Online discovery and comprehensive antioxidation activity evaluation method for natural antioxidants in fructus chaenomelis as crude medicine

A technology of natural antioxidants and antioxidant activity, which is used in material separation, analytical materials, measurement devices, etc.

Active Publication Date: 2017-03-08
SHANDONG ANALYSIS & TEST CENT
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  • Abstract
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Problems solved by technology

On-line screening of antioxidant components based on HPLC-DPPH technology combined with antioxidant activity fingerprints for activity evaluation of traditional Chinese medicine has not been reported

Method used

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  • Online discovery and comprehensive antioxidation activity evaluation method for natural antioxidants in fructus chaenomelis as crude medicine
  • Online discovery and comprehensive antioxidation activity evaluation method for natural antioxidants in fructus chaenomelis as crude medicine
  • Online discovery and comprehensive antioxidation activity evaluation method for natural antioxidants in fructus chaenomelis as crude medicine

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Embodiment Construction

[0028] The present invention will be further described below in conjunction with accompanying drawing.

[0029] (1) Preparation of papaya extract

[0030] Take 5g of fresh papaya sample, add 10mL of methanol and bathe in water at 60°C for 10min, then add 5mL of water and mix well, then homogenize and flash extract for 1min with a homogenizer. After standing still, the supernatant was passed through a 0.22 microporous membrane to obtain papaya extract for later use.

[0031] (2) Determination of antioxidant activity of papaya extract

[0032]Dissolve DPPH in methanol, dilute to a final concentration of 0.04 mg / mL, and store at 4°C in the dark. Take 50 μL of papaya samples and 100 μL of DPPH (0.04 mg / mL) solution, mix well and react at room temperature in the dark for 30 min. Afterwards, the absorbance of the mixed solution was measured at 517nm in a microplate reader and recorded as A 样品 , the control group replaced the extract with the sample extraction solvent, and the ab...

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Abstract

The invention discloses an online discovery and comprehensive antioxidation activity evaluation method for natural antioxidants in fructus chaenomelis as a crude medicine. The method comprises the following steps: firstly, the antioxidation activity of fructus chaenomelis extracting solutions of different batches is determined; then chemical components contained in the fructus chaenomelis are measured and determined with HPLC-ESI-TOF/MS; chemical components with antioxidation activity in the fructus chaenomelis are screened out with an HPLC-DPPH online screening method, and IC50 values of part of the active components are determined; an antioxidation activity fingerprint spectrum of the fructus chaenomelis is established by means of HPLC analysis of multiple batches of fructus chaenomelis samples, and fingerprint spectrum similarity analysis is used for evaluating the antioxidation activity of different varieties of the fructus chaenomelis; multiple antioxidation active components are selected for content determination. The antioxidation activity quality of the fructus chaenomelis is evaluated preliminarily on the basis of the antioxidation capacity of the fructus chaenomelis extracting solutions, the variety difference between different varieties of the fructus chaenomelis is evaluated preliminarily on the basis of the antioxidation activity fingerprint spectrum similarity analysis, and the antioxidation activity of the fructus chaenomelis as the crude medicine is evaluated comprehensively by analyzing the content of multiple antioxidation active components.

Description

technical field [0001] The invention belongs to the field of analysis and activity evaluation of traditional Chinese medicines, and in particular relates to an online screening and identification of active ingredients of traditional Chinese medicines and an activity fingerprint combined with multi-index quantitative comprehensive antioxidant activity evaluation method. Background technique [0002] In recent years, with the improvement of people's health awareness, research on antioxidants has become a hot spot. The screening of natural antioxidants is in full swing. Many traditional Chinese medicines, such as rhodiola, licorice and mulberry leaves, contain various antioxidant components. Discovering natural antioxidants from traditional Chinese medicine has also become an important development direction. However, the traditional discovery and evaluation method of active ingredients based on natural medicinal chemistry has disadvantages such as time-consuming, high cost, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 赵恒强王晓闫慧娇赵志国于金倩刘伟耿岩玲
Owner SHANDONG ANALYSIS & TEST CENT
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