Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Quantitative detection method of polysaccharide containing uronic acid

A technology for quantitative detection of uronic acid polysaccharides, which is used in measurement devices, instruments, scientific instruments, etc., can solve the problem that the degrading enzymes of glycosaminoglycanase are expensive, the polysaccharide content cannot be determined, and the chemical color development method lacks specificity, etc. problem, to achieve the effect of simple operation, low cost and accurate results

Active Publication Date: 2016-06-15
DALIAN POLYTECHNIC UNIVERSITY
View PDF4 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these chemical chromogenic methods lack specificity. For samples containing several polysaccharides of this type at the same time, only their total amount can be measured, and the content of each polysaccharide cannot be determined.
It has been reported in the literature that the disaccharides produced by the enzymatic hydrolysis of glycosaminoglycans were quantified by high performance liquid chromatography-liquid mass spectrometry, thereby quantifying various glycosaminoglycans, but the price of the degrading enzyme of glycosaminoglycanase are very expensive, and there is no commercial degrading enzyme for polysaccharides containing uronic acid-hexose disaccharide fragments, so this method is difficult to be widely used

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quantitative detection method of polysaccharide containing uronic acid
  • Quantitative detection method of polysaccharide containing uronic acid
  • Quantitative detection method of polysaccharide containing uronic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Step 1: Accurately take about 10 mg each of heparin, chondroitin sulfate, hyaluronic acid, and abalone gonadal polysaccharide AGSP. After mixing, add 5 ml of water, dissolve, overnight, and dilute to 10 ml volumetric flask with 2.6 mol / L trifluoroacetic acid. Shake well to make standard mother liquor. Then dilute it with 1.3mol / L trifluoroacetic acid, and prepare mixed standard polysaccharide solutions with serial concentrations (0.02, 0.04, 0.06, 0.08, 0.10, 0.20, 0.40, 0.6, 0.8, 1.0mg / ml), each concentration is two parallel.

[0037] Step 2, prepare the polysaccharide solution of the sample to be tested: take 100 mg of the contents of glucosamine chondroitin sulfate calcium capsules in a 25ml volumetric flask, dilute to volume with water, shake well, pass through a 0.22 μm filter membrane overnight, and obtain the polysaccharide solution of the sample to be tested; accurately measure Take 0.50ml of the polysaccharide solution of the sample to be tested in a 5ml hydro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a quantitative detection method of polysaccharide containing uronic acid. The method comprises the following steps: preparing a standard polysaccharide solution and a sample solution to be tested; hydrolyzing polysaccharide in the standard solution and the sample solution to be tested so as to obtain hydrolyzed residue; derivatizing the residue with 1-phenyl-3-methyl-5-pyrazolone to prepare a sugar derivative; carrying out chromatographic analysis on the sugar derivative by the utilization of a high performance liquid chromatograph and a triple quadrupole linear ion trap mass spectrometer; determining which kind of polysaccharide containing uronic acid the sample to be tested is according to the chromatogram; and preparing a standard curve of chromatographic peak area and polysaccharide concentration according to the chromatogram of the standard polysaccharide disaccharide derivative obtained by high performance liquid chromatography-mass spectrum, and calculating content of polysaccharide containing uronic acid in the sample to be tested by the utilization of peak area of the sample to be tested. According to the method provided by the invention, quantitative detection of polysaccharides can be accurately carried out, and effective means is provided for quality control of products such as medicines, health product and the like of the polysaccharides.

Description

technical field [0001] The invention belongs to the technical field of uronic acid polysaccharide detection, in particular to a method for quantitative detection of uronic acid polysaccharides. Background technique [0002] The repeating disaccharide in the structure is a polysaccharide composed of an uronic acid and a hexosamine or hexose, which widely exists in nature. For example, heparin, chondroitin sulfate, and hyaluronic acid in glycosaminoglycans are all composed of repeating disaccharide fragments linked by uronic acid and hexosamine. In addition, polysaccharides containing repeated disaccharide fragments formed by linking uronic acid and hexose have also been found in some shellfish and other organisms, such as abalone gonad polysaccharide AGSP, etc. Due to their significant functional activity, some uronic acid-containing polysaccharides have been developed as medicines and health products. For example, chondroitin sulfate can effectively prevent arthritis, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8836
Inventor 宋爽张豹刘海曼鲁娇娇刘斌屈福杰周大勇
Owner DALIAN POLYTECHNIC UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com