Preparation and application of modified bletilla striata polysaccharide derivative nano-carrier

The technology of Bletilla striata polysaccharide and derivatives is applied in the field of preparation of hydrophobically modified low-molecular-weight Bletilla striata polysaccharide derivatives and nano-carriers thereof, and achieves the effects of prolonging action time, reducing toxic and side effects, and preventing enzymatic degradation.

Inactive Publication Date: 2015-03-25
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, its modified products are difficult to be applied and popularized in new medical materials.

Method used

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  • Preparation and application of modified bletilla striata polysaccharide derivative nano-carrier
  • Preparation and application of modified bletilla striata polysaccharide derivative nano-carrier
  • Preparation and application of modified bletilla striata polysaccharide derivative nano-carrier

Examples

Experimental program
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Effect test

example 1

[0031] Degradation and modification of Bletilla striata polysaccharide:

[0032] (1) Cellulase degradation of Bletilla striata polysaccharide

[0033] Accurately weigh 2.00g of Bletilla striata polysaccharide, disperse and dissolve in 100ml of distilled water, adjust the pH to 5.0 with potassium dihydrogen phosphate, add 0.45% (w / v) cellulase, incubate at 50°C for 4-6 hours, boil for 5-10 minutes Inactivate the enzyme; the reducing sugar content of the degradation product measured by the 3,5-dinitrosalicylic acid method is 14.27±0.59%, and the viscosity-average molecular weight is 4850.48Da.

[0034] (2) Synthesis of Bletilla striata polysaccharide grafted with deoxycholic acid

[0035] Accurately weigh 0.04g of the Bletilla striata polysaccharide after the enzymatic degradation of the above cellulose, dissolve it in 20mL dimethyl sulfoxide, and set aside; weigh 0.39g of deoxycholic acid, 0.19g of N,N-dicyclohexylcarbodiimide 1. Dissolve 0.12g of 4-dimethylaminopyridine in 5...

example 2

[0037] (1) Cellulase degradation of Bletilla striata polysaccharide

[0038] Accurately weigh 2.00g of Bletilla striata polysaccharide, disperse and dissolve it in 100ml of distilled water, adjust the pH to 4.0 with potassium dihydrogen phosphate, add 0.25% (w / v) cellulase, incubate at 40°C for 4-6 hours, boil for 5-10 minutes Inactivate the enzyme; the reducing sugar content of the degradation product measured by the 3,5-dinitrosalicylic acid method is 10.60±0.38%, and the viscosity-average molecular weight is 4235.21Da.

[0039] (2) Synthesis of Bletilla striata polysaccharide grafted with lauric acid

[0040]Accurately weigh 0.08g of Bletilla striata polysaccharide after cellulose enzymatic degradation, dissolve it in 40mLN,N dimethylformamide water (5:1, v / v) mixed solvent, set aside; weigh 0.16g lauric acid, 0.19g Dissolve N,N-dicyclohexylcarbodiimide and 0.31g 4-dimethylaminopyridine in 5mL of N,N dimethylformamide water (5:1, v / v) mixed solvent, activate in ice bath 2...

example 3

[0042] (1) Cellulase degradation of Bletilla striata polysaccharide

[0043] Accurately weigh 2.00g of Bletilla striata polysaccharide, disperse and dissolve it in 100ml of distilled water, adjust the pH to 4.5 with potassium dihydrogen phosphate, add 0.35% (w / v) cellulase, incubate at 45°C for 4-6 hours, boil for 5-10 minutes Inactivate the enzyme; the reducing sugar content of the degradation product measured by the 3,5-dinitrosalicylic acid method is 12.80±0.41%, and the viscosity-average molecular weight is 3164.39Da.

[0044] (2) Synthesis of Bletilla striata polysaccharide grafted with cholic acid

[0045] Accurately weigh 0.10g of Bletilla striata polysaccharide after enzymatic degradation, dissolve it in 25mL of methanol water (4:1, v / v) mixed solvent, and set aside; weigh 0.32g of cholic acid, 0.19g of N,N-bicyclohexyl Carbodiimide and 0.14g 4-dimethylaminopyridine were dissolved in 5mL of methanol water (4:1, v / v) mixed solvent, activated in ice bath for 2h, and the...

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Abstract

The invention relates to a method for preparing a hydrophobic modified low-molecular weight bletilla striata polysaccharide derivative and application of the derivative serving as a nano drug carrier in the field of biological medicines. The method comprises the following steps: performing purification, enzymolysis and refining on crude bletilla striata polysaccharide, thereby obtaining a low-molecular weight bletilla striata polysaccharide; carrying out a catalyst assisted reaction to graft a hydrophobic group, dialyzing the reaction liquid, and performing freeze drying, thereby obtaining the hydrophobic modified low-molecular weight bletilla striata polysaccharide derivative. The obtained product can be self-assembled to form shell-hydrophilic and core-hydrophobic spherical nanoscale micelles in an aqueous solution, and the particle size of the micelles can be controlled to be in a range from 60nm to 800nm by regulating the molecular weight of the bletilla striata polysaccharide, the types of hydrophobic groups, the degree of substitution and other factors. The nano micelles can serve as a targeted delivery carrier of hydrophobic drugs and have good hepatic targeting properties.

Description

technical field [0001] The invention belongs to the field of functional medical materials and nanotechnology, and specifically relates to a method for preparing hydrophobically modified low-molecular-weight bletilla striata polysaccharide derivatives and nano-carriers thereof. technical background [0002] Bletilla striata polysaccharide is extracted from the traditional Chinese medicine Bletilla striata, which is the main active ingredient in astringent, hemostasis, detumescence and muscle growth. Studies have shown that Bletilla striata polysaccharide has biological activities such as hemostasis, promotion of wound healing, antibacterial, anti-inflammatory, anti-tumor, anti-oxidation, anti-ulcer, and promotion of bone marrow hematopoiesis, and has a wide range of clinical applications. In addition, it can also be used as a functional additive for food and cosmetics. In recent years, scholars at home and abroad have devoted themselves to developing Bletilla striata polysac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/10A61K47/36A61P1/16
Inventor 王文苹贺少龙洪彤彤刘艳华隋宏
Owner NINGXIA MEDICAL UNIV
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