33 results about "Affinity labeling" patented technology

The invention relates to a method and test kit for quantitative determination of the amounts or relative proportions of polynucleotides in a mixture. The invention enables assessment of dynamic variations in a mixed population of organisms using affinity aided solution hybridization. The test kit comprises organized pools of polynucleotide probes having approximately the same number of nucleotides, which are distinguishable using resolution enabling tags providing the probes with different sizes. The resolution enabling tags may simultaneously act as tracer, affinity or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides. The result is hybrids, recoverable on separation aiding tools provided with counterparts of the affinity tag. After the quantitative release of the probes, the individual probes can be amplified and recorded. The method and test kit are useful for determining hygienic and epidemiologic situations and evaluating the effect of antibiotic treatment and sanitary measures.

An irreversible affinity labeling agent for labeling glutathione-S-transsulfurase sj(GST) comprises an N-(dimethylhydroquinone yloxyethyl)-(N-p-substituted amido benzoyl) glycine monovalent labeling agent, and a bisamide bivalence labeling agent which is derived by the N-(dimethylhydroquinone yloxyethyl)-(N-p-substituted amido benzoyl) glycine monovalent labeling agent and dialkyl triamine; the irreversible labeling agent includes four types of functional groups, namely a dimethylhydroquinone yloxyethyl hydrophobic orienting group for guiding the labeling agent to enter a hydrophobic site at a GST active center, a modifying functional base which is represented by alpha-halogenated acyl and reacts with sulfydryl/ amino close to the GST active center, an N-bis-substituted amino acid coupling functional base which is in amide coupling with micro-nano materials, and para aminobenzoic acid or glutamic acid or acylates of para aminobenzoic acid and glutamic acid, wherein the acylates of para aminobenzoic acid and glutamic acid can supply flexible arms required by the modifying functional group for a connecting skeleton. According to the affinity labeling agent, carboxyl/ amino of the coupling functional group is in amide coupling with the micro-nano material amino/ carboxyl, and then affinity labeling is performed on sjGST, thus achieving covalent linkage outside the active bit of fusion expression protein, and as a result, a novel protein functional micro-nano material can be prepared.