Multi-linked immunity chromatography test paper for detecting saliva abusing drug, system and its preparation method
A technology of immunochromatographic test strips and drugs, which is applied in the field of medical testing, can solve the problems of high technical requirements, infection of sample donors, difficult sampling and sample fidelity, etc., and achieve the effect of safe operation and convenient use
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[0033] Various solutions involved in the preparation of immunochromatographic test strips, such as coating buffer, blocking solution, labeling washing solution, etc., are commonly used reagents in the preparation of test strips.
[0034] Preparation of coating buffer solution: 0.05M pH9.6 sulfate buffer solution is used as the coating solution, filtered through a 0.22μ membrane, stored at 2°C to 8°C for use, and valid for one week.
[0035] Preparation of blocking solution:
[0036] Prepare 0.01M pH7.0 phosphate buffered saline (PBS), filter through a 0.22μ membrane, store at 2°C to 8°C for use, and have a validity period of one week.
[0037] Prepare blocking working solution: 2% BSA, 2% skimmed milk, 0.01M pH7.0 phosphate buffered saline (PBS), filter with 0.22μ membrane, store at 2°C-8°C for blocking coating, valid for one week.
[0038] Preparation of labeling washing solution: 2% bovine serum albumin BSA, 0.01M pH7.0 PBS solution, 0.22μ membrane filtration, set at 2°C-8°...
Embodiment 1
[0040] (1) Multi-link immunochromatography system
[0041] The multiple immunochromatographic system capable of detecting drug methamphetamine and morphine in saliva comprises a plastic casing, two detection strips for detecting drug methamphetamine and morphine in immunochromatographic saliva placed in the plastic casing, and saliva pretreatment reagents.
[0042] The immunochromatographic test strip for detection of methamphetamine and morphine in saliva consists of a sample pad, a glass fiber membrane coated with colored latex particles to mark methamphetamine and morphine monoclonal antibodies, and a coating film on the substrate. And the reaction membrane strip formed by absorbent paper, the coating film is provided with the control area (i.e. control strip) coated with anti-mouse antibody, and is coated with two detection areas (i.e. detection strip) of methamphetamine and morphine antigen respectively. ).
[0043] (2) Preparation method
[0044] The preparation method...
Embodiment 2
[0068] (1) Triple immunochromatography system:
[0069] The multiple immunochromatographic system capable of detecting cocaine, heroin, and ecstasy in saliva includes a plastic casing, a cocaine, heroin, and ecstasy joint detection reaction strip placed in the plastic casing, and a saliva pretreatment reagent.
[0070] Cocaine, heroin and ecstasy joint detection reaction test strip: it is a bottom substrate in which the sample pads are attached to each other in sequence, a glass fiber membrane coated with colloidal selenium particles to mark cocaine, heroin and ecstasy monoclonal antibodies, a coating film and The reaction membrane strip formed by absorbent paper, the coating film is provided with a control area (i.e. quality control strip) coated with anti-mouse antibody, and is coated with three detection areas (i.e. detection area) of cocaine, heroin and ecstasy antigen respectively. Bands).
[0071] (2) Preparation method:
[0072] The preparation method of the immunochr...
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