Progressive compression driven flow in vitro diagnosis method

Abstract

A cartridge and method for conducting a labeled molecular affinity binding test such as antibody / antigen, ligand / receptor, and colorometric reactions, and other chemical reactions for which one or more analytes is present in a liquid sample formate. A progressive compression structure progressively forces a liquid flow out of a conjugate pad toward a reaction region to more thoroughly and rapidlymix the liquid, encouraging specific first affinity binding to the analytes in question. A specially dimensioned constricting passageway surrounding the reaction region including the result zones provides an additional siphoning force to the flow. These combined forces rapidly and more evenly guide the flow of liquid through the reaction region so that the rushed rate of uptake of analytes at thestrip lines are more evenly distributed, adhesive attachment of non-specific molecules is largely avoided, vastly improving sensitivity and specificity, and providing quantitative results in some tests.

Description

[0001] Cross References to Related Applications [0002] This application claims U.S. Patent Application No. 15188859, filed June 21, 2016, and U.S. Patent Application No. 14887179, filed October 19, 2015 (granted, Patent No. 9377457, dated June 28, 2016) Priority and incorporated herein by reference. technical field [0003] in vitro immunodiagnosis Background technique [0004] Labeled molecular affinity binding has been developed for decades. This is a convenient and effective detection method, which is often used for screening and analysis of various substances, such as drugs, hormones, tumor markers, and various pathogens (including human immunodeficiency virus HIV) and so on. [0005] In the field of real-time detection or point-of-care test (POCT), immunochromatographic assays (Immunochromatographic assays, commonly known as colloidal gold rapid detection) using affinity molecular markers have been widely used (refer to US Patent No. 5,656,503). This is a fast, ch...

AI Technical Summary

Problems solved by technology

Lateral flow rapid diagnostic reagents, although cheap, simple and practical, lack sensitivity and accuracy

The results of lateral flow rapid diagnostic reagents will also affect the interpretation of automatic scans

For example, in the detection of saliva, the concentration of the tested substance is relatively low, and it is difficult for the lateral flow rapid diagnostic reagent to obtain accurate results.

If the traffic police conduct real-time detection on the side of the road, they cannot get accurate results in time, which will easily cause troubles in law enforcement

[0008] There are many reasons that affect the accuracy of the rapid detection of colloidal gold, mainly the uneven flow of liquid on the nitrocellulose membrane, and the retention of non-specific reactions on the membrane also interferes with the results of specific reactions

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