocaine, heroin and ecstasy monoclonal antibodies, a coating film and The reaction membrane strip formed by absorbent paper, the coating film is provided with a control area (i.e. quality control strip) coated with anti-mouse antibody, and is coated with three detection areas (i.e. detection area) of cocaine, heroin and ecstasy antigen respectively. Bands).

[0071] (2) Preparation method:

[0072] The preparation method of the immunochr...

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Abstract

The present invention discloses a multi-linked chromatographic test paper bar for detecting drug abuse in saliva and preparation thereof. The testing paper bar is a reaction film bar made of sample mats lap jointed mutually in turn pasted on the base, glass fiber film coated with colorful latex, colloidal gold or colloid selenium particles labeled protein, custodite film and absorbent paper. Control region coated anti-rat antibody, at least two detecting region of which coated with corresponding drug antigen are set on custodite film. The present invention has merits of safety and simple operation, suitable for detecting by single person / share and high detecting speed, and multi-detection of drug antigen in saliva at the same time.

Description

technical field [0001] The invention belongs to the field of medical testing, and relates to an immunochromatography test strip, a system and a preparation method for rapid multiple detection of drugs in saliva based on the principle of immunochromatography. Background technique [0002] Drug crime is a major public hazard in today's international society. Drug abuse has become a global problem and is considered to be a plague in today's world and a cancer of society. Since the reform and opening up, with the opening of the country, international drug trafficking groups began to carry out drug infiltration activities from the border of our country. In the 1990s, it spread rapidly and became a serious social problem. And each kind of drug requires a single test, which has defects such as difficult material collection, complicated detection, and high cost. [0003] In the detection of drugs, methods such as immunochromatography, enzyme-linked immunosorbent assay (ALISA), PCR...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543G01N33/532
Inventor 王继华
Owner GUANGZHOU WONDFO BIOTECH
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